Presentation on theme: "Anabolism: What micro- organisms do with all that 1. Reducing power 2.Energy 3. Raw building materials."— Presentation transcript:
Anabolism: What micro- organisms do with all that 1. Reducing power 2.Energy 3. Raw building materials
Reducing Power NADH/FADH glycolysis and Kreb’s cycle NADPH oxidative photophosphorylation NADPH anaerobic photophosphorylation 1. Reverse electron flow 2. Protons from inorganic molecules (H 2 S, S 2 O 3 ) Protons leached from Fe-S containing molecules in PSI in anaerobic photo-phosphorylation
Energy--ATP 1. Oxidation of organic molecules (glycolysis/Kreb’s cycle) 2. Electron transport: A. oxidative phosphorylation (PMF) chemiosmosis)— oxygen the final proton/electron acceptor B. anaerobic– inorganic molecules final electron acceptor 3.Oxidation of inorganic molecules (usually fed into electron transport chain) 4. Dissimulative reduction of inorganic molecules (CO 2, sulfides and iron 5. Oxidative and anaerobic photophosphorylation
Raw building materials Carbon/Nitrogen/Phosphorous etc. from the catabolism of carbohydrates lipids proteins nucleic acids strange organic matter such as petroleum Carbon from CO2 fixation Nitrogen from Nitrogen (N2) fixation Etc.
One strange thing bacteria can eat for energy and biomass—Petrol !!! Mediated by oxygen and the enzyme mono-oxygenase variety of yeasts/ molds and bacteria
Another strange thing—benzene/toluene Requires oxygen, NADH. Aliphatic carbons are converted to linear molecules that can be catabolized to succinate/Acetyl-CoA/pyruvate which enter Kreb’s cycle—Pseudomonas spp.
CO 2 fixation (Calvin cycle, reverse TCA cycle and hydroxy-proprionate cycle) How autotrophs reduce CO 2 into material they can use for biomass.
Calvin cycle In: CO2, NADPH and ATP Out: Fructose-6 phos intermediate of glycolysis
Reverse TCA cycle
Nitrogen fixation: atmospheric nitrogen to fixed nitrogen that can be utilized by plants and bacteria
Agricultural significance of nitrogen fixation: Crop rotations with plants that form intimate symbiotic relationships with Rhizobium spp. (bacteria in root nodules) peas, beans, soybean, alfalfa, clover Pre seed watery environments with cyanobacteria (Anabaena azollae) and the water fern Azolla. Plant rice as rice plants grow they crowd out and kill the water fern thus releasing the nitrogen that they require pgs in text book