Presentation on theme: "Liver Function Tests (LFTs)"— Presentation transcript:
1 Liver Function Tests (LFTs) Prepared byHamad ALAssaf
2 Routine Liver Function Tests (LFTs) LFTs are blood tests used to diagnose & monitor disease or damage of the liver:1- Serum Albumin2- Blood Liver Enzymes:- Alanine amino transferase (ALT)- Aspartate amino transferase (AST)- Gamma glutamyl transferase (GGT)- Alkaline phosphatase (ALP)3- Blood Billirubin (total, direct & indirect)4- Blood Coagulation Factors (prothrombin): Prothrombin Time (PT)5- Markers of liver fibrosis
3 Serum AlbuminAlbumin is present in higher concentrations than other plasma proteins ( ~ 40 g/L in normal adults).Albumin is synthesized in the liver & has a half-life of 20 days.Very small amounts of albumin cross the glomerular capillary wall.Accordingly, no more than traces of albumin may normally appear in urine that can not be detected by ordinary laboratory means.Albuminuria : In this case, albumin can be detected in urine by ordinary laboratory means due to physiological or pathological conditions.
4 Serum Albumin Causes of hypoalbuminemia: Artifactual: Diluted sample Physiological : PregnancyDecreased amino acids: Reduced essential amino acids in diet & reduced synthesis of nonessential amino acids due to either Malnutrition or Malabsorption.Increased catabolism : Surgery, Trauma, Infections.Defective synthesis in liver: Chronic liver diseases (liver cirrhosis)Increased loss : From the kidney (Nephrotic syndrome) or From GIT (Protein loosing entropathies)
5 Blood Aminotransferases (ALT & AST)Aminotransferases (ALT & AST) are normally intracellular enzymes.Elevated blood levels of aminotransferases indicate damage to cells rich in these enzymes (as disease to tissue or physical trauma )Blood AST & ALT are of particular diagnostic value
6 Causes of elevated levels of blood ALT & AST 1- Viral , toxic or alcholic hepatitis:Highly increase in ALT & AST (Up to folds).In viral hepatitis, ALT is much elevated than AST2- Cirrhosis (chronic liver diseases):Moderate increase (up to 4 – 5 folds)In chronic cases, AST is much elevated than ALT.3- Obstructive jaundice:Moderate increase ALT & AST are increased up to 3 folds.4- After alcoholic or drug intake:Transient slight to moderate increase.
7 Alanine amino transferase (ALT) Aspartate transaminase (AST) ALT is more liver specific than AST.ALT rarely increases in lesions other than the liver parenchymalALT elevations persist longer than do AST.Formerly named as Glutamate pyruvate transferase (GPT)Blood levels of AST are increased with many diseases of various organs:1- Liver diseases2- Myocardial infarction (MI)3- Progressive skeletal muscular dystrophy4- Crush injury5- Hemolytic diseases6- Artifact: in hemolysed samples or if serum separation is delayed.Formerly named as Glutamate oxaloacetate transferase (GOT)Aspartate transaminase (AST)
8 Gamma glutamyl transferase (GGT) GGT present in blood originates primarily from hepatobiliary systemCauses of increased blood GGT:1- Induction of GGT synthesis by these cells occurs without cell damageby alcohol or drugs as anticonvulsants.2- Biliary obstruction:GGT is markedly increased with obstructive jaundice (5 – 30 folds)Increase earlier (more sensitive) than ALPPersists longer than ALP3- Viral, toxic & alcoholic hepatitis :Increase is only 2 – 5 folds (less sensitive than ALT & AST)4- Primary and secondary liver tumors:GGT is elevated earlier than other enzymes in liver neoplasm.Secondary of other organ tumors in the liver can be early detected by elevated GGT. (arouse suspicious that the diseases is metastatic to liver)
9 Alkaline Phosphatase (ALP) Main Sources of ALP:1- Cells of hepatobiliary tract (hepatocytes adjacent to the biliary canalculi).2- Osteoblasts of bone :3- Other Sources: Intestine and placenta & renal tubules.If ALP is elevated due to a bone diseaseIn this case, GGT is normali.e. GGT is used to ascertain whether increased ALP is due to bone or hepatobiliary disease
10 Alkaline Phosphatase (ALP) cont. Clinical significance of increased serum ALP activities:1- Physiological increase of ALP:During periods of active bone growth in infancy and at puberty.Preterm infants total ALP is increased to 5 times the upper reference limit of adults due to bone isoenzymes.In children under 3 years, total ALP activity is increased up to 2.5 times the upper limit.Increased twice, during the second and third trimesters of pregnancy (placental ALP).
11 Alkaline Phosphatase (ALP) cont. 2- Pathological increase of ALP:A- Bone causes: (due to increased osteoblastic activity):Tumors (osteogenic)Paget`s Disease of bone: Marked increase (10 – 25 folds)Primary osteogenic tumorsSecondary malignant deposits in bone if causing osteoblastosisRickets & osteomalacia (vitamin D deficiency)Primary & secondary hyperparathyroidism (increased PTH)Healing of bone fractures
12 Alkaline Phosphatase (ALP) cont. B- Hepatobiliray tract: liver diseases with involvement of biliary tract.1- Obstructive jaundice:Extrahepatic cholestiasis : (Marked increase, up to 10 – 12 folds)Due to obstruction of to the flow of bile through the biliary tract e.g. Gallstones, cholecystitis.Intrahepatic cholestiasis: (Moderate increase , ~ 3 -5 folds)Bile secretion from the hepatocytes into the canalculi is impaired e.g. cholangitis.2- Viral, toxic & alcoholic hepatitis:Mild to moderate increase, less than 3 folds.
13 Coagulation factorsThe liver makes many of the proteins (clotting factors) needed to make blood clot.In certain liver disorders the liver cannot make enough of these proteins and so blood does not clot so well.Therefore, blood clotting tests may be used as a marker of the severity of certain liver disordersIn liver disease, the synthesis of prothrombin & other clotting factors is diminished prolonged prothrombin Time (PT)This may be one of the earliest abnormalities seen in hepatocellular damage, since prothrombin has a short half-life (~ 6 hours)
14 Markers of Liver Fibrosis Procollagen type III terminal peptideHyaluronic acid (hyaluronin)
15 -Fetoprotein One of the major plasma proteins in fetal life. Falls thru-out gestation and by age one yearIn acute hepatic injury AFP 10 – 20 folds.Used to screen and diagnose Hepatocellular carcinoma & hepatoblastoma.
16 AmmoniaAmmonia is produced by all tissues from the catabolism of amino acidsAmmonia is mainly disposed is via formation of urea in liverBlood level of ammoina must be kept very low, otherwise, hyperammonemia & CNS toxicity will occur
17 catabolism of amino acids Small amount excreted in urine With production ofAmmoniaIn LiverSmall amount excreted in urineUrea
18 Hyperammonemia Increase of ammonia level of blood Normal level of blood ammonia is 5-50 mmol/LHyperammonemia : A medical emergency as ammonia has a direct neurotoxic effect on CNSAmmonia intoxication: It is defined as toxicity of the brain due to increase in ammonia level in the systemic blood.At high concentrations, ammonia can cause coma & death
19 Causes of Hyperammonemia 1- Liver diseases: are common causes in adultsi- Acute causes: viral hepatitis, ischemia, hepatotoxinsii- Chronic causes: liver cirrhosis due to alcoholism, hepatitis, biliary obstruction.2 - Gatrointestinal Bleeding:By action of bacteria of GIT on blood urea with production of much amounts of ammonia that is absorbed to blood.3- Ornithine transcarbamoylase deficiency (Hereditary)
20 Hyperammonemia in Renal Failure Renal Failure blood urea levels are elevated Transfer of urea to intestine is increased Much amounts of Ammonia is formed by bacterial urease Absorbed to blood Hyperammonemia
21 Precautions resampling, handling A free‐flowing venous (or arterial) blood sample should be collected into a specimen tube (preferably pre‐chilled) containing either lithium heparin or EDTAAs difficult venepuncture can cause a spurious increase in [ammonia].The sample should be transported on ice to the laboratory, separated within 15 minutes of collection and analyzed immediately.These precautions are necessary as the [ammonia] of standing blood increases spontaneously, due to generation and release of ammonia from red blood cells
23 Formation of Bilirubin from Heme Breakdown of RBCsHemeBiliverdin (green)Bilirubin (red-orange) bile pigmentsIn Blood with albuminUNCONJUGATED BILIRUBIN (or INDIRECT BILITUBIN)
24 Bilirubin Metabolism in the Liver Uptake of Bilirubin by hepatocytes:Bilirubin dissociates from its carrier albumin & enters hepatocytesConjugation of Bilirubin:In hepatocytes, bilirubin is conjugated with two molecules ofglucuronic acid by the enzyme glucuronyl transferaseExcretion of bilirubin into bile:Conjugated bilirubin (Direct bilirubin) is transported into bile canalculi& then into bile.
25 Bilirubin Metabolism in the Intestine Conjugated bilirubinbacteriain the intestineUrobilinogenStercobilin Reabsorbedin stool(brown)KidneyUrine Urobilin (yellow)
26 JaundiceYellow color of skin, nail beds & sclera caused by deposition of bilirubin secondary to increased bilirubin levels in blood (hyperbilirubinemia)
27 Types of Jaundice1- Hemolytic Jaundice: Massive lysis of RBCs in hemolytic anemia e.g. sickle cell anemia & G6PD deficiency anemia & Hemolytic transfusion reaction. 2- Obstructive Jaundice: Conjugated bilirubin is prevented from passing to the intestine. As in Gallstones. 3- Hepatocellular Jaundice: Liver damage (by hepatitis or cirrhosis) causes low conjugation efficiency leading to increased unconjugated (indirect) bilirubin in blood.
28 LABORATORY INVESTIGATIONS IN TYPES OF JAUNDICE UrineBloodUrobilinogenBilirubinIndirect bilirubinDirect bilirubinAST & ALTALP & GGTHemolyticjaundiceIncreasedNilincreasedNormalObstructive jaundiceDecreased or absentPresentNormal or mild increasedMarked increasedHepatocellular jaundiceNormal RangeIndirect Bilirubin< µmol/LDirect Bilirubin< 13.7 µmol/L
29 Jaundice in Newborns In newborns (especially premature) Bilirubin accumulates as the liver enzyme bilirubin glucuronyl transferase(responsible for conjugation of bilirubin) is low at birth. (The enzymesreaches adult levels in about 4 weeks).Accordingly, unconjugated bilirubin is increased in blood.Elevated bilirubin in excess of the binding capacity of albumin can diffuse into basal ganglia & cause toxic encephalopathy (kernicterus)TreatmentExposure of the newborn skin to blue fluorescent light which converts bilirubin to more polar & hence water-soluble isomersThese isomers can be excreted into bile without conjugation to glucuronic acid.
30 Congenital hyperbilirubinemia Bilirubin is elevated in blood due to inherited defects in the bilirubin metabolic pathway1- Crigler-Najjar syndromeLow activity of glucoronyltransferase (conjugating enzyme)Severe hyperbilirubinemia in neonates (unconjugated bilirubin), Complicated by kernicterus & early death2- Gilbert`s syndromeDecreased production of glucoronyltransferaseMore common in men, Occurs in 2-3 % of men.Usually asymptomatic hyperbilirubinemia with Normal Liver function tests.3- Dubin-Johnson syndromeDefect in transfer of conjugated bilirubin into the biliary canalculi Conjugated hyperbilirubinemia.