6 Advantages of using ammonia-oxidizing bacteria (AOB) Oxidize methane to methanol via the nonspecific action of the enzyme ammonia monooxygenaseContaminants such as moisture and CO2 do not post a limitation for biological conversionCan utilize the CO2 contained in gas mixtures for cell synthesis
8 Methanol Production by AOB Methanol production rate varies for conditions and performanceMaximum specific productivity is 0.82 mg methanol/ mg biomass (COD)/d
9 Industrial Challenges Microorganisms are limited.Inhibition on cell growth by H2S when methane in biogas is used.High-cost electron donors required for conversionsGas-Liquid Mass transfer limitationsNH3 may inhibit the growth of microorganisms including methanotrophs
10 Potential Strategies Microbial electrosynthesis Methanotrophic strains from AD systemsGenetic engineering of ANMEPotential StrategiesChanges in the reactor design by using Trickling biofilters for enhanced methane supply
15 ReferencesFei, Q. G., Michael. Tao, Ling. Laurens, Lieve. Dowe, Nancy. Pienk, Philip T.os. (2014). Bioconversion of natural gas to liquid fuel: Opportunities and challenges. 32(3), 596–614. doi: /j.biotechadvGe, X., Yang, L., Sheets, J. P., Yu, Z., & Li, Y. (2014). Biological conversion of methane to liquid fuels: Status and opportunities. Biotechnology Advances, 32, doi: -Pen, N. S., L. Belleville, M.-P. Sanchez, J. Charmette, C. Paolucci-Jeanjean, D. (2014). An innovative membrane bioreactor for methane biohydroxylation. Bioresource Technology, 174, 42–52. doi: /j.biortechThorn, G. J. S. (2005). Development of an Immobilized Nitrosomonas europaea Bioreactor for the Production of Methanol from Methane - thesis_fulltext.pdf. Department of Chemical Process Engineering. University of Canterbury. Retrieved from
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