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Microbial Methanol Synthesis Team 3: Robert Clifford, Patricia Firmin, Gen Liang, Rooma Raza.

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Presentation on theme: "Microbial Methanol Synthesis Team 3: Robert Clifford, Patricia Firmin, Gen Liang, Rooma Raza."— Presentation transcript:

1 Microbial Methanol Synthesis Team 3: Robert Clifford, Patricia Firmin, Gen Liang, Rooma Raza

2 Methanotrophs

3 Where Do These Bacteria Live? Wetlands Sewage Lake BasinBogs

4 Methanotroph Groupings Group I: Ribulose Monophosphate CycleGroup II: Serine Cycle Inhibit Enzyme MDH Optimize Enzyme MMO

5 Methane Monooxygenase pMMO – Particulate Methane Monoxygenase sMMO – Soluble Methane Monoxygenase

6 Advantages of using ammonia-oxidizing bacteria (AOB) Oxidize methane to methanol via the nonspecific action of the enzyme ammonia monooxygenase Contaminants such as moisture and CO2 do not post a limitation for biological conversion Can utilize the CO2 contained in gas mixtures for cell synthesis

7 Metabolism of AOB

8 Methanol Production by AOB Methanol production rate varies for conditions and performance Maximum specific productivity is 0.82 mg methanol/ mg biomass (COD)/d

9 Industrial Challenges Microorganisms are limited. Inhibition on cell growth by H2S when methane in biogas is used. High-cost electron donors required for conversions Gas-Liquid Mass transfer limitations NH3 may inhibit the growth of microorganisms including methanotrophs

10 Changes in the reactor design by using Trickling biofilters for enhanced methane supply Methanotrophic strains from AD systems Genetic engineering of ANME Microbial electrosynthesis Potential Strategies

11 Outlook for Microbial Conversion

12 Batch and Semi-batch Production

13 Membrane Semi-batch Process

14 Methanol Production Results

15 References Fei, Q. G., Michael. Tao, Ling. Laurens, Lieve. Dowe, Nancy. Pienk, Philip T.os. (2014). Bioconversion of natural gas to liquid fuel: Opportunities and challenges. 32(3), 596–614. doi: /j.biotechadv Ge, X., Yang, L., Sheets, J. P., Yu, Z., & Li, Y. (2014). Biological conversion of methane to liquid fuels: Status and opportunities. Biotechnology Advances, 32, doi: - Pen, N. S., L. Belleville, M.-P. Sanchez, J. Charmette, C. Paolucci-Jeanjean, D. (2014). An innovative membrane bioreactor for methane biohydroxylation. Bioresource Technology, 174, 42–52. doi: /j.biortech Thorn, G. J. S. (2005). Development of an Immobilized Nitrosomonas europaea Bioreactor for the Production of Methanol from Methane - thesis_fulltext.pdf. Department of Chemical Process Engineering. University of Canterbury. Retrieved from


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