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Automated Mini-Store System for Low-Temperature Samples Storage.

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Presentation on theme: "Automated Mini-Store System for Low-Temperature Samples Storage."— Presentation transcript:

1 Automated Mini-Store System for Low-Temperature Samples Storage

2 Lab Automation solutions by PAA/Biosero Focused offerings for high-throughput screening applications

3 Automated Samples Storage Systems Range of capacities and functionalities Ultra-low temperature robotic sample storage Automation integration – Front/back-end

4 Controlled-Environment Chambers Targeted for manual and automated work-processes Dry, inert, oxygen-free, ionized, temperature-controlled atmospheres possible Dry, nitrogen atmosphere for automated liquid-handling workcell Dry, nitrogen atmosphere for samples weighing

5 Automated Mini-Store (AMS) For small-medium libraries

6 Standalone, self-contained, mobile Configurable for range of tubes and vials Cherry-picking capability Medium-capacity; expandable Modular design General features

7 AMS Features and Specifications 1/2 System Size: 8’ wide, 5’ deep, 6.5’ tall Capacity: 300,000 micro-tubes (0.7ml) Throughput: 4000 picks/day; higher possible Cherry-picking: Yes, for 96- and 384-well Operating environment: o +4ºC to -20 º C (settable) o -80 º C option available Environment Backup: Liquid Nitrogen

8 AMS Features and Specifications 2/2 Input/Output Station: Positions for 6 racks Output Stacker: 24 racks capacity Compression/Consolidation: o On-demand or auto Database: Any SQL database Samples ID: 2D matrix and 1D barcode

9 Container Types and Capacity 96-well Tubes, Half-height (0.7 ml) 175,000 tubes (max 190,000) 96-well Tubes, Standard-height (1.4 ml) 275,000 tubes (max 300,000) Picoplates (or Microtiter plates) 1.9 Million picotubes 2.6 Million in stacked configuration Other Container Types Vials, custom racks and tubes are possible

10 Multi-Container Types in Single AMS AMS can easily be configured to simultaneously handle multiple container types in a single system. This is achieved by using gripper-changers at the Picking Robot for handling each container-type (similar to our larger ASRS) Note, however, that this affects the overall throughput of the system (and some loss of flexibility)

11 Modules View – Main Discrete Modules Storage Module #1 Storage Module #2 Picking Module Input/Output Module

12 Modules View – Storage Stack Storage Stack Single Pan

13 Modules View – Picking Module Extractor Extractor transfers product from storage module to picking module Picker Picker (robot) cherry-picks samples from source-racks to destination racks

14 Modules View – Pan in Extractor

15 Picking Module – Picking Station I/O Buffer I/O Buffer can hold 6 racks Cherry-Picker Can pick tubes and vials of various sizes

16 Pan Extracted for Picking Extractor Picker Source Rack I/O Module

17 Modules View – I/O Module System display I/O Stacker Rack Scanner

18 Dry Inert enclosure for plate thawing Inert / dry plate exit hotel Dry Nitrogen atmosphere Room Temperature thawing Recirculation with inline warming & desiccating module. Intelligent control with O2 and humidity sensors to monitor environment Minimal N2 consumption

19 Sample Retrieval from the Inert hotel The available capacity of the Hotel allows for batch loading of tube racks into the AMS. Tube racks can be efficiently handled in batches using ‘Cassettes’, with a capacity of 10 tube racks each. The antechamber allows product transaction without compromising the internal atmosphere

20 Cycle Times The adjacent graph shows the dependency of cycle-time to picking efficiency. If the inventory is fragmented, or if pick request is characterized by dispersed samples, the throughput will be lower. For pan extractions in the range of 1 to 20, the steep slope of the graph underlines the advantages of condensed/optimized storage. Important note: these numbers are for serial service of tube racks (not picked in batches, which AMS can do, up to 6 tube racks at once). With batch picking, throughput will be higher.

21 AMS System Features FeatureRANDEX AMS ThroughputCherry-pick of 4 96-well tube racks per hour, on average (see conditions) TemperatureYes, settable between +4C and –20C Redundancy/backupIn case of power failure or refrigeration system failure, liquid nitrogen backup (option) Humidity ControlBuilt-in dehumidification control Container Types, Capacities 300,000 0.65ml Micronic micro-tubes in their racks: (height: 32 mm); 267,264 0.75ml Matrix micro-tubes in their racks: (height: 40 mm) Cherry-PickingMicro-tubes are cherry-pickable. Cherry-picking is done inside the controlled environment System Size and weightAMS Standard Size: 8’ wide, 5’ deep, and 6.5’ tall. Weight: 2,500 lbs AMS with Integrated Hotel: Barcode scanning1D barcode scanning of racks. 2D cluster scanner for identification of microtubes Input/OutputAMS Standard via front stacker (24 tube racks), or serially. AMS with Integrated Hotel: automatic transfer of tube racks to and from Liconic LPX-200 Hotel. Hotel capacity is 100 tube racks Compression/ Consolidation Micro-tube compression. Can also compress by custom-defined field (project/department) Inventory DataWill reside in your enterprise database or in our MSDE (SQL) database Service Support24 hour turnaround in support and service

22 Process – Storing Plates/Racks Process for Storing Racks with 2D ID underside

23 Process – Retrieving Samples

24 Software/Data Integration to LIMS We can integrate our systems seamlessly into your LIMS

25 Data Model

26 Inert Sample Processing Human and Robotic Solutions

27 A Precipitous Problem WebData from water absorption vs. time (hrs) WebData from water absorption vs. time (hrs) Samples absorb 10% water in 4 hours under ambient conditions This will prevent freezing @ 20 C and compound precipitation Sample precipitation occurs under such sub-optimal conditions HTS and hit-to-lead follow-up are compromised Consensus is that dry sample processing is a component of valid compound management for HTS WebData from Waybright et al., NCI sample precipitation

28 Precipitation = More False Negatives How Much Does a False Negative Cost? Opportunity and Operations Loss

29 Solutions to a Common Problem Cost:Benefit Good: Dry boxes for thawing plates Better: Semi-automated processing in inert enclosure State of the Art: ASRS and Integrated Workcell

30 Summary: Dry Box Solution

31 Obligatory for thawing/conditioning plates Rapid dry equilibration of chamber Gentle, ‘Power Thaw’ option <1% Relative Humidity achievable Requires only power outlet Movable, space efficient and affordable Vastly superior to passive desiccant Custom configurations and options available

32  1/10 cost of alternative solutions by redeploying existing equipment Input Airlock Mobile (casters) Height adjustable Pipettor Sealer Output airlock Stackers Glove ports Inert/dry atmosphere controls Semi-Automated (‘Hubotic’) Solutions

33 Summary:‘Hubotic’ Solution Powerful workflow model. Simple and very cost-effective Pressurized with dry argon gas, data monitored and logged Inert processing from solubilization to seal Movable, ergonomically adjustable Continuous Batch Processing without breaking the conditioned environment

34  Turnkey System to your specifications  Compact, movable, cost-effective  From an established leader in Materials Management Fully Integrated Robotic Solution

35 Automated Mini-Store (AMS) Plates Processing Workstation (PPW) Buffer Stack for Random Access (of 384-well Plates) Transfer Robot Buffer Stack for Empty Plates Buffer Stack for Thawing Racks Shuttle between AMS and PPW Liquid Handling System Air-Lock Operational Schematic PlateLoc Plate Sealer

36 Compound Management Solutions Samples must be processed in dry environment o Simple solution is the DB500 for manual processing o prevents water absorption during plate thawing Continuous Batch Processing with Enclosure o emulates robotic solution (‘Hubotic’) at low cost Fully Integrated AMS: Best of both worlds o Turnkey ASRS integrated turnkey sample processing o Attractively priced, compact footprint Service and support Tried and tested technology

37 Appendix - (384-well) Picoplate Field-proven, patented design Source plate is single molded part – Low cost Cherry pick capability – no freeze-thaw cycle Source Plate Bite and Place Destination plate

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