1. Determining Ruminant Materials In Feed Ingredients Larry L. Whitlock, Sr. Ph. D. Office of The Texas State Chemist College Station, Texas 77841 ASFFPCO Annual Meeting June 22-25, 2003 Fort Worth, Texas

4. Microscopy Equipment Compound Microscope Steroscopic Microscope Reagents for Spot Tests Accessories

7. Microscopic Procedures Use a 20 gram Sample for Viewing with the Stereomicroscope Take a Separate 10 gram Sample for Floatation Observe Mineral Portion Spot Tests for Confirmation

10. Spot Testing Reagants

11. Examples of Spot Testing 10% HC1 for Carbonates, Limestone, or Oyster Shell Nesslers Reagent for Ammonium Containing Compounds Urea Spot Testing Silver Nitrate for Chlorides Quimociac for Phosphates NOTE:Work with all floatation procedures in a hood to prevent breathing the vapors from the solvent

12. Application of Microscopy Levels of Meat and Bone in Feed Sample Identify Milk and Dried Blood Microscopy is a Rapid Procedure OTSC Current Surveys

13. Meat and Bone in Feed

14. Meat and Bone Meal in Feed

17. Bone Fragment

18. Animal Bone in Feed

19. Blood Meal in Feed

20. Meat and Bone Meal

21. Poultry Meal

22. Feather Meal

23. Hydrolyzed Feathers

24. Agri-Screen by Neogen Corporation Neogen has Two Test Strips Presence of Ruminant Meat and Bone in Animal Protein Products Presence of Ruminant Meat and Bone in Feeds

25. Agri-Screen Procedures for Ruminant M&B Meal Weigh 10 grams Sample in Mason Type Jar Add 100 ml Extraction Solution Place in Boiling Water Bath for 10 Minutes Remove Mason Jar from Water Bath Prepare Sample Tubes and Transfer 0.5 ml of Extract to a Tube (Use different pipette for each sample) Place Sample Strip into each Sample Tube Develop for 10 Minutes and Read Results

27. Reading Strip Results Negative-Control Line Appears Only Postive-Control Line and Sample Line Appear

32. Discussion