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CELL DIVISION IN MICROGRAVITY By Natasha Garamani, Jennifer Jiang, Jasmine Kuo, Kara Lukas, Elyssia Widjaja.

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Presentation on theme: "CELL DIVISION IN MICROGRAVITY By Natasha Garamani, Jennifer Jiang, Jasmine Kuo, Kara Lukas, Elyssia Widjaja."— Presentation transcript:

1 CELL DIVISION IN MICROGRAVITY By Natasha Garamani, Jennifer Jiang, Jasmine Kuo, Kara Lukas, Elyssia Widjaja

2 Background  Cell division is an integral component of life.  All organisms must go through the cell cycle in order to grow, develop, and reproduce.  Past experiments that have been conducted on Earth in simulated microgravity have shown that the lack of gravity causes cells to divide at a slower rate.

3 Purpose  To discover the effect of microgravity on cell division  To compare the rates of cell division in the absence and presence of gravity

4  If cells are exposed to microgravity during their life cycles, then there will be a reduction in cell division because of  disorganization of microtubules  change in actin filaments  chromatin less dense  increased apoptosis Hypothesis

5 Materials  Fibroblasts  Eagle’s Minimum Essential Medium (EMEM)  1% Fetal Bovine Serum (FBS)  Light Microscope  Hemocytometer  Pipette  Trypan Blue

6 Procedures - Ground Experiment 1. Preparation of flasks  Coat inside of flasks with 3 mL of poly-lysine solution for 5 minutes  Aspirate the solution and let it dry overnight  Transfer 500 fibroblast cells into the flask with a pipette  Add 5 mL of EMEM with 1% FBS  Incubate cells overnight

7 Procedures - Ground Experiment 2. Initiation of Experiment  Add 6.3 EMEM mixed with 1% FBS to both flasks  Keep one flask in the incubator and put the other flask into a controlled room temperature environment  Allow the cells to continue to grow and divide for 12 days

8 Procedures - Ground Experiment 3. Counting the Cells  Wash the cells with 2 ml of Phosphate-Buffered Saline solution  Add 0.5 ml trypsin/EDTA to cause disassociation of the cells from the flask and rotate for 1 minute.  Allow the cells to detach and add 1 ml of PBS.

9 Procedures 3. Counting the Cells (continued)  Remove medium from the flask, place it in a 15 ml centrifuge tube, and spin for 5 minutes in a tabletop centrifuge to pellet the cells.  Resuspend the pellet in 0.5 ml of PBS  Stain culture sample with trypan blue  Count the cells collected from culture using a hemocytometer.

10 Data  Initial Count  500 cells (per flask)  Final Count  Avg cells  Initial Count  500 cells (per flask)  Final Count  Avg cells  Initial Count  750 cells (per flask)  Final Count  Avg cells Room TemperatureIncubator

11 Process of the Experiment

12 Conclusion  Growth is more prominent for cells in incubator (at 37 degrees Celsius)

13 Acknowledgements  Mr. Wyeth Collo for his guidance as our advisor.  Dr. Susan Kane and Ms. Erin Denny of City of Hope Cancer Center, Developmental Cancer Therapeutics Program, Comprehensive Cancer Center for providing the lab space and materials.  Smithsonian Air and Space Museum for hosting this event.  SSEP for this invaluable experience.


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