Presentation on theme: "CELL DIVISION IN MICROGRAVITY By Natasha Garamani, Jennifer Jiang, Jasmine Kuo, Kara Lukas, Elyssia Widjaja."— Presentation transcript:
CELL DIVISION IN MICROGRAVITY By Natasha Garamani, Jennifer Jiang, Jasmine Kuo, Kara Lukas, Elyssia Widjaja
Background Cell division is an integral component of life. All organisms must go through the cell cycle in order to grow, develop, and reproduce. Past experiments that have been conducted on Earth in simulated microgravity have shown that the lack of gravity causes cells to divide at a slower rate.
Purpose To discover the effect of microgravity on cell division To compare the rates of cell division in the absence and presence of gravity
If cells are exposed to microgravity during their life cycles, then there will be a reduction in cell division because of disorganization of microtubules change in actin filaments chromatin less dense increased apoptosis Hypothesis
Procedures - Ground Experiment 1. Preparation of flasks Coat inside of flasks with 3 mL of poly-lysine solution for 5 minutes Aspirate the solution and let it dry overnight Transfer 500 fibroblast cells into the flask with a pipette Add 5 mL of EMEM with 1% FBS Incubate cells overnight
Procedures - Ground Experiment 2. Initiation of Experiment Add 6.3 EMEM mixed with 1% FBS to both flasks Keep one flask in the incubator and put the other flask into a controlled room temperature environment Allow the cells to continue to grow and divide for 12 days
Procedures - Ground Experiment 3. Counting the Cells Wash the cells with 2 ml of Phosphate-Buffered Saline solution Add 0.5 ml trypsin/EDTA to cause disassociation of the cells from the flask and rotate for 1 minute. Allow the cells to detach and add 1 ml of PBS.
Procedures 3. Counting the Cells (continued) Remove medium from the flask, place it in a 15 ml centrifuge tube, and spin for 5 minutes in a tabletop centrifuge to pellet the cells. Resuspend the pellet in 0.5 ml of PBS Stain culture sample with trypan blue Count the cells collected from culture using a hemocytometer.
Conclusion Growth is more prominent for cells in incubator (at 37 degrees Celsius)
Acknowledgements Mr. Wyeth Collo for his guidance as our advisor. Dr. Susan Kane and Ms. Erin Denny of City of Hope Cancer Center, Developmental Cancer Therapeutics Program, Comprehensive Cancer Center for providing the lab space and materials. Smithsonian Air and Space Museum for hosting this event. SSEP for this invaluable experience.