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UNIVERSITY OF COLORADO COLORADO SPRINGS A & P Lab Safety Global Hazard System University of Colorado Colorado Springs Division of Environmental Health.

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Presentation on theme: "UNIVERSITY OF COLORADO COLORADO SPRINGS A & P Lab Safety Global Hazard System University of Colorado Colorado Springs Division of Environmental Health."— Presentation transcript:

1 UNIVERSITY OF COLORADO COLORADO SPRINGS A & P Lab Safety Global Hazard System University of Colorado Colorado Springs Division of Environmental Health and Safety

2 2 Overview This training consists of four modules: Module 1: Background and Overview Module 2: Chemical Safety Module 3: Waste Management Module 4: Other Laboratory Hazards

3 3 There are four primary regulations which govern how UCCS manages hazardous materials on campus. OSHA Hazard Communication (Right-to-know) – passed in 1983 significantly updated 2012 OSHA Laboratory Standard – passed in 1990 Resource Conservation and Recovery Act (RCRA) – originally passed in 1976 Colorado Springs Fire Department Background Module 1: Background and Overview

4 Live Green UCCS’s Compliance Method  Campus-wide Laboratory Safety Manual  Maintained by EHS – available online  Laboratory Registration  Updated annually by PI’s, lab managers  Laboratory Chemical Inventories  Updated annually by PI’s, lab managers  Laboratory Specific Safety Plans  Prepared by PI’s, lab managers – reviewed at least annually or whenever processes change  Comprehensive Training Programs  Provided annually and on-line UCCS has adopted a comprehensive approach to meeting these requirements. The elements incorporated in the comprehensive approach are: 4

5 5 1.Understand the updated Hazard Communication Standard 2.Understand the Safety Data Sheet 3.Understand Labels Pictograms Signal Words Hazard Statements Precautionary Statements 4.Understand the relationship of SDS and label 5.Understand storage requirements 6.Understand emergency response for chemicals Objectives Module 2: Chemical Safety

6 Understanding the GHS Labels 6 Signal word: One word used to indicate the relative severity of hazard and alert the reader to a potential hazard on the label and safety data sheet. The GHS includes two signal words:  “Warning” for less severe hazard categories and;  “Danger” for more severe hazard categories. Hazard statement(s): Phrase assigned to each hazard category that describes the nature of the hazard. Examples of hazard statements are: “Harmful if swallowed,” “Highly flammable liquid and vapor” and “Harmful to aquatic life.” Product identifiers: Names or numbers used on a hazardous product label or in a safety data sheet. They provide a unique means by which the product user can identify the chemical substance or mixture.

7 . Understanding the GHS Labels 7 Other core information to be provided: Supplier identification: Under the GHS supplier identification would include the name, address and telephone number of the manufacturer or supplier of the substance. Precautionary statement(s): Phrases that describe recommended measures that should be taken to minimize or prevent adverse effects resulting from exposure to a hazardous product, or improper storage or handling of a hazardous product. These phrases cover prevention, response, storage, and disposal of products Pictogram(s): A symbol inside a diamond with a red border, denoting a particular hazard class (e.g., acute toxicity/lethality, skin irritation/corrosion, etc.).hazard class

8 8 GHS Hazard Classification Defined criteria are used to assign a hazard classification –Physical Hazards 16 categories –Health Hazards 10 categories –Environmental Hazards 2 categories Understanding the GHS Labels

9 9 Video: https://www.youtube.com/watch?v=8queMM7VVfw&i ndex=4&list=PL4qaj9envIYnBaQSPpcOMUqWiQUAg PoMq Video 4

10 10 Understanding the GHS Labels These are the pictograms used in the GHS, with the hazard classes they are applied to. The first two rows were taken from the international transport system.

11 11 GHS vs NFPA Warning Labels We will still be utilizing the NFPA labels across campus. There are however some conflicts between the GHS and NFPA labeling systems. Namely the hazard ratings are in reverse order. GHS hazard ratings; however, will only appear on the SDS and not on the label.

12 Live Green The NFPA label is divided into four quadrants. Each quadrant describes a specific hazard: Blue = health Red = flammability Yellow = reactivity White = special hazards NFPA Warning Labels 12

13 16 sections specified in a given order of information Information in the beginning sections have a broad audience More detailed, technical information included in following sections Safety Data Sheets 13 GHS calls for a reworking of the Material Safety Data Sheets (now just Safety Data Sheets)

14 14 Safety Data Sheets

15 15 If someone breathes in a chemical: Remove the person to fresh air Stay with them until you are sure they are ok If symptoms persist, call 911 or campus police Report the incident to your supervisor If someone ingests a chemical: Remove the person from the area Call 911 or campus police Have someone locate the SDS for instructions Report the incident to your supervisor If someone gets a chemical on their skin/eyes: Immediately flush for 15 minutes with cold water at an eye wash station or shower as appropriate Always remove contacts immediately after contamination Seek medical attention if symptoms develop (rash or hives are typical symptoms) Call 911 or campus police Seek medical treatment Report the incident to your supervisor

16 Accidental Release Measures 16 Determine if the spill is Incidental Spill or Emergency Response Spill

17 Incidental Spill A spill you can handle on your own (or with the help of a coworker) Accidental Release Measures 17

18 Incidental Spill How do you know if you can handle the spill? Ask yourself if you: Have the right kind of spill cleanup materials. Have the proper gloves, goggles and other protective equipment (i.e.: apron, face shield). Have no exposure risk because it is a low toxicity chemical. Have no one with a chemical exposure or injury. Have experience or training in cleaning up this type of spill. Have a spill that will not go down the drain. Have spilled less than a gallon, minimizing the fire and exposure risks. Accidental Release Measures 18

19 Incidental Spill Cleanup How do you clean up an Incidental Spill? Follow the 4W Procedure: Accidental Release Measures 19 WrapWearWipeWarn

20 An Incidental spill becomes an Emergency Response spill if you: Do not have the proper spill cleanup materials. Do not know how to safely clean it up. Do not have your PPE (gloves, goggles, lab coat) to clean it up. Have to clean up more than a gallon of a toxic or volatile liquid. If any of these factors come into play and you cannot safely clean up your spill, and you should implement the Emergency Response spill procedure, described on the next page. Accidental Release Measures 20

21 Emergency Spill Response If you have a chemical spill that is too large to handle on your own, or one in which you do not have the proper cleanup equipment or PPE, it is an Emergency Response spill and you should follow these 4 steps: 1.Warn others and evacuate the area. If there is a fire, pull the fire alarm. 2.Secure the area: close the door, use caution tape, a sign, or post an employee (at a safe distance) to warn others not to enter.* 3.Report the spill from a safe location. (Call the UCCS Police at 911 or x3111. Give your name, call back phone number, building name, location of release, and the name and quantity of the chemical released. 4.Wait for the emergency responders from Environmental Health and Safety to provide them spill details. *Important If there is a fire or large-scale release of toxic or flammable gases, pull the fire alarm and evacuate all building occupants. Give verbal instructions to those in the immediate area to ensure they do not evacuate through the affected area of the chemical release. Accidental Release Measures 21

22 General accidents/near misses Accident Report Form in labs To be filled out by instructor Even minor accidents need form Any time a person is exposed and/or there is a significant quantity of hazardous materials involved – the report should be forwarded to EHS Emergency Preparedness 22

23 Safety Data Sheets Storage and Handling https://www.youtube.com/watch?v=uiB8qnl ZTAM&index=5&list=PL4qaj9envIYnBaQS PpcOMUqWiQUAgPoMq Video 5

24 Generic Protocols for handling chemicals Wear proper PPE No eating, drinking, smoking in lab Confine long hair & loose clothing Wear appropriate clothing (lab coat, no shorts or sandals) No bare arms, legs or midsections No working alone in labs Wash hands before leaving the lab Use a fume hood when necessary Chemical Handling 24

25 General Protocols - continued Know Location of: Fire Alarm Pull Station, Eyewashes/ Showers, Fire Extinguishers, Exits, SDSs, Spill Equipment Chemical Handling 25

26 Safety Data Sheets 26 Hand Protection – gloves – pick the right one for the job and the chemicals or hazards in question Eye Protection – goggles – glasses – face shield – use the right one for the job Hearing Protection – ear plugs – muffs Foot Protection – closed toe, closed heel, non-skid Body Protection – pants, shirts, leggings, etc. https://www.youtube.com/watch?v=GjAD83B4Ja Y&list=PL4qaj9envIYnBaQSPpcOMUqWiQUAg PoMq&index=1 Video 1

27 27 https://www.youtube.com/watch?v=e7VkIui T1kU&list=PL4qaj9envIYnBaQSPpcOMUq WiQUAgPoMq&index=3 Video 3

28 Module 3: Waste Management After completing this module you will be able to: 1.Identify who is responsible for managing chemical waste. 2.Determine if a chemical is a “regulated” hazardous waste. 3.Identify hazardous waste types by using the CRIT (Corrosive, Reactive, Ignitable, Toxic) waste characteristics. 4.Explain proper management of various waste types, including listed waste (U, P, and F listed); universal waste (batteries, lights, computers); research chemical waste (drugs, medicine, etc.); reagent containers, and scrap glass. Objectives 28

29 Live Green History Resource Conservation and Recovery Act (RCRA) – originally passed in 1976 –40 CFR Deals with waste management Requires cradle to grave responsibility 29

30 What type of hazardous waste is it? Characteristic waste Corrosive Reactive Ignitable Toxic F-Listed P-Listed U-Listed Waste Management Remember: Unwanted or broken equipment that contains hazardous chemicals or materials (such as lead, mercury, or acid) is also considered a hazardous waste. 30

31 Universal Wastes Universal wastes are regulated hazardous wastes that we might not recognize as "chemical" waste. Examples include: batteries, (recycle buckets around campus) fluorescent lights including CFL, (Facilities collects these) electronic circuit boards, (Facilities collects these) surplus computers, (Facilities collects these) aerosol cans, and (contact EHS for guidance) switches and devices containing mercury. (contact EHS for guidance) Other equipment may also contain hazardous materials, including lasers and carbon containing products. Waste Management 31 These items CANNOT be thrown in the regular trash

32 RCRA hazardous waste regulations apply to:  Spent chemicals that were "used" for their intended purpose  Unused chemicals that have expired  Chemicals that are no longer useable such as those that have become unstable and cannot be used safely  Unneeded surplus  Waste drugs, products, and equipment that contain hazardous chemicals  The RCRA rules do not apply to unused chemicals that are being stored for legitimate uses. However, you may not store materials indefinitely with no legitimate use intended. In other words, you may not speculatively accumulate chemicals. If you know your materials are waste, the next step is to determine the type of waste you have, which brings us to the CRIT waste criteria. Waste Management 32

33 Collection of Hazardous Waste To manage your experiments' chemical wastes, follow this five-step procedure: 1.Collect the waste in an empty chemical reagent bottle which is chemically compatible and has no cracks, dents, or rust. Waste containers should be provided with secondary containment; 2.Seal the container with its original lid. Do not use corks, rubber stoppers, or Parafilm. Keep containers closed at all times except when adding or removing wastes; 3.Complete a Hazardous Waste Label and place it over the existing reagent label as soon as the first drop of waste is added to the container; 4.Keep the chemical waste container within eyesight at all times, otherwise you must place the container inside a locked room or a locked storage cabinet, and 5.Inspect the waste container weekly for leaks, and document your inspection in your SAA log. Waste Management 33

34 Other Chemicals Many of our research chemicals are not regulated as hazardous waste, but still must be collected for proper disposal. Examples of these chemicals are: visualizing dyes (ethidium bromide), Drug Enforcement Administration (DEA) controlled substances, expired pharmaceuticals, copper sulfate, and dimethyl sulfoxide. As a general rule, all research chemicals must be properly collected and disposed of through Environmental Health and Safety. Waste Management 34

35 What cannot be disposed of in the trash? Biohazard bags not autoclaved Broken glass Chemicals Mercury thermometer Spill clean-up debris Waste Management 35

36 Hazards Up to this point we have discussed hazardous materials management and waste management. Besides hazardous materials/waste there are a number of other types of hazards present in the laboratories. We will now take a few minutes to quickly review the management practices associated with some of these other hazards. 36 Module 4: Other Hazards

37 Types of Hazards Mechanical High Energy Lasers Magnetic Field Generators Physical Hazards Compressed Gases Power Tools Noise You need to review the lab operations with your Principal Investigator (PI) to determine which of these hazards may be present in your lab. Once you identify the hazards – you then need to review appropriate safety precautions for each hazard. 37 https://www.youtube.com/watch?v=q8UiamEWz4Q&list=PL4q aj9envIYnBaQSPpcOMUqWiQUAgPoMq&index=6 Video 6

38 Biological Hazards Biological hazards are substances or agents (e.g., viruses, bacteria, spores, fungi, bloodborne pathogens, prions, or toxins from a biological source) that pose a threat to the health of living organisms. Biological hazards can include human bodily fluids and tissues. Biological Safety 38

39 Biological Safety Infectious Hazards Approval from Institutional Biosafety Committee (IBC) Use of Lysol/Bleach to disinfect Use of biological cabinet Use of autoclave to sterilize Use of PPE Dissections Biological Safety 39

40 Bloodborne Pathogens Take on-line training at the EHS website BBP Exposure Control Plan Wear proper PPE when exposure to bodily fluids is likely Bloodborne Pathogens 40

41 Biological Safety Varying degrees of risk are associated with exposure or infection. For infectious agents, these risks are categorized by "risk groups" (RG) based on the potential effect on a healthy adult human, taking many factors into account, such as pathogenicity, mode of transportation, and the availability of effective treatments or preventive measures. 41

42 Biological Safety Risk GroupDescriptionExample Organism Risk Group 1Agents that are not associated with disease in healthy adult humans. B. subtilis, S. cerevisiae (Baker's yeast) Risk Group 2Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available. S. aureus, G. lamblia, Adenovirus Risk Group 3Agents that are associated with serious or lethal human disease for which preventive or therapeutic interventions may be available (high individual risk but low community risk). B. pseudomallei, Hantaviruses Risk Group 4Agents that are likely to cause serious or lethal human disease for which preventive or therapeutic interventions are not usually available (high individual risk and high community risk). Herpes B Virus, Ebola, Marburg Risk Groups 42

43 Anatomy & Physiology Specifics

44 EMBALMING CHEMICALS Formaldehyde Phenol Methanol Glycerin

45 Formaldehyde 2% Potential human carcinogen PEL – 1 ppm Odor Threshold – 0.8 ppm Irritation to eyes, nose and throat - above 0.1 ppm Skin contact – sensitization

46 Phenol 10% Irritations and burns Systemic toxicity Sweet acrid odor PEL 5 ppm Odor Threshold 1 ppm Skin contact is major route of exposure

47 Methanol 11% Irritations to the skin, eyes, nose, throat, nervous system Pungent odor PEL 200 ppm

48 Glycerin 11% Irritations to the eyes, nose, throat, respiratory system colorless, odorless PEL 5 ppm

49 Infectious Agents Embalming fluids destroy many of the infectious agents

50 Protection - general Nitrile gloves – may need to change Leg covering – ¾ length Hand washing No eating or drinking Cross-contamination

51 Protection – dissection Nitrile gloves – change at least every 1.5 hours Lab coats Eye protection Contact lenses (not recommended) Use scalpel blade removers Place sharps in designated container

52 Ergonomics Get close to the area with which you are working Bend at the knees Avoid excessive repetitive motions Avoid extensive fixed positions Take regular breaks

53 Primary responsibility for safety in the lab lies with every individual PI’s and lab instructors are responsible for enforcing guidelines in the labs Safety should become an integral part of our daily activities Conclusion 53


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