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CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL CERTIFICATION Simultaneous quantitative determination of several components.

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Presentation on theme: "CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL CERTIFICATION Simultaneous quantitative determination of several components."— Presentation transcript:

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2 CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL

3 CAPILLARY ELECTROPHORESIS SYSTEMS CAPEL CERTIFICATION Simultaneous quantitative determination of several components in a complex mixture

4 ADVANTAGES CAPEL-SERIES  High separation efficiency  Low reagent consumption  Simple sample preparation  Compact benchtop design  Autosamplers (10 inlet and and 10 outlet vials) in CAPEL-104, CAPEL-104T, and CAPEL-105  Implementation of various methods (Capillary Zone Electrophoresis, Micellar electrokinetic chromatography, Capillaryisoelectric focusing, capillary gel electrophoresis, capillary isotachophoresis)

5 ELECTROOSMOTIC FLOW (EOF) R – Si-OH  R – Si-O - + H + K+K+ A-A- EOF + _ pH > 3.0 Both anions and cations can be analysed in one run                

6 BASIC FEATURES CAPEL-103Р, 103РТ CAPEL-104, 104Т CAPEL-105 wavelength 254 nm autosampler liquid cooling (model 104Т) wavelength 254 nm manual operation mode liquid cooling (model 103РТ) wavelength nm autosampler liquid cooling

7 MAJOR APPLICATIONS  pesticides  inorganic ions  phenols Environment Pharmacology  medicines formulations  pharmacokinetics Food  preservatives  dyes  target compounds, etc. Clinical diagnostics  proteomics  DNA / RNA  affinity studies  immunoanalyses Forensic Investigations  drugs  explosives  lethal markers, etc. Chemical industry

8 ANALYSIS OF PSYCHOTROPIC DRUGS MECK separation of carbamazepine and its main metabolites Chiral separation of carbamazepine metabolite

9 ANALYSIS OF ANTIEPILEPTIC DRUGS Microemulsion EKC of 6 closely related antiepileptic drugs Selectivity changes are seen with different organic modifiers

10 min mAU ANALYSIS OF WATER – SOLUBLE VITAMINS Buffer0.01M borate, pH 9.8 Samplestandard solution of vitamins Capillaryl/L 50/60 cm, id 75 µm Injection600 mbar * s Voltage+20 kV Detection 254 nm 1 – vitamin В 1 2 – vitamin В 12 3 – vitamin В 2 4 – vitamin В 6 5 – ascorbic acid 6 – nicotinic acid 7 – folic acid

11 ANALYSIS OF MEDICALLY RELEVANT PEPTIDES ANALYSIS OF MEDICALLY RELEVANT PEPTIDES 1-antithrombine 2-bradikinine 3-angiotensin I 4-angiotensine II Buffer 10 mM phosphate, pH 2.5 Capillary 26 / 35 cm, 50µm ID Injection 150 mbar x sec Voltage25 kV Sample 10 µg/mL each

12 ANALYSIS OF THE CATION COMPOSITION OF THE PORTABLE, NATURAL AND WASTE WATER Buffer6 mmol benzimidazole, 2.5 mmol tartaric acid, 2.0 mmol 18-crown-6 Samplecalibration solution Capillaryl/L 50/60 cm, id 75 µm Injection150 mbar * s Voltage+10 kV Detection254 nm, indirect 1 – cesium 2 – ammonium 3 – potassium 4 – sodium 5 – magnesium 6 – lithium 7 – strontium 8 – barium 9 – calcium            

13 ANALYSIS OF THE ANION COMPOSITION OF THE PORTABLE, NATURAL AND WASTE WATER Buffer5 mmol chromate, 20 mmol DEA, 1.65 mmol CTAB Samplecalibration solution, 5 mg/l of each anion (fluoride  2.5 mg/l) Capillaryl/L 50/ 60 cm, id 75 µm Injection300 mbar * s Voltage  17 kV Detection254 nm, indirect 1  chloride 2  nitrite 3  sulfate 4  nitrate 5  fluoride 6  phosphate *  bromide hollow **  hydrocarbonate           

14 ANALYSIS OF PESTICIDES OF SYMMETRIC TRIAZINE GROUP Buffer10 mmol sodium borate, 32 mmol SDS Sampletest solution, 2.5 mg/l of each compound Capillaryl/L 60/70 cm, id 75 µm Injection900 mbar * s Voltage+20 kV Detection229 nm 1 – simazine 2 – atrazine 3 – propazine 4 – prometryne       

15                ANALYSIS OF SYNTHETIC DYES Buffer5 mmol phosphate, 5 mmol hydrocarbonate, pH 10.5 Samplestandard mixture of dyes Capillaryl/L 50/60 cm, id 75 µm Injection600 mbar*s Voltage+20 kV Detection254 nm 1 – Rhodamine B (C.I ) 2 – Patent Blue V (E131, C.I ) 3 – Orange I (C.I ) 4 – Acid Green S (E142, C.I ) 5 – Acid Violet 5BN (C.I ) 6 – Quinoline Yellow (E104, C.I ) 7 – Sunset Yellow FCF (E110, C.I ) 8 – Tartrazine (E102, C.I ) 9 – Azorubine (E122, C.I ) 10 – Amaranth (E123, C.I ) 11 – Chrysoine (C.I ) 12 – Brilliant black PN (E151, C.I ) 13 – Indigotin (E132, C.I ) 14 – Brilliant Blue FCF (E133, C.I )

16 ANALYSIS OF BENZOIC ACID DERIVATIVES Buffer 25 mM borate pH 9.18 Capillary 26 / 35 cm, 50µm ID Injection 150 mbar x sec Voltage 25 kV

17 APPLICATIONS  Determination of inorganic anions in water samples Determination of inorganic anions in water samples  Determination of inorganic cations in water samples Determination of inorganic cations in water samples  Determination of phenoxycarboxylic acid herbicides in water Determination of phenoxycarboxylic acid herbicides in water  Determination of caffeine and preservatives in beverages Determination of caffeine and preservatives in beverages

18 SPECIFICATION Capel-103Capel-104Capel-105 Detector Line-source lamp, 254 nm Light source - deuterium lamp 190 – 380 nm Operating mode Manual Fully automated, programmable Capillary cooling Air-forced with ambient t°C In ver.(T) – thermostatic liquid cooling with pre-set t°C Liquid cooling with thermostabilisation, from -10 up to +30°C with respect to ambient Sampler Manually operated for 4 vials Autosampler, 10 inlet, 10 outlet vials

19 CONTACTS LUMEX LTD. 19, Moskovskiy pr., St. Petersburg, Russia Phone: +7 (812) , +7 (812) Fax: +7 (812) LUMEX INTERNATIONAL 4736 Lee Hwy, suite 203, Arlington VA 22207, USA Phone/Fax: +1 (703) ; OhioLumex Co. 9263, Ravenna Rd., Unit A3 Twinsburg, OH USA Phone: +1 (888) ; +1 (330) Fax: +1 (330)


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