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Shocking: Elimination of S. Aureus Biofilm from Stainless Steel by Electrolysis Matthew J. Dietz, MD; Jacob Conjeski MD; Vincent L. Kish AS; Brian R.

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Presentation on theme: "Shocking: Elimination of S. Aureus Biofilm from Stainless Steel by Electrolysis Matthew J. Dietz, MD; Jacob Conjeski MD; Vincent L. Kish AS; Brian R."— Presentation transcript:

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2 Shocking: Elimination of S. Aureus Biofilm from Stainless Steel by Electrolysis Matthew J. Dietz, MD; Jacob Conjeski MD; Vincent L. Kish AS; Brian R. Hamlin, MD West Virginia University Department of Orthopaedics

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4 Orthopaedic Biofilm Orthopaedic Biofilm Adherent Bacteria Adherent Bacteria EPS (extra-cellular polysaccharide) EPS (extra-cellular polysaccharide) Polymicrobial Polymicrobial

5 S. Aureus S. Epi EPS Implant Spork

6 “race for the surface” -- Gristina, A. “Biomaterial-Centered Infection: Microbial Adhesion Versus Tissue Integration. Science. Vol. 237, No (Sep. 25, 1987), pp Bacterial Colonization vs. Tissue Integration Bacterial Colonization vs. Tissue Integration x Antibioticsto Eliminate Biofilm

7 “Strategerie”

8 Debridement with Retention Staged Explant/Exchange Resection Arthroplasty ArthrodesisAmputation

9 Hypothesis

10 S. aureus S. aureusBIOFILM

11 Biofilm and WVU Orthopaedics Create Biofilm --Goeres et. al. “Statistical assessment of a laboratory method for growing biofilms.” Microbiology. 2005; 151:

12 Where’s the Biofilm?

13 -- Rabinovitch, C et al. “Removal and Inactivation of Staphylococcus epidermidis Biofilms by Electrolysis.” App and Enviromental Microbiology. Sept 2006:

14 Control1 Volts Avg % reduction in CFU’s/cm2 Anode (n=5) (n=5) Cathode Avg % reduction in CFU’s/cm2

15 Biofilm and WVU Orthopaedics

16 Control 4 V 2 V 1 V 0.5 V

17 However…

18 Conclusions Need for adjuntive measures Connection to Cathode for S. aureus CFU reduction independent of voltage 98% of CFU’s via electrolysis 98% of CFU’s via electrolysis

19 To Do List…  Biofilm Models  Detection of Biofilm  Electrolysis  Various Metals  In-vivo  Vary biofilm makeup  Means of Removal

20 The War Continues… Thank You


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