Cutoff on RRS and Translation Efficiency Provide Better Separation
RSS separates coding regions This is using open reading frames (ORFs) in each of these regions.
There are lincRNAs with high conservation and a high RSS score (do these code proteins?)
Why high TE for lincRNAs? Reasons the authors give: – Non-ribosomal contamination – Seductive hypothesis in the discussion: Translational machinery is always engaged with transcripts in the cytosol of the cell. “Pervasive” engagement of the ribosome with transcripts. – This can be co-opted by evolution to create new protein- coding genes.