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Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha Fertilization – transformation of meiotic cells into mitotic cells  Gametes – terminally differentiated.

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Presentation on theme: "Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha Fertilization – transformation of meiotic cells into mitotic cells  Gametes – terminally differentiated."— Presentation transcript:

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2 Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha

3 Fertilization – transformation of meiotic cells into mitotic cells  Gametes – terminally differentiated and highly specialized  Genomes - (oocyte, sperm) transcriptionally inactive after fertilization One cell stage embryo is totipotent Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES

4 A)Sheep B)Mouse C)Human D)Rabbit E)Bovine F) Pig G) Monkey

5 Mouse embryo Human embryo PB MPN FPN MPN 5-MeC

6 Human embryo

7 PB PN MPN FPN 49% of human 1-cell stage embryos showed abnormal demethylation pattern

8 2PB FP MP

9 Wilmut, I et al. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385,

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11 A B AB

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15 Enukleace

16 Přenos jádra

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23 What do we need??

24 Oct4Cdx2 DAPI merge

25 Nanog

26 Oct4DAPI

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29 Differentiation βIII tubulin TROMA 1 DAPI MF20 + EB formation

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35 Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň

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37 Pacient ESc linie

38 Producing primate embryonic stem cells by somatic cell nuclear transfer Nature - doi: /nature06357 …. potřebujeme vůbec přenosy jader?

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44 Děkuji za pozornost


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