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Abundance and structure of microorganisms related to methane cycling in five European peatlands: Influence of plant cover and restoration stage (WP1) A.

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Presentation on theme: "Abundance and structure of microorganisms related to methane cycling in five European peatlands: Influence of plant cover and restoration stage (WP1) A."— Presentation transcript:

1 Abundance and structure of microorganisms related to methane cycling in five European peatlands: Influence of plant cover and restoration stage (WP1) A. Gattinger et al. Technical University of Munich (at the campus of GSF-Research Center for Environment & Health) Chair of Soil Ecology D Neuherberg

2 I. Depth distribution of archaeal (methanogenic) biomass among countries Country x Depth i20:0 I20:1 I40:0 i40:1cyx i40:1cy i40:2cyx i40:2cy Finland France/B France/LScotland Switzerland 0,00 20,00 40,00 60,00 80,00 100,00 PLEL_(nmol/g dm) 329 Depth Finland France/B France/LScotland Switzerland Pooled for „country“

3 I. Depth distribution of methanotrophic biomass (Type I and II) among countries Finland France/B France/L ScotlandSwitzerland PLFA (nmol/g dry matter) Country x Depth 0,00 20,00 40,00 60,00 80,00 100,00 120,00 140,00 Depth Finland France/B France/LScotland Switzerland Country 0,00 5,00 10,00 15,00 Type I Type II Finland France/B France/LScotland Switzerland 15,00 Pooled for „country“ Country x Depth

4 II. Methanogens to methanotrophs along gradients Finnland Jura sites

5 …the presented data should be combined with CH4/CO2 flux data for making system-related studies. ………………………………………………………………………………….. PLFA analyses completed (WP1) apart from specific biomarkers, data of other PLFA is avavailable (saturated, mono-unsaturated and polyunsaturated fatty acids) for calculating total biomasses of eg. Bacteria, fungi, etc.

6 Labelled litter 13 C - 15 N 15 N mineralization towards microbes Microbial communities : 13 C PLFA analysis 13 C & 15 N in microbial biomass towards peat 13 C & 15 N (K 2 SO 4 extract without fumigation) Peat column WP3: Microbial transformations of plant litter (TUM-BO, ECOBIO, EPFL/WSL) (TUM-BO, ECOBIO, EPFL/WSL)

7 What do we want to know? 1.Carbon transformation  How much plant C is consumed by the microbial biomass?  How much plant C is used by Bacteria, Archaea, Eukarya and when?  How much plant C is „somehow“ stabilised? 2. N transformation  How much plant N is consumed by the microbial biomass?  How much plant N is mineralised?  How much plant N is somehow stabilised? Labelled litter 13 C - 15 N 15 N mineralization towards microbes Microbial communities : 13 C PLFA analysis 13 C & 15 N in microbial biomass towards peat 13 C & 15 N (K 2 SO 4 extract without fumigation) Peat column

8 Simultaneous identification and quantification of PLFA/PLEL from environmental samples and their corresponding 12 C/ 13 C ratios by GC/MS-c-IRMS MS (DSQ) IRMS (DeltaPlus Advantage ) 20% of the analyte 80% of the analyte Agilent MSD

9 SATFAs (Bacteria) PUFAs (Eukarya)

10 Bacteria: Gram-positive (i15:0) ControlSphagnumEriophorum δ 13C (‰) sampling date (= 15d, 60d, 150d) Horizon 012

11 Bacteria: Gram-positive (a15:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

12 Bacteria: Gram-negative (cy17:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

13 Bacteria: Gram-negative (cy19:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

14 Archaea: Euryarchaeota (i20:0) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

15 Eukarya: Fungi (18:2d9,12) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

16 Eukarya: Protozoa (20:4d5,8,11,14) ControlSphagnumEriophorum δ 13C (‰) sampling date 123 Horizon

17 What needs to be done? 1.Carbon transformations  LC/IRMS of prepared microbial biomass C (CFE) extracts  Calculation of group-specific microbial plant C utilisation  Mass balance for the „whole“ system 2. Nitrogen transformations  EA-IRMS of prepared microbial biomass N (CFE) extracts  EA-IRMS of mineral N extracts (?)  Calculation of microbial plant N utilisation  Mass balance for the whole system

18 LC IsoLink

19 Chromatogramm Standard

20 Reproduzierbarkeit des LC/IRMS-Signals δ 13 C [‰ PDB] n = 3 + SE Neue Methode ist:  reproduzierbar stabil  sensitiv  effizient (ca. 100 Proben/Tag)


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