Transcription: Sending the message Sigma factor recognizes a specific sequence of nucleotide sequence (promoter) on a DNA strand. It is involved only in initiation. Transcription stop at terminator sequence.
DNA as the Template for RNA SynthesisDNA as the Template for RNA Synthesis DNA as the Template for RNA Synthesis RNA polymerase always reads in the 3’ to 5’-direction. One strand of DNA serves as the template or sense strand.
Conserved sequences in promoters recognized by E. coli RNA polymerase
The diauxie When exposed to glucose + lactose, E. coli does not consume lactose until glucose is exhausted, resulting in two exponential growth phases separated by a lag. This is called the diauxie or “double growth.” Diauxie occurs because synthesis of lactose permease and -galactosidase is somehow abolished in the presence of glucose. Question: What is the mechanism that suppresses synthesis of lactose enzymes? Inada et al, Genes to Cells, 1, 293, 1996 OD - galactosidase