1. Isabel Borja NILE 2011 Professional English for Clinical Microbiology Answer key Unit 1 CLIL Project

2. 2 Answer key Lesson 1.1 – Gram Stain

3. 3 Activity 1 Significance of Gram stain: Gram stain is the main stain procedure in Microbiology. It enables us to know several things about bacteria in the smear: - GRAM-STAIN REACTION - SHAPE - ARRANGEMENTS of bacteria Bacteria are divided into two large groups depending on their Gram- stain reaction: the so-called Gram-positive (GP) that remains coloured after the decolouration procedure and the Gram-negative (GN) that do not retain the dye and take the colour of the counterstain. Those differences rely on the basis of cell wall structures. Considering together Gram reaction and shape we may set the four big groups of bacteria: GP bacilli, GP cocci, GN bacilli, and GN cocci.

4. 4 Activity 2

5. 5 Activity 3

6. 6 Activity 4

7. 7 Activity 5

8. 8 Answer key Lesson 1.2 – The Microscope

9. 9 Activity 1

10. 10 EYEPIECE ARM NOSEPIECE CONDENSER DIAPHRAGM LEVER CONDENSER BASE OBJECTIVE STAGE CLIPS STAGE STAGE KNOBS COARSE FOCUS KNOB FINE FOCUS KNOB Activity 2.a

11. 11 Activity 2.b

12. 12 Activity 3

13. 13 Activity 3

14. 14 Activity 3

15. 15 Activity 4

16. 16 Activity 5 Image 5 D: urine Image 1 B: cerebrospinal fluid Image 2 C: urethral discharge Image 3 A: vaginal discharge Image 4 E: sputum

17. 17 Answer key Lesson 1.3 – Preparing Culture Media

18. 18 Activity 1

19. 19 Activity 2

20. 20 Activity 2

21. 21 Activity 3

22. 22 Activity 4

23. 23 Activity 5

24. 24 Answer key Lesson 1.4 – Bacterial Culture

25. 25 Activity 1

26. 26 Activity 2

27. 27 Activity 3 Streak plate method: Signature Initial inoculum Final spread Second spread First spread

28. 28 Activity 4

29. 29 Activity 5 Activity 5.a - Bacterial standards:

30. 30 Activity 5

31. 31