Presentation on theme: "Development of the Respiratory System and Diaphragm"— Presentation transcript:
1 Development of the Respiratory System and Diaphragm Matt Velkey454D DavisonReading: Langman’s Ch. 11 (pp ) and Ch. 13 (pp )
2 The respiratory tract is derived from foregut endoderm and associated mesoderm Lateral folding:Cranio-caudal folding:Langman’s fig 6-18Langman’s fig 6-17Parietal (aka somatic) mesoderm lines embryonic body cavity (coelom)Visceral (aka splanchnic) mesoderm covers endodermal gut tubeGut tube suspended from body wall by dorsal mesentery
3 The respiratory tract is derived from foregut endoderm and associated mesoderm From endoderm:epithelial lining of trachea, larynx, bronchi, alveoliFrom splanchnic mesoderm: cartilage, muscle, and connective tissue of tract and visceral pleura.Carlson fig 15-02
4 The lung buds form during the 4th week Langman’s fig 13.1AInitially appear as the respiratory diverticulum, which is a ventral outgrowth of foregut endodermMESODERM dependent process: Retinoic acid produced by adjacent mesoderm induces expression of TBX4 in foregut endoderm. TBX4 induces growth and differentiation of the trachea and lungs.
5 Splitting of foregut into esophagus and trachea Langman’s fig 13-02Tracheo-esophageal ridges: longitudinal ridges that eventually fuse to separate trachea from esophagus.
6 Tracheo-esophageal fistulas Larsen’s fig 11-4Langman’s fig 13-03Incomplete separation and/or atresia of trachea and esophagus (B on right shows esophageal atresia)Defect likely in mesoderm and usually associated with other defects involving mesoderm (cardiovascular malformations, VATER / VACTERL, etc.)VATER = Vertebral anomalies, Anal atresia, Tracheoesophageal fistula, Esophageal atresia, Renal atresiaVACTERL = VATER + Cardiac defects & Limb defects
7 Tracheoesophageal Fistulas / Esophageal Atresia Langman’s fig 6-18Occur in approx 1/3000 births, most (90%) are that shown in (A) above.Complications:PRENATAL: Polyhydramnios (due to inability to swallow amniotic fluid in utero)POSTNATALGastrointestinal: Infants cough and choke when swallowing because of accumulation of excessive saliva in mouth and upper respiratory tract. Milk is regurgitated immediately after feeding.Respiratory: Gastric contents may also reflux into the trachea and lungs, causing choking and often leading to pneumonitis.Surgical repair (neonatal or in utero) now result in 85% survival rates.
8 Clinical Correlation: Tracheoesophageal fistula in a male fetus with Trisomy 18 at 17 weeks. The upper esophageal segment ends blindly (pointer).Clinical Correlation:Moore & Persaud fig 10-6
9 Clinical Correlation: Tracheal agenesis:Lungs bud off esophagusUniversity of Michigan Pathology Dept.
10 Successive stages in the development of the larynx: The epithelial lining of the larynx is of endodermal origin, which proliferates and temporarily OCCLUDES the lumen of the larynx. A combination of apoptosis and growth of the wall of the larynx allows recanalization by about 10 weeks.The cartilages and muscles of the larynx arise from mesenchyme from the 4th and 6th pharyngeal arches and are innervated by branches of the vagus nerves (4th arch by the superior laryngeal branch, 6th arch by the recurrent laryngeal branch).4 weeks5 weeks6 weeks10 weeksMoore & Persaud fig 10-3
11 Clinical Correlations: Laryngeal AtresiaFailure of recanalization results in obstruction of the upper airway - congenital high airway obstruction syndrome (CHAOS). The atresia or stenosis causes lower airways to become dilated, lungs to enlarge and become echogenic and the diaphragm becomes flattened or inverted. Can be detected by ultrasound.Laryngeal WebResults from partial recanalization of the larynx during the 10th week. A membranous web forms at the level of the vocal cords, partially obstructing the airway.
12 Progressive changes in the development of the laryngotracheal tube: Endodermal lining distal to the larynx differentiates into the epithelium and glands of the trachea and pulmonary epithelium. The cartilage, connective tissue and muscles of the trachea derive from splanchnic mesenchyme.4 weeks10 weeks14 weeks - photomicrograph11 weeksMoore & Persaud fig 10-4
13 Growth of lungs into the body cavity Larsen’s fig 14-03Foregut endoderm surrounded by visceral (splanchnopleuric) mesoderm and suspended in body wall by dorsal mesenteryAs lungs grow, they expand into the body cavity
14 Differentiation of pleural membranes The lung buds “punch” into the visceral mesoderm. The mesoderm, which covers the outside of the lung, develops into the visceral pleura. The somatic mesoderm, covering the body wall from the inside, becomes the parietal pleura. The space between is the pleural cavity.Left: Fig 13.6ARight: Fig 13.7Langman’s fig 13-06Langman’s fig 13-07
15 Pleuropericardial folds separate pleural and pericardial cavities. 6 weeks - pleuropericardial membrane reaches midline5 weeks - pleuropericardial fold formsMoore & Persaud fig 10-47 weeks -further maturation of pericardium (expands pleural cavity8 weeks - lungs grow and expand into pleural cavity
16 Separating the abdominal and thoracic cavities: development of the septum transversum and diaphragmAs the embryo folds, a connective tissue structure, the septum transversum forms between the heart and body stalk.Larsen’s fig 4-01
17 Separating the abdominal and thoracic cavities: development of the septum transversum and diaphragm Extension of the septum transversum partially divides abdominal and thoracic cavitiesGrows in a roughly transverse plane from front to backIs initially at the level of C1, but is displaced caudally by differential growth of the embryoAt weeks 5-6, myoblasts migrate into septum, carrying innervation with them (ventral rami from C3, 4, and 5) –hence, the course of the phrenic nerveBy week 8, is angled downward such that front of septum is at about T7, back edge is at about T12 (similar to adult)Carlson fig 15-32
18 Separating the abdominal and thoracic cavities: development of the septum transversum and diaphragm Larsen’s fig 11-10Larsen’s fig 11-09The septum transversum stops at the gut tube, leaving two open passageways on the left and right sides, aka the “pericardioperitoneal canals” (aka pleural canals, shown on the left)Closing off these canals requires growth from the dorsolateral body wall, aka the “pleuroperitoneal membranes” (shown on the right)Defects in this process cause CDH (congenital diaphragmatic hernias): abdominal contents herniate into pleural cavities and interfere with lung development.
19 Congenital Diaphragmatic Hernias Relatively common (1/2000 births)Hiatal hernias are most frequent, but effects are rather minor due to small size of defectHernias due to failure of one or both pleuroperitoneal membranes to close off pericardioperitoneal canals have much more significant clinical impact because herniated abdominal contents interfere with lung development.80-90% of hernias with clinical impact are on the left side. Large defects have high mortality due to extent of lung hypoplasia and dysfunction.Langman’s fig 11-09
20 Initial Patterning of the Lung: First three branching events are stereotyped: Trachea into two primary bronchi (left and right)Left primary bronchus into two secondary bronchi (corresponding to the two lobes of the left lung)Three secondary buds form on the right (corresponding to the 3 lobes on the right)Ten tertiary (segmental) bronchi form in the right lung andEight bronchi form in the left lung - establishing the 18 brochopulmonary segments of the adult human lung.Carlson fig 15-25(10) Segmental bronchi (7-8)
21 Development of the human lung (note: panel 1 is as viewed from the front, panels 2 & 3 are as viewed from the back)RLLRLRArmed Forces Institute of Pathology7=trachea6=right main bronchus12=R middle lobe primordium13=R superior lobe primordium11=R inferior lobe primordium1=left main bronchu10=L superior lobe primordium9=L inferior lobe primordium7=trachea3=left main bronchus5=L superior lobe1=L inferior lobe6=R superior lobe4=R middle lobe2=R inferior lobe5=L superior lobe1=L inferior lobe6=R superior lobe4=R middle lobe2=R inferior lobe
22 Endodermal/Mesenchymal Interactions Important for Branching MorphogenesisDissected embryonic mouse lung:Right side cultured unperturbedafter dissection (i.e. covered by lung mesenchyme).Left bronchial tip covered withtracheal mesenchyme.Note no branching occurs atleft bronchial tip due totracheal mesenchymeinhibition.Gilbert fig 15.31
23 Signaling molecules known to be important for lung budding and branching morphogenesis Tbx / RA signaling induces mesenchyme to secrete FGF10, which induces epithelial growth.Branching initiated by BMP4 secretion of apical epithelial cells (this arrests their proliferation). Epithelium also secretes Shh, which inhibits mesenchyme proliferation and FGF10 secretion. Mesenchymal cells secrete TGFβ which promotes deposition of ECM.FGF10 signaling NOT inhibited at lateral aspects of both, promoting growth on either side.Fig from Carlson (2009). Human Embryology and Developmental Biology, 4th ed.Carlson fig 15-26
24 By the end of the sixth month, 17 generations of subdivisions have formed. Six more divisions occur during postnatal life for a total of 23 branching events in the adult human lung.Branching continues to be regulated by epithelial-mesenchymal interactions (deriving from endodermal epithelial lung buds and the splanchnic mesoderm surrounding them).MAIN POINT: Branching morphogenesis in the lungs is mesoderm and retinoid-dependent (among other factors). Late disruption may have minor effects whereas early disruption may result in hypoplasia or even agenesis.
25 Stages of Maturation of the Lungs Canalicular Period (16-26 weeks):Bronchi, terminal bronchioles become larger, lung tissue becomes highly vascular. Surfactant production begins around week 22, but not enough to prevent airway collapse (atelacstasis). Alveolar ducts with terminal sacs form by week 24, so limited respiration is possible.Pseudoglandular Period (5-17 weeks):By 17 weeks, all major elements have formed, except those involved with gas exchange (fetuses unable to survive if born at this stage).Terminal Sac Period (26 weeks to birth):Many more terminal sacs develop, with very thin epithelium and capillaries bulging into the developing alveoli. Blood-air barrier becomes well-developed. Surfactant production is sufficient to prevent atelacstasis.Alveolar Period (late fetal period to age 8):Alveoli-like structures are present by 32 weeks. Epithelial lining of sacs attenuate to extremely thin squamous epithelia, capable of gas exchange. 95% of characteristic, mature alveoli develop after birth.Moore & Persaud fig 10-9
26 Development of lung tissue involved in air exchange Langman’s figs & 09Canalicular Period:(16th-26th week)Terminal Sac Period:(24th weeks to birth)Type I squamous cellsAlveolar Period:(late fetal thru childhood, Type II, surfactant-producing cells)
27 At birth:Alveoli continue to mature after birth, become more muscular. Growth of lungs after birth due primarily to increase of respiratory bronchioles and alveoli. Only 1/6 of adult alveoli present at birth.Lungs are fluid filled; fluid squeezed out and into lymphatics and blood vessels, expelled via trachea at delivery.Surfactant remains on surface, lowers air/blood tension.
28 Surfactant proteins augment function of phospholipid surfactants Gilbert fig 15.32Four major surfactant proteins: A, B, C, and DSurfactant A: activates macrophages to elicit uterine contractions, also important in host defenseSurfactant B: organizes into tubular structures that are much more efficient at reducing surface tension (specific deficiency in Surfactant B can lead to respiratory distress)Surfactant C: enhances function of surfactant phospholipidsSurfactant D: important in host defense.
29 Clinical Correlation: Respiratory Distress Syndrome/Hyaline Membrane Disease:This disease affects 2% of live newborn infants, with prematurely born being most susceptible. 30% of all neonatal disease results from HMD or its complications.Carlson fig 15-30Surfactant deficiency is the major cause of RDS or HMD. The lungs are underinflated and the alveoli contain a fluid of high protein content, probably derived from circulation substances and injured pulmonary epithelium.In addition to prematurity, prolonged intrauterine asphyxia may produce irreversible changes in Type II alveolar cells, rendering them incapable of producing surfactant. Other factors may contribute to surfactant deficiency, but the genetics of surfactant production are not well-defined.Prolonged, labored breathing damages alveolar epithelium, leading to protein deposition, or “hyaline” changes (shown in figure).
30 Clinical Correlations: Congenital Lung Cysts:Cysts (filled with fluid or air) are thought to be formed by the dilation of terminal bronchi, probably due to branching irregularities in later development. If severe, cysts are visible on radiographs. Highly variable outcomes result from different cystic conditions.Agenesis of the Lungs:Can occur bilaterally or unilaterally due to early failure of the respiratory bud to develop and/or branch (e.g. insufficient mesoderm, teratogens such as RA or alcohol, or genetic mutation). Unilateral lung agenesis is compatible with life (remaining side usually hyperexpands and compensates).Lung Hypoplasia:May be due to inadequate branching morphogenesis, but often caused by congenital diaphragmatic hernias or congenital heart disease. Characterized by reduced lung volume. Extreme hypoplasia is inconsistent with life.
31 Figures from the following texts: Bruce M. Carlson, Human Embryology & Developmental Biology, 4th ed. Scott Gilbert, Developmental Biology, 8th ed. Keith Moore & Vid Persaud, The Developing Human, 8th ed. Tom W Sadler, Langman’s Medical Embryology, 11th ed. Gary Schoenwolf, et al., Larsen’s Human Embryology, 4th ed.