We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published byKatelynn Hauff
Modified about 1 year ago
Roadmap of protein traffic inside cell
Two ways in which a sorting signal can be built into a protein
Some typical signal sequences
Nuclear Pore complexes perforate the nuclear envelope
Nuclear pore complexes
Nuclear side of the nuclear envelope
Face on view of nuclear complexes without the membrane
Side view of 2 nuclear pore complexes
Transport through nuclear pore complexes occurs through free diffusion and active transport
Nuclear import signal direst proteins to the nucleus
Single amino acid mutation in signal will prevent import into the nucleus
Nuclear import receptors bind to nuclear porins and nuclear localization signal of cargo protein Different nuclear localization signals bind different import receptors
Ran GTP provides energy for nuclear protein import
Ran-GTP controls cargo loading and unloading
Transmembrane transport into the mitochondria and chloroplasts
Subcompartments of mitochondria and chloroplasts
Signal sequence for mitochondrial import red = positively charged yellow = nonpolar
Signal sequence for mitochondrial import can form amphipathic α - helix α - helix is recognized by receptor proteins
Protein translocators in mitochondrial membrane
Protein import by mitochondria
DVD Clip 55
Protein transport into the peroxisomes
Roadmap of protein traffic inside cell
Insertion of rhodopsin into the ER membrane
Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER
Protein glycosylation in the rough ER
Oligosaccharides are used as tags to mark the state of protein folding
Misfolded proteins are exported from ER and destroyed
Membrane Transport III Chapter 11. Selectivity of a K + channel.
Intracellular Compartments and Protein Sorting Haixu Tang School of Informatics.
Chapter 12 Intracellular Compartments and Protein Sorting.
Lecture 6 Intracellular Compartments and Protein Sorting.
Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER.
Javad Jamshidi Fasa University of Medical Sciences Proteins Into membranes and Organelles and Vesicular Traffic Moving.
Intracellular Compartments and Protein Sorting Compartmentalization of Cells Pages
Last Class: 1. Posttranscription regulation 2. Translation regulation 3. Cell membrane, phospholipids, cholesterol 4. Membrane protein, mobility, FRAP,
Endomembrane System Yasir Waheed NUST Center of Virology & Immunolgy National University of Sciences &Technology.
Part 1: Intracellular trafficking Week 1: Transport through the nuclear pore complex Week 2: RNA transport, maturation & localization Week 3: ER translocation.
Lecture 6 - Intracellular compartments and transport I.
Review For Final I. Should I take the final? Can’t hurt you Calculate your average and determine what you need to change your grade.
Chapter 26 Protein Sorting. Chapter Objectives Understand the pathways of cotranslational processing of proteins – ER, Golgi, Plasma membrane, Lysosomes.
Protein Sorting & Transport Paths of Protein Trafficking Nuclear Protein Transport Mitochondrial & Chloroplast Transport Experimental Systems Overview.
Chemical reactions in cells need to be isolated. Enzymes work in complexes, spatial distribution in cytosol, nucleus Confinement of reactions in organelle.
Intracellular Transport1 Chapter 15 You should review functions of different organelles We already discussed evolution; review Focus will be on: 1) mechanisms.
Chapter 12 Intracellular Compartments and Protein Sorting 張學偉 助理教授.
MB 207 – Molecular Cell Biology Intracellular compartments and protein sorting.
Protein targeting or Protein sorting Refer Page 1068 to 1074 Principles of Biochemistry by Lehninger & Page 663 Baltimore Mol Cell Biology.
Protein Sorting ISAT 351, Spring 2004 College of Integrated Science and Technology James Madison University.
Chapter 8 Intracellular Compartments and Protein Sorting: Transport Between the Nucleus and the Cytosol Chapter 8 Intracellular Compartments and Protein.
Cellular compartmentalization Pages Q1 Name at least two of the three protein complexes involved in the electron transport chain?
AP Biology Cells gotta work to live! What jobs do cells have to do? make proteins proteins control every cell function make energy for daily.
Protein Localization Chapter Introduction Figure 10.1.
Cytoplasmic Membrane Systems I Lecture 11. The Cellular Compartmentalization Problem of Eukaryotic Cells Cytoplasm: Cytosol plus Organelles Excluding.
Targeting of Proteins to the Organelles. Targeting of Proteins: Nucleus and Mitochondria.
2 Protein Targeting pathways Protein synthesis always begins on free ribosomes In cytoplasm 1) Post -translational: proteins of plastids, mitochondria,
*nucleoid A view of the prokaryotic cell: *plasma membrane *ribosomes *cell wall *capsule *pili Kingdom Monera – archaebacteria and eubacteria.
Chapter 3 Membrane targeting of proteins By D. Thomas Rutkowski & Vishwanath R. Lingappa.
Introduction to Cells How we study cells: *Light microscope microscope *magnification *resolving power *Electron microscope Two basic types: *Transmission.
Unit 7 Endomembranes. SECRETORY PATHWAY: Unit 7 Secretory Pathway Proteins are synthesized on the Rough ER. Move via vesicles to Golgi Move via vesicles.
1 GCCTCAATGGATCCACCACCCTTTTTGGGCA GCCTCAATGGATCCACCACCCTTTTTGGTGCA AGCCTCAATGGATCCACCACCCTTTTTGGTGC AAGCCTCAATGGATCCACCACCCTTTTTGGTG CAAGCCTCAATGGATCCACCACCCTTTTTGGT.
Why organelles? Specialized structures specialized functions cilia or flagella for locomotion Containers partition cell into compartments create different.
Topic 41 4.Structure/Function of the Organelles - Synthesis.
Pg Membrane Protein Movement Single Protein Tethered to cytoskeleton Tethered to other Membrane proteins Pg. 385.
AP Biology Tour of the Cell 1 AP Biology Prokaryote bacteria cells Types of cells Eukaryote animal cells ________________ ________________________.
AP Biology Tour of the Cell 1 AP Biology Prokaryote bacteria cells Types of cells Eukaryote animal cells - no organelles - organelles Eukaryote.
From Cytosol to organelles---post-translational translocation The big picture :
Oocyte injection assay Protein source : In vitro translation.
A Tour of the Cell Chapter 6. Objectives Be familiar with the regions of the cell and the overall role of each region Be familiar with the basic organelles.
Molecular Biology of the Cell Fifth Edition Molecular Biology of the Cell Fifth Edition Chapter 12 Intracellular Compartments and Protein Sorting Chapter.
Nuclear structure Nuclear pore Import/ Export Signals Receptors Ran (Directionality) FG Platform (Gating) Regulation Nuclear Import & Export Overview.
AP Biology Tour of the Cell 1 AP Biology Cells: Introduction to cells- great video 2:55 o2ccTPA
Chapter 4 Plasma membrane, nucleus and ribosomes.
Fundamentals of Cell Biology Chapter 8: Protein Synthesis and Sorting.
Thursday 9/ Mike Mueckler
CELL STRUCTURE Eukaryotic cells contain many organelles: small structures within a cell, sometimes surrounded by a membrane.
Molecular Cell Biology Fifth Edition Chapter 16: Moving Proteins into Membranes and Organelles Copyright © 2004 by W. H. Freeman & Company Harvey Lodish.
Smallest unit of all living things Composed of organelles Each organelle performs specific jobs so the cell can do it’s job.
© 2017 SlidePlayer.com Inc. All rights reserved.