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Tertiary Structure: Supercoiled DNA Figure 29-18Schematic diagram of covalently closed circular duplex DNA that has 26 double helical turns. L = T + W.

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Presentation on theme: "Tertiary Structure: Supercoiled DNA Figure 29-18Schematic diagram of covalently closed circular duplex DNA that has 26 double helical turns. L = T + W."— Presentation transcript:

1 Tertiary Structure: Supercoiled DNA Figure 29-18Schematic diagram of covalently closed circular duplex DNA that has 26 double helical turns. L = T + W L = Linkage Number number of crossings in planar projection T = Topological winding number- (e.g, number of bp/ 10.5 for B-DNA) W = Writhing number- number of turns the duplex axis makes around itself At the left W = 0 so L = T = 26

2 Proc Natl Acad Sci U S A May; 53(5): 1104–1111. J Vinograd, J Lebowitz, R Radloff, R Watson, and P Laipis <95% conversion 5% conversion> of form I to II polyomavirus DNA by DNAse I

3 Proc Natl Acad Sci U S A May; 53(5): 1104–1111. J Vinograd, J Lebowitz, R Radloff, R Watson, and P Laipis

4 Figure 4.18a: Closed covalent circleFigure 4.18b: Singly-nicked circle Figure 4.20a: Linear double-stranded DNA Tropp: Adapted from Bert, J. M., et al. Biochemistry, Fifth Edition. W.H. Freeman and Company, 2002.

5 Figure 4.19: Simulated conformation of supercoiled DNA. Tropp:

6 Bates & Maxwell, 2005

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8 Bates & Maxwell, 2005

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11 Figure 4.20b: Relaxed circle Tropp: Adapted from Bert, J. M., et al. Biochemistry, Fifth Edition. W.H. Freeman and Company, 2002.

12 Figure 4.20d: Unwound circle Tropp: Adapted from Bert, J. M., et al. Biochemistry, Fifth Edition. W.H. Freeman and Company, 2002.

13 Figure 4.20e: Negative supercoil (right-handed) Tropp: Adapted from Bert, J. M., et al. Biochemistry, Fifth Edition. W.H. Freeman and Company, 2002.

14 Figure 4.20c: Linear DNA unwound by two right-handed turns Tropp: Adapted from Bert, J. M., et al. Biochemistry, Fifth Edition. W.H. Freeman and Company, 2002.

15 Figure 4.22b: An underwound covalent circle having only 32 turns of the helix. Figure 4.22a: A nonsupercoiled or relaxed covalent circle having 36 turns of the helix. Figure 4.22c: The molecule in part (b) but with a writhing number of 4 to eliminate the underwinding. Tropp:

16 Figure 4.21b: Negative superhelix Tropp: Adapted from Schvartzman, J. B., and Stasiak, A., EMBO Reports 5 (2004): Figure 4.21c: Positive superhelix

17 Bates & Maxwell, 2005

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23 # Steven A. Wasserman and Nicholas R. Cozzarelli # Science, New Series, Vol. 232, No (May 23, 1986), pp

24 rnatopo.html

25 Figure 4.23: Catalysis of transient breakage of DNA by DNA topoisomerases. Tropp: Adapted from Wang, J. C., Nature Rev. Mol. Cell Biol. 3 (2002):

26 Figure 4.24: Four types of topological coversions catalyzed by topoisomerase I Tropp: Adapted from Kornberg, A., and Baker, T. A. DNA Replication, Second Edition. W.H. Freeman and Company, 1991.

27 Figure 4.25: Escherichia coli topoisomerase I, a type IA topoisomerase. Tropp: Adapted from Champoux, J. J., Annu. Rev. Biochem. 70 (2001):

28 Figure 4.26: Proposed mechanism of relaxation by E. coli topoisomerase I. Tropp: Adapted from Champoux, J. J., Annu. Rev. Biochem. 70 (2001):

29 Figure 4.26f: Proposed mechanism of relaxation by E. coli topoisomerase I. TroppÑ Adapted from Champoux, J. J., Annu. Rev. Biochem. 70 (2001):

30 Figure 4.27: Proposed mechanism for the catalytic cycle of DNA topoisomerase II. TroppÑ Adapted from Larsen, A. K., et al., Pharmacol. Ther. 2 (2003):

31 Bates & Maxwell, 2005

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