Presentation on theme: "The Neurochemistry of GALP: Where Form Meets Function Rachna Goswami Leo Reap."— Presentation transcript:
The Neurochemistry of GALP: Where Form Meets Function Rachna Goswami Leo Reap
What is GALP? Galanin-like peptide (GALP): neuropeptide; only expressed in a small number of neurons within the hypothalamus GALP expression is drastically reduced during fasting, anorexia, and diabetes. GALP acts to alter food intake and metabolic rate, and it also functions to stimulate reproductive physiology and behaviors. The only two factors that regulate GALP are insulin and leptin.
Through our research last summer on pre-pubertal rats we discovered…
Immunocytochemistry Stain brain sections with antibody solutions Assign each antibody a different color through the use of different fluorescent dyes Using fluorescence microscope, we will be able to tell which neural fibers transport GALP and to which areas of the brain they project
Summary Currently, we have: 1)Stereotaxically injected Phaseolus Vulgaris into rat brains 2)Processed brains 3)Successfully done preliminary ICC
Acknowledgments Dr. Fraley HHMI NIH (K01 DK066238-01A1) REACH Hope College