1. Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development Jana Klásková Laboratory of Growth Regulators

2. Cytokinin metabolism

3. Cytokinin degradation

4. IIINhibitors of CYtokinin DEgradation Zatloukal et al., 2008 LGR-2679 LGR-2678

5. Study of inhibitors in biological assays CKX assay

6. Study of inhibitors in biological assays II Receptor assay (CRE1/AHK4) Receptor assay (AHK3)

7. Study of inhibitors in biological assays III ARR5:GUS assay

8. Effect of INCYDE-(F) application by spraying CONTROL 10uM INCYDE-F CONTROL 10uM INCYDE CONTROL 10uM INCYDE-F 10uM INCYDE

9. Effect of INCYDE-F application by spraying Low temperature (15°C) for 4 weeks CONTROL 1 uM 5 uM 10 uM

10. Effect of LGR-2679 application by drain CONTROL 0,1uM X 1x 0,1uM X 3x 0,1uM X 5x 1uM X 1x 1uM X 3x 1uM X 5x CONTROL 0,1uM X 1x 0,1uM X 3x 0,1uM X 5x 1uM X 1x 1uM X 3x 1uM X 5x CONTROL LGR 2679; 0,1uM LGR 2679; 1uM CONTROL 1x3x5x 1x3x5x

11. Effect of LGR-2679 application by drain 0,1uM X 3x 0,1uM X 5x 0,1uM X 8x 0,1uM X 3x 0,1uM X 5x 0,1uM X 8x CONTROL 0,1uM X 3x 0,1uM X 5x 0,1uM X 8x 1uM X 3x 1uM X 5x 1uM X 8x CONTROL LGR 2679; 0,1uM LGR 2679; 1uM 3x5x8x 3x5x8x

12. Mgr. Lukáš Spíchal, Ph.D. Mgr. Ota Blahoušek Mgr. Markéta Gemrotová, Ph.D Mgr. Jaroslav Nisler, Ph.D Thank you for your attention