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Brief Review of DNA Replication:. Nearest Neighbor Analysis (Kornberg 1956): In vitro DNA synthesis Template DNA DNA polymerase dNTPs *dTTP*dCTP*dGTP*dATP.

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Presentation on theme: "Brief Review of DNA Replication:. Nearest Neighbor Analysis (Kornberg 1956): In vitro DNA synthesis Template DNA DNA polymerase dNTPs *dTTP*dCTP*dGTP*dATP."— Presentation transcript:

1 Brief Review of DNA Replication:

2 Nearest Neighbor Analysis (Kornberg 1956): In vitro DNA synthesis Template DNA DNA polymerase dNTPs *dTTP*dCTP*dGTP*dATP Allow DNA replication to take place Denature DNA Treat with micrococcal nuclease- yields mononucleotides with 3’ terminal phosphates

3 A C G T T C ssDNA: * ssDNA treated with micrococcal nuclease: A C G T T C *

4 Exercise: List all possible dinucleotides (analysis of nearest 5’ neighbor) Which must be present in equal frequencies if the DNA strands are: Parallel?Antiparallel?

5 Exercise: List all possible dinucleotides (analysis of nearest 5’ neighbor) ApA ApC ApG ApT GpA GpC GpG GpT CpA CpC CpG CpT TpA TpC TpG TpT Which must be present in equal frequencies if the DNA strands are: Parallel?Antiparallel? ApC = TpGCpA = GpT ApG = TpCCpG = GpC ApT = TpACpT = GpA ApC = GpTCpA = TpG ApG = CpTGpA = TpC

6 Alternative Forms of DNA

7 G + C Content

8 DNA Melting *Temperature at which DNA strands are ½ denatured is the melting temperature or T m *GC content of DNA has a significant effect on T m

9 Comparison of Typical Prokaryotic and Eukaryotic Cells

10 Chromosome Sequence and Diversity Species# of chromosome(s) Form of chromosome(s) Genome size (Mb) PROKAYOTES: Escherichia coli1Circular4.6 Agrobacterium tumifaciens 43 Circular 1 Linear 5.67 EUKARYOTES: Sacharomyces cerevisiae 16Linear12.1 Arabidopsis thaliana 5Linear125 Drosophila melanogaster 4Linear180 Homo sapiens23Linear2,900

11 Gene Density in Different Organisms SpeciesGenome SizeApprox. # of genes Gene density (genes/Mb) PROKAYOTES: Escherichia coli4.64, Agrobacterium tumifaciens 5.75, EUKARYOTES: Sacharomyces cerevisiae 125, Arabidopsis thaliana 12525, Drosophila melanogaster 18013,70080 Homo sapiens2,90027,0009.3


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