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C-myc paper reviewer’s suggestions Use additional SERCA inhibitor to show affects on c-myc, egr-1 and MAPKs Look at c-myc and egr-1 at the protein level.

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Presentation on theme: "C-myc paper reviewer’s suggestions Use additional SERCA inhibitor to show affects on c-myc, egr-1 and MAPKs Look at c-myc and egr-1 at the protein level."— Presentation transcript:

1 C-myc paper reviewer’s suggestions Use additional SERCA inhibitor to show affects on c-myc, egr-1 and MAPKs Look at c-myc and egr-1 at the protein level when induced with Tg. Use U73343 which is the less active analog of the PLC inhibitor U73122 to control for non-specific effects

2 What now Treat Perk+/+ and Perk-/- MEFs with cyclopiazonic acid (CPA) –Look at eIF2a phosphorylation –Assess c-myc and egr-a at RNA and protein level Treat Perk+/+ and Perk-/- MEF with Tg –Look at c-myc and egr-1 at protein level

3 WT KO WT KO WT KO WT UT CPA hr CPA 2hr Tg WT KO WT KO WT KO WT UT CPA hr CPA 2hr Tg eIF2a eIF2a[P] Perk+/+ and Perk-/- MEFs treated with 10  M CPA

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