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Scott Zawko 2012.11.19 Data Blitz. Bioresorption and Efficacy Study.

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Presentation on theme: "Scott Zawko 2012.11.19 Data Blitz. Bioresorption and Efficacy Study."— Presentation transcript:

1 Scott Zawko Data Blitz

2 Bioresorption and Efficacy Study

3 Sodium displacement of calcium Na + Ca 2+ Na + Ca 2+

4 Calcium chelation Citrate EDTA Na + Ca 2+ Na + Ca 2+ Chelator

5 Surgical procedure

6 Rat cecal abrasion studies DateFilmsChelatorOutcome July 2012 Templated Non-templated EDTA or Citrate 0.2 mL 100 mM 34 of 35 films did not degrade

7 Chelator treatment promotes degradation in rat carcass

8 Rat cecal abrasion studies DateFilmsChelatorOutcome July 2012 Templated Non-templated EDTA or Citrate 0.2 mL 100 mM 34 of 35 films did not degrade Oct 2012 Templated Non-templated Citrate 0.5 mL 300 or 400 mM 5 mg/mL HA 4 of 38 films did not degrade

9 Group Film Found? Yes No Totals Film8210 Film, 300 mM citrate18 9 Film, 400 mM citrate1910 Film, templated8210 Film, templated, 300 mM citrate18 9 Film, templated, 400 mM citrate1910 Film FoundChelatorNo ChelatorTotals Yes04 (11%)16 (80%)20 No34 (89%)04 (20%)38 Totals p <

10 Grade scale for adhesion severity

11 Group Adhesion Grade p or 3 Control SepraFilm Film, no chelator Film, 300 mM citrate Film, 400 mM citrate Film, templated, no chelator Film, templated, 300 mM citrate Film, templated, 400 mM citrate Incidence of adhesions

12 Swelling measurements

13 Swelling formulas

14 Prelim swelling measurements Trial #BlottingWet Weight (mg) 1none204 2none178 3none244 4none197 5blot on glove125 6blot on glove128 7blot on glove134 8more blotting106 9more blotting108 10more blotting101

15 Prelim swelling measurements Trial #Weight (mg) Average105 Stdev2.9

16 Wet Wt (mg) Thickness (um) Film #123AvgStdev Dry Wt (mg) SR% WCMinMax Average4.582 Stdev Swelling data – HA/Alg Film

17 SR weakly correlated with thickness Pearson’s Correlation Coefficient, R = 0.58

18 HA increases film swelling

19

20 Film thickness measurements

21 21 Film thickness Thickness (um) in Thickness (um)

22 Leveling is very important Top Surface Bottom Surface Shelf

23 Films Thickness (μm) Thickness (um) Re-leveled 2.7 in Not-leveled

24 Leveling is difficult 2.7 in 10 µm θ

25 Doctor blade for film casting

26

27 Solution castingDoctor blading Cast in a moldSpread on a flat surface without sides Use a solution with low viscosityUse a solution with high viscosity Variables: concentration, volumeVariables: concentration, gap height Requires high precision machined molds (Cost: $100's) Requires a doctor blade (Cost: $100's) Leveling is absolutely necessaryLeveling is not necessary Film thickness is non-uniformFilm thickness is uniform Contrast two techniques

28 Doctor Blade Films Gap height: 750 – 1000 μm750 μm 1500 μm 1000 μm 1.7 in 3.5 in

29 Doctor Blade Films Gap height (µm) Films Thickness (μm)

30 Film # Thickness (μm) MinMaxRange Doctor Blade Films

31


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