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Gram negative rod of non Enterobacteriacheae Suryanie Sarudji, MKes., DVM.

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Presentation on theme: "Gram negative rod of non Enterobacteriacheae Suryanie Sarudji, MKes., DVM."— Presentation transcript:

1 Gram negative rod of non Enterobacteriacheae Suryanie Sarudji, MKes., DVM

2 Gram negative rod of non Enteric Pseudomonas sp Pasteurella sp Vibrio sp dan Campylobacter sp Brucella sp Haemophylus sp Bordetella sp Maleomyces sp

3 Genus Pseudomonas

4 Clasification of Pseudomanas Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Pseudomanadales Family: Pseudomonadaceae Genus: Pseudomonas Malleomyces (Burkholderia) Spesies: Ps. Aeruginosa, Malleomyces (Burkholderia) mallei M. (B) pseudomallei.

5 Obligate aerobe, oxidaze +, Catalase +, they are motile with 1 or more than 1 polar flagella, except Burkholderia (Malleomyces) mallei are non motile Growth perfectly on MCA, yield pigment which melt in water and have opportunistic properties Ps. Fluorescens & Ps. putida infect fish of fresh water

6 They are environmental bacteria in water, land and plants. Ps.aeruginosa are found in skin, m. mucosa and feces Rod shape of = 0,5 X 1,0 – 3,0 micron, not form spore and not have capsule Pigment : pyocyanine (green-blue) and fluorescence growth in simple medium, aerobe

7 CharacterPs.aeruginosaM.malleiM. pseodomallei Colony BA Pigment MCA Growth at 42oC Oxidase Motil Oxides: - Glucose - Lactose - Sucrose large, evently Hemolytic + - white, smooth smooth-muccoid-rough Hemolysic - +

8 Malleomyces sp M. mallei  Ingus jahat Disease = Malleus = Glanders M. pseudomallei  Glanders like disease = Ingus jinak Disease = Melioidosis M. mallei Rod shape, In old culture are pleomorphic, bipolar, non motile, Gram -. Grow in environment of aerobe and facultative anaerobe. Colony is brown - yellowish

9 Non fermented, indol, nitrate, gelatin, MR-Vp negative Catalase and H2S positive M. pseudomallei Produce acid from glucose, maltose, manitol, lactose and sucrose Indol and H2S negative, to melt gelatin

10 Infection cause by Ps. Aeruginosa Cattle : Mastitis, metritis, pneumonia,dermatitis, enteritis Sheep : Mastitis, pneumonia, otitis media Pig : Otitis, infection at upper of respiratoriuos tract Horse : Infection at Genital tract, pneumonia, ceratitis Dog & Cat : external otitis, Cystitis, Pneumonia, ulcerative ceratitis

11 Genus Pasteurella

12 Clasification of Pasteurella: Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Pasteurellales Family: Pasteurellaceae Genus: Pasteurella Spesies: P. multocida, haemolytica, dll

13 Small and short rod/coccobacillus/coccoid bipolar. Non motile, Gr -, Oxidase +, catalase +, Facul tative anaerob, grow on medium which are added by blood or serum. P. Haemolitica tolerant to bile salt also able to grow on MCA P. Multocida type A : poultry B : Cattle, Buffalo and Pig P. haemolytica : Just to Cattle

14 On TSA : There are 2 colony : fluorescence and blue Fluorescent Colony: large, white, muccoid, pathogen Blue Colony: small as like as drip of dew 2 type of P. multocida : A. Arabinosa and ducitol fermented but non xylosa fermented B. Xylosa fermented but non arabinosa and ducitol fermented

15 CharacterP. multocidaP. haemolitica BA MCA Indol Catalase Urease Ornithindecarboxylase Acid from : - Lactose - Sucrose - Maltose - D-xylose - L-arabinose - D-trehalose - Capsule from tissue Non hemolytic v + Hemolytic v -

16 Vibrio and Campylobacter

17 Klasifikasi Vibrio and Campylobacter Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Vibrionales Family: Vibrionaceae Genus: Vibrio, Campylobacter Spesies: V. cholera, C. fetus dll

18 Morphology : crooked rod/comma Motile with polar flagella, catalase +, hemolize blood, not grow on MCA, grow in NB with NaCl 6%, Oxidase +, require sucrose, mannitol variable. Live in an aquatic, opportunistic in fish mammalia, human

19 Organism which are classified as Vibrio but have microaefilic property are grouped as Campylobacter Diffrences Vibrio and Campylobacter is Campylobacter is indol – Vibrio grow perfect aerobically on TCBS medium

20 Many spesies of Vibrio and the disease caused by Vibrio : V. comma = V. cholera  Cholera to human V. metchnikovii  gastroenteritis to chicken Diffrences between V. comma/cholera and V. metchnikovii is V. comma coagulate milk whereas V. metchnikovii isn’t. Both of them yield indol from tryptophan and melt gelatin.

21 Campylobacter sp

22 Cylinder shape, crooked rod Gram - Motile with polar flagella Live in the condition of microaerofilic, grow at high pressure of CO2 (min 10%) and low pressure of O2. Grow on MCA, non fermentative, oxidase +, catalase variable. Find in digestives tract, genital tract of animals Infection to intestine cause : diarrhea; genital : abortion, infertility

23 Pathogen species in Veterinary : - Campylobacter fetus subspeciesveneralis - Campylobacter fetus subspeciesfetus - Campylobacter jejuni

24 Campylo bacter spp C. Fetus sbsps veneralis Bovin reproductive tract Venereal disease In catle, early embryonic death Tempory infertility C. Fetus sbsps veneralis Instestinal tract of sheep Goats & catle abortion C. jejuni Instestinal tract of birds & mammalia Abortion in sheep Infertility in dogs Avian hepatitis Enterocolitis in human

25 Species bacteria Catalase Growth at Growth in 1 % glycin Growth in 3 % NaCl H2S 25oC42oC C.f.vene- ralis C.f.fetus C. Jejuni

26 Brucella sp

27 Morfologi kuman Brucella sp

28 Clasificaion of Brucella Kingdom: Bacteria Phylum: Proteobacteria Class: Alpha Proteobacteria Order: Rhizobiales Family: Brucelliaceae Genus: Brucella Spesies: B. abortus, canis, melitensis, suis, ovis

29 Morphology : rod coccoid/coccobacilli Gram negative, non motile Stain by 0.5 % acidic so considered as acid fast or by Modified Ziel Nelson (MZN) is positive In MZN appearance as cluster coccobacillus and red color For taxonomy according to DNA study all of species of Brubella are classified as Brucella millitensis

30 Live aerobically, catalase +, oxidase +, urease + just only ovis is urease - Br. Ovis and many biotype of Br. abortus utilize 5 – 10 % of CO2 for primer isolation The growth increase when CO2 is high. For the growth the Medium must be rich with blood or serum

31 Species Necessity of CO2 H2S Urease Growth on media containing ThioninBasic fuchsine Abortus Millitensis Suis Ovis Canis v--+-v--+- v-v--v-v-- +v+-++v+-+ v++++v++++ v+v--v+v--

32 Bordetella sp

33 Clasification of Bordetella Kingdom: Bacteria Phylum: Proteobacteria Class: Beta Proteobacteria Order: Burkholderiales Family: Alcaligenaceae Genus : Bordetella Spesies: B. pertusis, parapertusis, bronchiseptica

34 3. Species : Bordetella pertusis Bordetella para pertusis Bordetella bronchiseptica The morphology of bacteria are small, Coccobacilli, solitary, in pair, and be in small cluster, Gr- Primarily isolation : the form of cell are uniform Subculture : to be very pleomorphic, filament, thick Stained by toluidin blue appear bipolar

35 B. pertusis, B. parapertusis non motile; B. bronchiseptica motile with lateral flagella, have capsule, aerobic bacteria, the pathway of CH metabolism is respiration, never do fermentation. Isolation primarily require adding of charcoal, resin (as an ion exchange), or % blood to ovoid influence inhibiting the growth of substance as like as patty acid, colloidal sulfur, sulfide, peroxide

36 On modifikation of Bordet-Gengou Medium (potato-glycerol-blood agar) B. pertusis colony smooth, convex, transparent, shine brightly All species are haemolysis

37 Identification B. pertusis B. parapertusis B. Bronchiseptica Motility--+ Reduced Nitrate Citrate-++ Urease-++ G on peptone agar-++ chocolate in peptone A-+- G on Bordet Gengou Agar -3-4 hr 1-2 hr1-2 hr Litmus milk-(12-14 hr)+(1-4 hr)+(1-4 hr) G + C616166

38 Haemophylus sp

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40 Clasification of Haemophillus Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Pasteurellales Family: Pasteurellaceae Genus: Haemophillus Spesies: H. gallinarum, paragallinarum, influenza dll

41 Morphology of bacteria are small rod, Gr- Occasionally appear coccobacilli or short filament, they are motile, facultative anaerobe, catalase and oxidase are variable, not grow on MCA The bacteria require X (haem) and V (Nicotinamide adenine dinucleatid = NAD) factor for growing Optimal concentrate of CO2 is 5-10 % in Chocolate agar.

42 Morphology of colony are small like drop of dew, transparant, after 48 hours inoculation. Comencial on membrane mucosa of animal’s respiratory tract Sucrose and mannitol are fermented

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