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Conclusion Immune cells in the periphery are capable of producing IL-1F9Immune cells in the periphery are capable of producing IL-1F9 The cellular sub-type.

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Presentation on theme: "Conclusion Immune cells in the periphery are capable of producing IL-1F9Immune cells in the periphery are capable of producing IL-1F9 The cellular sub-type."— Presentation transcript:

1 Conclusion Immune cells in the periphery are capable of producing IL-1F9Immune cells in the periphery are capable of producing IL-1F9 The cellular sub-type that is primarily producing this cytokine was identified through magnetic separation of monocytes from the PBMCThe cellular sub-type that is primarily producing this cytokine was identified through magnetic separation of monocytes from the PBMC This result has implications for both the innate and adaptive immune response, not only in patients with RRP, but for immunology in generalThis result has implications for both the innate and adaptive immune response, not only in patients with RRP, but for immunology in general Selected References Armstrong, L. R., C. S. Derkay and W. C. Reeves. “ Initial Results from the National Registry for Juvenile-onset Recurrent Respiratory Papillomatosis. RRP Task Force. Arch Otolaryngol Head Neck Surg. Jul 1999: 125(7):743-8Armstrong, L. R., C. S. Derkay and W. C. Reeves. “ Initial Results from the National Registry for Juvenile-onset Recurrent Respiratory Papillomatosis. RRP Task Force. Arch Otolaryngol Head Neck Surg. Jul 1999: 125(7):743-8 Berglof, E., Ralph Andre, Blair R. Renshaw, Stuart M. Allan, Catherine B. Lawrence, Nancy J. Rothwell and Emmanuel Pinteaux. “IL-1Rrp2 Expression and IL-1F9 (IL-1H1) Actions in Brain Cells” J Neuroimmunol (2003): 139:36Berglof, E., Ralph Andre, Blair R. Renshaw, Stuart M. Allan, Catherine B. Lawrence, Nancy J. Rothwell and Emmanuel Pinteaux. “IL-1Rrp2 Expression and IL-1F9 (IL-1H1) Actions in Brain Cells” J Neuroimmunol (2003): 139:36 Bishai D, H. Kashima and K. Shah. “The Cost of Juvenile-onset Recurrent Respiratory Papillomatosis.” Arch Otolaryngol Head Neck Surg. Aug 2000; 126(8);935-9.Bishai D, H. Kashima and K. Shah. “The Cost of Juvenile-onset Recurrent Respiratory Papillomatosis.” Arch Otolaryngol Head Neck Surg. Aug 2000; 126(8); Bonagura, Vincent R., Frederick P. Siegal, Allan L. 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"Chracterization of Global Transcription Profile of Normal and HPV-immortalized Keratinocytes and their Response to TNF Treatment." BMC Medical Genomics. 1 (2008): 29. Termini, Lara, Enrique Boccardo, Gustavo H. Esteves, Roberto Hirata, Waleska K. Martins, Anna Estela L. Colo, E Jordao Neves, Luisa L. Villa, and Luiz Reis. "Chracterization of Global Transcription Profile of Normal and HPV-immortalized Keratinocytes and their Response to TNF Treatment." BMC Medical Genomics. 1 (2008): 29. Towne, J. E., K. E. Garka, B. R. Renshaw, G. D. Virca, and J. E. Sims. “Interleukin (IL)-1F6, IL-1F8, and IL-1F9 Signal through IL-1Rrp2 and IL-1RAcP to Activate the Pathway Leading to NF-_B and MAPKs” J Biol Chem (2004):279: Towne, J. E., K. E. Garka, B. R. Renshaw, G. D. Virca, and J. E. Sims. “Interleukin (IL)-1F6, IL-1F8, and IL-1F9 Signal through IL-1Rrp2 and IL-1RAcP to Activate the Pathway Leading to NF-_B and MAPKs” J Biol Chem (2004):279: Yang R, Murillo FM, Cui H, et al. “Papillomavirus-like Particles Stimulate Murine Bone Marrow-derived Dendritic Cells to Produce Alpha Interferon and Th1 Immune Responses via MyD88.” J Virol. 2004;78: Yang R, Murillo FM, Cui H, et al. “Papillomavirus-like Particles Stimulate Murine Bone Marrow-derived Dendritic Cells to Produce Alpha Interferon and Th1 Immune Responses via MyD88.” J Virol. 2004;78: Discussion CD154A, IFN-γ, and IL-10 genes all showed significant stimulation in most of the tested conditions; however, the IL1F9 gene only showed a response to PWM and PHA.CD154A, IFN-γ, and IL-10 genes all showed significant stimulation in most of the tested conditions; however, the IL1F9 gene only showed a response to PWM and PHA. There are 10 cycle difference for PWM and the 5 cycle difference for PHA represent roughly a 1000x fold increase and a 32x fold increase for these two mitogens.There are 10 cycle difference for PWM and the 5 cycle difference for PHA represent roughly a 1000x fold increase and a 32x fold increase for these two mitogens. IL1F9 gene, which shows significantly elevated expression in papilloma tissue from patients with severe RRP, is mainly produced by the monocytes in PBMC.IL1F9 gene, which shows significantly elevated expression in papilloma tissue from patients with severe RRP, is mainly produced by the monocytes in PBMC. Future Research Further studies will focus on the primary role and function of IL1F9 on innate and adaptive immune response.Further studies will focus on the primary role and function of IL1F9 on innate and adaptive immune response. Future studies will involve papilloma tissue or patient sample to directly examine the new findings from this study in actual patients.Future studies will involve papilloma tissue or patient sample to directly examine the new findings from this study in actual patients.


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