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MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski Department of Molecular and Biometric Techniques, Museum and Institute.

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Presentation on theme: "MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski Department of Molecular and Biometric Techniques, Museum and Institute."— Presentation transcript:

1 MITOGENOME FRAGMENTATION IN ANIMALS – POSSIBLE MOLECULAR MECHANISMS Tadeusz Malewski Department of Molecular and Biometric Techniques, Museum and Institute of Zoology, Polish Academy of Science, Warsaw, Poland

2 Biochimica et Biophysica Acta 1819 (2012): Typical organization of mtDNA in animals

3 Multipartite mitochondrial DNA Wei D-D, Shao R, Yuan M-L, Dou W, Barker SC, et al. (2012) The Multipartite Mitochondrial Genome of Liposcelis bostrychophila: Insights into the Evolution of Mitochondrial Genomes in Bilateral Animals. PLoS ONE 7(3): e doi: /journal.pone

4 Gibson T, Blok VC, Phillips MS, Hong G, Kumarasinghe D, Riley IT, Dowton M (2007) The Mitochondrial Subgenomes of the Nematode Globodera pallida are Mosaics: Evidence of Recombination in an Animal Mitochondrial Genome J Mol Evol 64:463–471 Multipartite mitochondrial DNA

5 Smith DR, Kayal E, Yanagihara AA, Collins AG, Pirro S, Keeling PJ (2011) First Complete Mitochondrial Genome Sequence from a Box Jellyfish Reveals a Highly Fragmented Linear Architecture and Insights into Telomere Evolution Genome Biol. Evol. 4(1):52–58. Multipartite mitochondrial DNA

6 Shao R, Zhu X-Q, Barker SC, Herd K (2012) Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans Genome Biol. Evol. 4(11):1088–1101 Multipartite mitochondrial DNA

7 Shao R, Zhu X-Q, Barker SC, Herd K (2012) Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans Genome Biol. Evol. 4(11):1088–1101

8 Multipartite mitochondrial DNA number of minicycles Hexapoda Liposcelis bostrychophila – 2 Damalinia sika – 3 Pediculus humanus – 20 NematodaGlobodera pallida – 7 Globodera rostrochiensis – 7 RotiferaBrachionus plicatilis – 2 MedusozoaHydra magnipapillata– 2 MesosoaDicyema misakiense – 6 DiplonemidsDiplonema papillatum > 100

9 Possible molecular mechanisms responsible for formation and maintaining of multipartite mtDNA - replication - recombination

10 Kasiviswanathan R, Collins TRL, Copeland WC (2012) The interface of transcription and DNA replication in the mitochondria Biochimica et Biophysica Acta 1819: 970–978

11 Klingbeil MM, Shapiro TA (2009) Unraveling the Secrets of Regulating Mitochondrial DNA Replication Molecular Cell 35: Silencing of TbPIF2 expression resulted in rapid loss of maxicircles with little effect on minicircles Abundance of TbPIF2 is controlled through proteolytic degradation by TbHslVU

12 RNA (?) helicase Suv3 M. Minczuk, J. Piwowarski, M.A. Papworth, K. Awiszus, S. Schalinski, A. Dziembowski, A. Dmochowska, E. Bartnik, K. Tokatlidis, P.P. Stepien, P. Borowski (2002) Localisation of the human hSuv3p helicase in the mitochondrial matrix and itspreferential unwinding of dsDNA. Nucleic Acids Res. 30: 5074–5086. Comparison of efficacy of RNA and DNA unwinding reaction mediated by hSuv3p as a function of decreasing enzyme concentration.

13 Szczesny RJ, Borowski LS, Malecki M, Wojcik MA, Stepien PP, Golik P (2012) RNA Degradation in Yeast and Human Mitochondria Biochimica et Biophysica Acta 1819: 1027–1034 A model of the functional interplay between the activities of the yeast mitochondrial degradosome.

14 Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e doi: /journal.pone

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16 Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e doi: /journal.pone

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18 Alverson AJ, Zhuo S, Rice DW, Sloan DB, et al. (2011) The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats. PLoS ONE 6(1): e doi: /journal.pone

19 Shao R, Zhu X-Q, Barker SC, Herd K (2012) Evolution of Extensively Fragmented Mitochondrial Genomes in the Lice of Humans Genome Biol. Evol. 4(11):1088–1101 Multipartite mitochondrial DNA

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21 GGTTTTTCG T-A Scmt I GGTTTTTCG A-T Scmt III TATTCTTTCC T-A A-T T-A Scmt VII TTCTCGGTC T-A A-T T-A Scmt VI TTAATTAT T-A C-G T-A A-T Scmt VI Stem-loop structures in multipartite mtDNA of Globodera G. pallida G. rostrochiensis

22 ABCACB Scmt II Scmt I Scmt III Scmt IV Scmt V Scmt VI Variations in multipartite mtDNA in populations of Globodera pallida

23 mtDNAPopulations ABC Cysts I II III IV V VI VII

24 Thank you for attention


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