1. Plant Cell Respiration of Germinating Seeds Mitochondria
organelles where cellular respiration occurs
Respiration of Germinating Seeds
r1.04

2. Introduction The Snapshot button is used to capture the screen.
Respiration of Germinating Seeds
Introduction
Each page of this lab that contains the symbol
should be inserted into your journal. After completing a lab page with the snapshot symbol, tap (in the upper right hand corner) to insert the page into your journal.
Journals and Snapshots
The Snapshot button is used to capture the screen.
The Journal is where snapshots are stored and viewed.
The Share button is used to export or print your journal to turn in your work.
Note: You may want to take a
snapshot of the first page of
this lab as a cover page for
your journal.

3. Respiration of Germinating Seeds
Lab Challenge
When plant seeds carry out "cellular respiration" they produce carbon dioxide.
Does temperature affect the rate at which seeds produce carbon dioxide (CO2)?
Do dormant seeds or geminating seeds produce more CO2?

4. Respiration of Germinating Seeds
Background
All living organisms need energy to survive, grow, and reproduce.
Organisms get their energy in the form of ATP (Adenosine Triphosphate), a small molecule packed with energy.
Organisms convert sugars and other macromolecules into ATP molecules through a process called cellular respiration.

5. Self-Check What is the main function of ATP in living organisms?
Respiration of Germinating Seeds
Self-Check
What is the main function of ATP in living organisms?
respiration
triphosphate
motion
energy
This image is a reminder to
tap to take a snapshot
of the page after you have
entered your response.
L6 – Text Box
[The correct choice is …]

6. ...Background The chemical reaction for cellular respiration is:
Respiration of Germinating Seeds
...Background
The chemical reaction for cellular respiration is:
C6H12O6 + 6O > 6CO2 + 6H2O + Energy (ATP molecules)
CO2 is a measurable end product of the reaction.
The amount of CO2 created indicates the rate of
cellular respiration occurring inside an organism.
The process of cellular respiration occurs in the
mitochondria of all eukaryotic cells.

7. Respiration of Germinating Seeds
Self-Check
Why does this lab involve measuring the amount CO2 produced by seeds?
indicates rate of velocity
indicates rate of mortgage interest
indicates rate of cellular respiration
indicates heart-rate
L6 – Text Box
[The correct choice is …]

8. Pre Lab Set Up 24 hours prior to the lab:
Respiration of Germinating Seeds
Pre Lab Set Up
(Your teacher may have already completed these steps for you)
24 hours prior to the lab:
Place 20 dry seeds in a beaker and fill the beaker with tap water.
Put the beaker in a dark location for 24 hours. The seeds will begin to germinate.
30 minutes prior to the lab:
Place half of the germinating seeds into an ice water bath.
Allow them to cool before starting the experiment.

9. Safety Use all standard laboratory safety procedures.
Respiration of Germinating Seeds
Safety
Use all standard laboratory safety procedures.
DO NOT allow the Carbon Dioxide Gas Sensor to get wet.

10. Materials and Equipment
Respiration of Germinating Seeds
Materials and Equipment
Collect all of these materials before beginning the lab.
PASPORT Carbon Dioxide Gas Sensor
PASPORT Extension cable
Sampling bottle (included with sensor)
1 Beaker (1000 mL)
10 dry seeds
Ice (cubes or crushed)
Paper towels
Tap water
10 germinating seeds at room temperature
10 germinating seeds in ice water
CO2 Gas Sensor
PASPORT Extension Cable

11. Sequencing Challenge Respiration of Germinating Seeds
The steps to the left are part of the procedure for this lab activity. They are not in the right order. Determine the correct sequence of the steps, then take a snapshot of this page.
A. Analyze the data.
B. Measure the CO2 produced by dormant seeds.
C. Measure the CO2 produced by germinating seeds.
D. Measure the CO2 produced by cold seeds.
L6 – Text Box
[The correct sequence is …]

12. Respiration of Germinating Seeds
Setup: Dormant Seeds
Q1: Why is it important to firmly insert the sensor into the bottle and create a seal?
Connect a PASPORT extension cable to the SPARK Science Learning System. Plug the CO2 Gas Sensor in the cable and then press the green button on the sensor to calibrate it.
Place 10 dormant (dry) seeds into the sampling bottle.
Put the end of the CO2 Gas Sensor into the sampling bottle. Firmly plug the end of the sampling bottle with the rubber stopper. Do not let the bottle tip over!
L6 – Text Box
[It is important to create a seal…]

13. Respiration of Germinating Seeds
Q2: What will happen to the carbon dioxide level inside the bottle? Draw your prediction in the graph.*
*To Draw a Prediction:
1. Tap to open the tool palette.
2. Tap then use your finger to draw your prediction.
3. Tap when finished.
4. If you make a mistake, tap to clear your prediction.

14. Collect Data: Dormant Seeds
Respiration of Germinating Seeds
Collect Data: Dormant Seeds
Q3: Why do you need to rinse out the sample bottle?
Note: Avoid jarring the sample bottle during data collection, as this can cause erratic results.
Tap to begin collecting data.
Record data for 10 minutes, then tap to end data collection.
Remove the sensor and the dormant (dry) seeds from the sample bottle.
Rinse the sample bottle out with tap water.
Continue to the next page.
L6 – Text Box
[You need to rinse out the sample bottle…]

15. Respiration of Germinating Seeds
Q4: What happened to the amount of CO2 produced by the dry seeds over time?
L6 – Text Box
[The amount of CO2 produced by the dry seeds…]

16. Respiration of Germinating Seeds
Q5: What will happen to the CO2 level when germinating seeds are placed in the bottle?
Draw your prediction in the graph.*
*To Draw a Prediction:
1. Tap to open the tool palette.
2. Tap then use your finger to draw your prediction.
3. Tap when finished.
4. If you make a mistake, tap to clear your prediction.

17. Collect Data: Room-Temp Seeds
Respiration of Germinating Seeds
Collect Data: Room-Temp Seeds
Place ten of the room-temperature germinating seeds into the sample bottle.
Re-insert the sensor into the bottle.
Tap to begin collecting data.
Record data for 10 minutes, then tap to end data collection.
Remove the sensor and the dormant (dry) seeds from the sample bottle.
Rinse the sample bottle out with tap water.

18. Respiration of Germinating Seeds
Q6: What happened to the amount of CO2 produced by the germinating seeds over time?
L6 – Text Box
[The amount of CO2 produced by the germinating seeds…]

19. Respiration of Germinating Seeds
Q7: How did your predicted graph line compare with the actual line?
L6 – Text Box
[My predicted line and actual line…]

20. Respiration of Germinating Seeds
Q8: What will happen to the CO2 level when germinating seeds are placed in the bottle?
Draw your prediction in the graph.*
*To Draw a Prediction:
1. Tap to open the tool palette.
2. Tap then use your finger to draw your prediction.
3. Tap when finished.
4. If you make a mistake, tap to clear your prediction.

21. Collect Data: Chilled Seeds
Respiration of Germinating Seeds
Collect Data: Chilled Seeds
Fill the 1000 mL beaker with ice and water.
Place ten of the chilled germinating seeds into the sample bottle. Place the bottle into the icy beaker so the seeds stay cold!
3. Re-insert the sensor into the bottle.
Tap to begin collecting data.
Record data for 10 minutes, then tap to end data collection.
Remove the sensor and the chilled seeds from the sample bottle.
Rinse the sample bottle out with tap water.

22. Respiration of Germinating Seeds
Q9: What happened to the amount of CO2 produced by the chilled germinating seeds over time?
L6 – Text Box
[The amount of CO2 produced by the chilled seeds…]

23. Respiration of Germinating Seeds
Q10: How did your predicted graph line compare with the actual line?
L6 – Text Box
[My predicted line and actual line…]

24. Data Analysis Respiration of Germinating Seeds
Determine the change in CO2 concentration for all runs, and record the information in the data table on the next page.
* To Find the Difference Between Two Data Points:
Tap to open the tools palette.
Tap and then tap two points on the data run.
Adjust using both buttons and then tap
Tap to display the differences.

25. Data Analysis Respiration of Germinating Seeds
Record the initial, final, and change in CO2 concentration for all runs, in the data table to the left.
*To Enter Data into a Table:
1. Tap to open the tool palette.
2. Tap then tap a cell in the data table to highlight it in yellow.
3. Tap to open the Keyboard screen.
L1245 – Table
Col 1: [Condition: Dry seeds, Germinating. room temp, Germinating. Cold]
Col 2: [Initial CO2 (ppm)]
Col 3: [Final CO2 (ppm)
Col 4: [Change in CO2 (ppm)]

26. Respiration of Germinating Seeds
Analysis
How does the rate of CO2 production for dormant seeds compare to the rate of CO2 production for room-temperature germinating seeds? What does this information indicate?
L45 – Text Box
[The rate of CO2 production for germinating versus dry seeds…]
[This indicates that…]

27. Respiration of Germinating Seeds
Analysis
How does the rate of CO2 production for room-temperature geminating seeds compare to the rate of CO2 production for chilled germinating seeds? What does this information indicate?
L456 – Text Box
[The rate of CO2 production between room temperature germinating seeds versus chilled germinating seeds…]
[This indicates…]

28. Respiration of Germinating Seeds
Analysis
How did your predictions compare to the actual data collected? What did you learn?
L4 – Text Box
[My predictions…]
[I learned that…]

29. Respiration of Germinating Seeds
Synthesis
If cellular respiration occurs during seed germination, what would happen to CO2 levels if you added water to a sample bottle filled with dry peas?
L45 – Text Box
[I added water to a sample bottle that contains dry seeds…]

30. Respiration of Germinating Seeds
Synthesis
What is the chemical equation for cellular respiration? Where does this process occur in the cell?
L4 – Text Box
[The equation for cellular respiration is…]
[It occurs…]

31. Respiration of Germinating Seeds
Synthesis
What gaseous molecule is released during cellular respiration?
L45 – Text Box
[The gaseous molecule released during cellular respiration is…]

32. Respiration of Germinating Seeds
Synthesis
Explain why plants need to perform cellular respiration even though they are photosynthetic.
L456 – Text Box
[Even though plants are photosynthetic, they must perform cellular respiration because…]

33. Respiration of Germinating Seeds
Synthesis
What environmental factors can affect the rate of cellular respiration in an organism?
L45 – Text Box
[Environmental factors that can affect cellular respiration include…]

34. Respiration of Germinating Seeds
Multiple Choice
Cellular respiration takes place in what part of the cell?
chloroplasts
nuclei
lysosomes
mitochondria
L6 – Text Box
[The correct choice is…]

35. Respiration of Germinating Seeds
Multiple Choice
In cells, the energy stored in glucose is used to make what energy rich compound?
water
ATP
ADP
DNA
L6 – Text Box
[The correct choice is…]

36. Multiple Choice 5-10 minutes 180-240 seconds 1200-1800 seconds
Respiration of Germinating Seeds
5-10 minutes
seconds
seconds
25-30 minutes
Multiple Choice
According to the graph, the rate of CO2 production was highest at what time?
L6 – Text Box
[The correct choice is…]

37. Respiration of Germinating Seeds
Congratulations!
You have completed the lab.
Please remember to follow your teacher's instructions for cleaning-up and submitting your lab.
Color scheme (RGB values)
biology 121, 173, 54
chemistry 0 176, 216
physics 0, 102, 204
earth 204, 102, 0
middle 153, 102, 153
elementary 255, 51, 0

38. References Respiration of Germinating Seeds
Images are taken from PASCO documentation, public domain clip art, or Wikimedia Foundation Commons.