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Mitochondria organelles where cellular respiration occurs Plant Cell Respiration of Germinating Seeds 012-10975 r1.04.

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Presentation on theme: "Mitochondria organelles where cellular respiration occurs Plant Cell Respiration of Germinating Seeds 012-10975 r1.04."— Presentation transcript:

1 Mitochondria organelles where cellular respiration occurs Plant Cell Respiration of Germinating Seeds r1.04

2 The Snapshot button is used to capture the screen. The Journal is where snapshots are stored and viewed. The Share button is used to export or print your journal to turn in your work. Introduction Journals and Snapshots Respiration of Germinating Seeds Each page of this lab that contains the symbol should be inserted into your journal. After completing a lab page with the snapshot symbol, tap (in the upper right hand corner) to insert the page into your journal. Note: You may want to take a snapshot of the first page of this lab as a cover page for your journal.

3 Lab Challenge When plant seeds carry out "cellular respiration" they produce carbon dioxide. Does temperature affect the rate at which seeds produce carbon dioxide (CO 2 )? Do dormant seeds or geminating seeds produce more CO 2 ? Respiration of Germinating Seeds

4 Background All living organisms need energy to survive, grow, and reproduce. Organisms get their energy in the form of ATP (Adenosine Triphosphate), a small molecule packed with energy. Organisms convert sugars and other macromolecules into ATP molecules through a process called cellular respiration. Respiration of Germinating Seeds

5 Self-Check 1.What is the main function of ATP in living organisms? a)respiration b)triphosphate c)motion d)energy Respiration of Germinating Seeds This image is a reminder to tap to take a snapshot of the page after you have entered your response.

6 ...Background The chemical reaction for cellular respiration is: C 6 H 12 O 6 + 6O > 6CO 2 + 6H 2 O + Energy (ATP molecules) CO 2 is a measurable end product of the reaction. The amount of CO 2 created indicates the rate of cellular respiration occurring inside an organism. The process of cellular respiration occurs in the mitochondria of all eukaryotic cells. Respiration of Germinating Seeds

7 Self-Check 2.Why does this lab involve measuring the amount CO 2 produced by seeds? a)indicates rate of velocity b)indicates rate of mortgage interest c)indicates rate of cellular respiration d)indicates heart-rate Respiration of Germinating Seeds

8 (Your teacher may have already completed these steps for you) 24 hours prior to the lab: Place 20 dry seeds in a beaker and fill the beaker with tap water. Put the beaker in a dark location for 24 hours. The seeds will begin to germinate. 30 minutes prior to the lab: Place half of the germinating seeds into an ice water bath. Allow them to cool before starting the experiment. Pre Lab Set Up Respiration of Germinating Seeds

9 Safety Use all standard laboratory safety procedures. DO NOT allow the Carbon Dioxide Gas Sensor to get wet. Respiration of Germinating Seeds

10 Materials and Equipment PASPORT Carbon Dioxide Gas Sensor PASPORT Extension cable Sampling bottle (included with sensor) 1 Beaker (1000 mL) 10 dry seeds Collect all of these materials before beginning the lab. Ice (cubes or crushed) Paper towels Tap water 10 germinating seeds at room temperature 10 germinating seeds in ice water CO 2 Gas Sensor PASPORT Extension Cable Respiration of Germinating Seeds

11 Sequencing Challenge A. Analyze the data. B. Measure the CO 2 produced by dormant seeds. C. Measure the CO 2 produced by germinating seeds. D. Measure the CO 2 produced by cold seeds. Respiration of Germinating Seeds The steps to the left are part of the procedure for this lab activity. They are not in the right order. Determine the correct sequence of the steps, then take a snapshot of this page.

12 Q1: Why is it important to firmly insert the sensor into the bottle and create a seal? Setup: Dormant Seeds 1.Connect a PASPORT extension cable to the SPARK Science Learning System. Plug the CO 2 Gas Sensor in the cable and then press the green button on the sensor to calibrate it. 2.Place 10 dormant (dry) seeds into the sampling bottle. 3.Put the end of the CO 2 Gas Sensor into the sampling bottle. Firmly plug the end of the sampling bottle with the rubber stopper. Do not let the bottle tip over! Respiration of Germinating Seeds

13 Q2: What will happen to the carbon dioxide level inside the bottle? Draw your prediction in the graph.* *To Draw a Prediction: 1.Tap to open the tool palette. 2.Tap then use your finger to draw your prediction. 3.Tap when finished. 4. If you make a mistake, tap to clear your prediction. Respiration of Germinating Seeds

14 Q3: Why do you need to rinse out the sample bottle? Collect Data: Dormant Seeds Note: Avoid jarring the sample bottle during data collection, as this can cause erratic results. 1.Tap to begin collecting data. 2.Record data for 10 minutes, then tap to end data collection. 3.Remove the sensor and the dormant (dry) seeds from the sample bottle. 4.Rinse the sample bottle out with tap water. Continue to the next page. Respiration of Germinating Seeds

15 Q4: What happened to the amount of CO 2 produced by the dry seeds over time? Respiration of Germinating Seeds

16 Q5: What will happen to the CO 2 level when germinating seeds are placed in the bottle? Draw your prediction in the graph.* Respiration of Germinating Seeds *To Draw a Prediction: 1.Tap to open the tool palette. 2.Tap then use your finger to draw your prediction. 3.Tap when finished. 4. If you make a mistake, tap to clear your prediction.

17 Collect Data: Room-Temp Seeds 1.Place ten of the room-temperature germinating seeds into the sample bottle. 2.Re-insert the sensor into the bottle. 3.Tap to begin collecting data. 4.Record data for 10 minutes, then tap to end data collection. 5.Remove the sensor and the dormant (dry) seeds from the sample bottle. 6.Rinse the sample bottle out with tap water. Respiration of Germinating Seeds

18 Q6: What happened to the amount of CO 2 produced by the germinating seeds over time? Respiration of Germinating Seeds

19 Q7: How did your predicted graph line compare with the actual line? Respiration of Germinating Seeds

20 Q8: What will happen to the CO 2 level when germinating seeds are placed in the bottle? Draw your prediction in the graph.* Respiration of Germinating Seeds *To Draw a Prediction: 1.Tap to open the tool palette. 2.Tap then use your finger to draw your prediction. 3.Tap when finished. 4. If you make a mistake, tap to clear your prediction.

21 Collect Data: Chilled Seeds 1.Fill the 1000 mL beaker with ice and water. 2.Place ten of the chilled germinating seeds into the sample bottle. Place the bottle into the icy beaker so the seeds stay cold! 3.Re-insert the sensor into the bottle. 1.Tap to begin collecting data. 2.Record data for 10 minutes, then tap to end data collection. 3.Remove the sensor and the chilled seeds from the sample bottle. 4.Rinse the sample bottle out with tap water. Respiration of Germinating Seeds

22 Q9: What happened to the amount of CO 2 produced by the chilled germinating seeds over time? Respiration of Germinating Seeds

23 Q10: How did your predicted graph line compare with the actual line? Respiration of Germinating Seeds

24 * To Find the Difference Between Two Data Points: 1.Tap to open the tools palette. 2.Tap and then tap two points on the data run. 3.Adjust using both buttons and then tap 4.Tap to display the differences. Data Analysis 1.Determine the change in CO 2 concentration for all runs, and record the information in the data table on the next page. Respiration of Germinating Seeds

25 Data Analysis 2.Record the initial, final, and change in CO 2 concentration for all runs, in the data table to the left. *To Enter Data into a Table: 1.Tap to open the tool palette. 2.Tap then tap a cell in the data table to highlight it in yellow. 3.Tap to open the Keyboard screen. Respiration of Germinating Seeds

26 Analysis 1.How does the rate of CO 2 production for dormant seeds compare to the rate of CO 2 production for room-temperature germinating seeds? What does this information indicate? Respiration of Germinating Seeds

27 2.How does the rate of CO 2 production for room-temperature geminating seeds compare to the rate of CO 2 production for chilled germinating seeds? What does this information indicate? Respiration of Germinating Seeds Analysis

28 3.How did your predictions compare to the actual data collected? What did you learn? Respiration of Germinating Seeds Analysis

29 Synthesis 1.If cellular respiration occurs during seed germination, what would happen to CO 2 levels if you added water to a sample bottle filled with dry peas? Respiration of Germinating Seeds

30 2.What is the chemical equation for cellular respiration? Where does this process occur in the cell? Respiration of Germinating Seeds Synthesis

31 3.What gaseous molecule is released during cellular respiration? Respiration of Germinating Seeds Synthesis

32 4.Explain why plants need to perform cellular respiration even though they are photosynthetic. Respiration of Germinating Seeds Synthesis

33 5.What environmental factors can affect the rate of cellular respiration in an organism? Respiration of Germinating Seeds Synthesis

34 Multiple Choice 1.Cellular respiration takes place in what part of the cell? a)chloroplasts b)nuclei c)lysosomes d)mitochondria Respiration of Germinating Seeds

35 2.In cells, the energy stored in glucose is used to make what energy rich compound? a)water b)ATP c)ADP d)DNA Respiration of Germinating Seeds Multiple Choice

36 a) 5-10 minutes b) seconds c) seconds d) minutes 3.According to the graph, the rate of CO 2 production was highest at what time? Respiration of Germinating Seeds Multiple Choice

37 You have completed the lab. Congratulations! Please remember to follow your teacher's instructions for cleaning-up and submitting your lab. Respiration of Germinating Seeds

38 Images are taken from PASCO documentation, public domain clip art, or Wikimedia Foundation Commons References Respiration of Germinating Seeds


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