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Chapter 9: Cellular Respiration and Fermentation.

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Presentation on theme: "Chapter 9: Cellular Respiration and Fermentation."— Presentation transcript:

1 Chapter 9: Cellular Respiration and Fermentation

2 Essential Knowledge  2.a.1 – All living systems require constant input of free energy ( ).  2.a.2 – Organisms capture and store free energy for use in biological processes ( ).

3 Cellular Respiration - Preview  Def - The process of releasing energy/ATP from food  Food - Stored energy in chemical bonds (provides fuel)  ATP - Useable energy for cellular processes  Wastes – CO 2 and H 2 O  Mitochondrion store most of equipment needed for rxn

4 Respiration (Rs) - Equation C 6 H 12 O O 2 6 CO H 2 O + energy (ATP or heat)  Rxn is spontaneous (- ∆ G)  The energy is released (exergonic) from the bonds in the org molecules Remember: Org molecules store energy in their arrangement of atoms Org molecules can be carbs, proteins or fats/lipids


6 Focus of Chapter Cellular Rs 1. Purpose - what is the reaction suppose to do for the cell? 2. Location - where does it occur? 3. Requirements - what is needed to make it run? 4. Products - what does it produce? Other Fermentation, Redox

7 Fuel? What is used?  Organic molecules with a large amt of hydrogen make great fuel! Why? H becomes oxidized (only has one e-) very easily and energy is released Remember: Carbs, fats, proteins are storage bins for e- associated with hydrogen

8 Oxidation - definitions  Loss of electrons  Loss of energy  Loss of hydrogens from carbons  Ex: Na+ (of NaCl)

9 Food and Oxidation  Food (organic molecules) contain a lot of H atoms These serve as great long-term fuels Why? ○ Because H becomes easily oxidized (releases energy frequently)

10 Reduction - definitions  Gain of electrons (REDUCING + charge)  Gain of energy  Gain of hydrogens to carbons  Ex: O is often reduced! Why? Because electrons are pulled closer to O

11 Redox reactions

12 Equation for Rs C 6 H 12 O O 2 6 CO H 2 O + energy (ATP/heat) General Redox Equation: Xe- + Y  X + Ye- Reduced Oxidized

13 Redox reactions  Involves transfer of e- and energy release Sometimes doesn’t involve complete transfer  Red and Oxd reactions are usually paired or linked together. Why?Because e- transfer requires donor and acceptor  Many of the reactions will be done by phosphorylation  Redox video Redox video

14 Phosphorylation  Adding a phosphate group to a molecule Ex: ATP cycle (add P to ADP = ATP)  Two types: Oxidative AND substrate-level  The phosphate group adds “energy” to the molecule for chemical reactions (think ATP cycle) Endergonic rxn

15 Phosphorylation

16 Cell Respiration – 3 parts 1. Glycolysis 2. Krebs Cycle 3. Electron Transport Chain **Use page 167 as a starting point: Cellular Respiration - A Preview


18 Glycolysis  Glyco- glucose -lysis: to split  Formula for glucose: C 6 H 12 O 6  Universal step in all Rs types.  Likely the earliest type of cell energy processes  Overview: Glucose splits into 2 3-C sugars (then oxidizes to form pyruvate)

19 Glycolysis  Function - To split glucose and produce NADH and ATP ATP made by substrate-level phosphorylation ○ Enzyme transfers phosphate group from substrate/reactant to ADP to make ATP  Location – Cytoplasm of the cell


21 Electron Carrier Compounds  Molecules that transport or shuttle electrons within the cell  Exist in two forms: Oxidized (ox) Reduced (red)  Ex: NAD and FAD

22 NAD  Nicotinamide Adenine Dinucleotide  NAD e - NADH  NAD + = oxidized form  NADH = reduced form* *Reduced by e- from food oxidation


24 Glycolysis Requirements  Glucose  2 ATP  4 ADP  2 NAD +  Can occur with or without O 2

25 Glycolysis - Products  2 Pyruvic Acids (a 3-Carbon acid)  2 ADP, 4 ATP, 2 NADH  NET RESULT: 2 ATP per glucose 2 NADH 2 pyruvate H 2 O Notice: No CO 2 made during this step! Glycolysis Intro


27 Krebs Cycle  Oxidizes fuel from pyruvate molecules Remember? Pyruvate formed during glycolysis  Also called: Citric Acid Cycle Tricarboxylic Acid Cycle

28 Krebs Cycle  Function: Oxidize pyruvic acid (to make CO 2 )  Produces: NADH and FADH 2  Location: Mitochondria matrix  Before Krebs: Acetyl CoA must be formed Acetyl CoA is needed to actually start Krebs

29 Pyruvate moved into mito?  Why? How? Pyruvate is moved into mitochondria (from cytoplasm) Why? This is where the 2 nd step occurs (specific enzymes are in mito) Serves as a checkpoint Uses active transport and transport proteins. ○ Why? Pyruvate is a charged molecule!

30 Formation of Acetyl CoA

31 Krebs Cycle Requirements  Pyruvic acid (3C acid)  Acetyl coenzyme A  4 NAD +  1 ADP  1 FAD  Double this list for each glucose

32 Krebs Cycle Products  3 CO 2  Acetyl CoA  4 NADH  1 FADH 2  1 ATP  Double this list for each glucose Made from pyruvate LOADS of energy stored in these molecules Krebs Cycle Intro


34 Krebs Cycle notes  Notice: Only 1 ATP made per cycle Produces most of the cell's energy in the form of NADH and FADH 2  Does NOT require O 2

35 Comment about ATP  The ATPs produced directly in Krebs Cycle and Glycolysis are by: Substrate-level phosphorylation  The P group is transferred from a substrate to ADP Making ATP

36 At this point…  After the Krebs and glycolysis cycles, the cell has made a total of 4 ATP. Remember: some ATP had to be used to power the cycles.  Most energy (at this point) comes from NADH and FADH 2

37 Electron Transport System  ETC/S or Electron Transport Chain  This is a collection of proteins that are structurally linked  Located in inner membrane of mito Folding of mito (cristae) allows for lots of places (large surface area!) for ETC to occur

38 ETC/S  Uses sets of Cytochromes Fe (Iron)-containing proteins to pass electrons  The Cytochromes alternate between Red and Ox forms and pass electrons down to O 2 Remember: LEO, GER; LEO the lion goes GER Losing Electrons is Oxidation; Gaining Electrons is Reduction

39 As e- moves down the ETC, free energy decreases

40 ETC/S  Function: Convert NADH and FADH 2 into ATP  Location: Mitochondria cristae/folds

41 ETC Requirements  NADH or FADH 2  ADP O2O2 We finally see the need/requirement of Oxygen

42 ETC Products  NAD + and FAD  ATP (LOTS!!!) H2OH2O Remember: Water was also produced during glycolysis ETC explanation

43 ETC - ATP Yields  Each NADH  3 ATP  Each FADH 2  2 ATP 34 ATP  TOTAL: 34 ATP

44 Chemiosmotic Hypothesis  ETC energy is used to move H + (protons) across the mito/cristae membrane  ATP is generated as the H + diffuse back into the matrix


46 ATP Synthase Enzyme  An enzyme that uses the flow of H + to make ATP  Works like an ion pump in reverse, or like a waterwheel under the flow of H + “water”  Power source: H+ concentration difference on opposite sides of mitochondrial membrane

47 Oxidative Phosphorylation  ATP synthase uses oxidative phosphorylation to make ATP during ETC  Uses H ions to make ATP and water (using Oxygen)

48 ATP Synthase

49 Alcoholic Fermentation  Done by yeast A kind of fungus  Used in brewing beer, winemaking, and baking CO 2 bubbles generated give: ○ Bread a rising effect ○ Wine/Beer the carbonated effect

50 Alcoholic Fermentation  Uses only Glycolysis  An incomplete oxidation - energy is still left in the products (alcohol)  Does NOT require O 2  Produces ATP (when O 2 is not available)

51 Alcohol

52 Lactic Acid Fermentation  Uses only Glycolysis  An incomplete oxidation - energy is still left in the products (lactic acid)  Does NOT require O 2  Produces ATP (when O 2 is not available)

53 Lactic acid

54 Lactic Acid Fermentation  Done by human muscle cells under oxygen debt Lactic Acid is a toxin and can cause soreness and stiffness in muscles Oxygen intake can’t keep up with sugar breakdown  Used in dairy industry (yogurt/cheese)  Also used to produce methanol and acetone

55 Fermentation - Summary  Way of using up NADH so Glycolysis can still run  Provides ATP to a cell even when O 2 is absent

56 Fermentation *Alcoholic OR *Lactic acid

57 Aerobic vs Anaerobic  Aerobic - Rs with O 2  Anaerobic - Rs without O 2  Aerobic - All three Rs steps  Anaerobic - Glycolysis only

58 Strict vs. Facultative  Strict - can only do Rs this one way Either aerobic OR anaerobic-NOT both!  Facultative - can switch types depending on O 2 availability Ex - yeast

59 Question??  Since yeast can do both aerobic and anaerobic Rs, which is the better process if given a choice? Hint: Check the ATP yields from both processes.

60 ATP yields by Rs type  Anaerobic - Glycolysis only gets 2 ATPs per glucose  Aerobic - Glycolysis, Krebs, and ETC. Generates many more ATPs per glucose.

61 Aerobic ATP yield  Glycolysis - 2 ATPS, 2 NADHs  Krebs - 2 ATPS, 8 NADHs, 2 FADH 2  Each NADH = 3 ATP  Each FADH 2 = 2 ATP

62 Aerobic ATP Sum  10 NADH x 3 = 30 ATPs  2 FADH 2 x 2 = 4 ATPs  2 ATPs (Gly) = 2 ATPs  2 ATPs (Krebs) = 2 ATPs  Max = 38 ATPs per glucose

63 However...  Some energy (2 ATP) is used in shuttling the NADH and pyruvate from Glycolysis into the mitochondria  Actual ATP yield ~ 36/glucose

64 Yeast  Would rather do aerobic Rs; This has 18x more energy per glucose than anaerobic  But, anaerobic will keep you alive if oxygen is not present.

65 Importance of Rs  Convert food to ATP Living orgs use ATP to fuel body processes Ex: reproduction, cell division  Provides materials for use in other cellular pathways

66 Other Respiration Items of Importance  Alcohol Industry - almost every society has a fermented beverage  Baking Industry - many breads use yeast to provide bubbles to raise the dough


68 Alcohol Matching Game! Sugar Cane Gin Barley Rum Grapes Wine Juniper Cones Vodka Agave Leaves Beer Rice Tequila Potatoes Saki

69 Summary  Identify the basic chemical equation for cellular respiration.  Identify the main reaction sequences of cellular respiration.  Recognize the location, function, requirements, and products, for each cellular respiration reaction.  Recognize and be able to discuss the chemiosmotic model for ATP generation.  Recognize the reactions and importance of fermentation.  Contrast and compare aerobic and anaerobic respiration.  Identify the biological and commercial importances of respiration.

70 Exclusion Statements  You do NOT need to memorize the steps in glycolysis and the Krebs cycle, the structures of the molecules, or the names of the enzymes that are involved.  You do NOT need to memorize the names of the specific electron carriers in the electron transport chain (ETC).

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