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BIO 225 Microbiology Ken Malachowsky Florence-Darlington Technical College.

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Presentation on theme: "BIO 225 Microbiology Ken Malachowsky Florence-Darlington Technical College."— Presentation transcript:

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2 BIO 225 Microbiology Ken Malachowsky Florence-Darlington Technical College

3 MICROSCOPE Magnifies Illuminates Contrasts Resolves Optics SPECIMEN Distinguishes Lens systems Condenser OCULAR OBJECTIVE Total magnification Multiply two powers Lamp Vary Light intensity Iris diaphragm BackgroundAbility to Distinguish Provide sharp, crisp image Two close objects

4 Leewenhoek’s Simple Microscope

5 A Compound Brightfield Microscope

6 Scanning Power X 4x

7 Low Power X 4x X 10x

8 High Power X 4x X 10x 40x 4x 10x 40x 100x Field of Vision

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10 Depth Perception Steve Berg winona state university, used with permission

11 Refraction

12 The use of Immersion Oil

13 Microscope Rules Never force anything on the microscope Inform the instructor if there is a problem with the microscope Only use your assigned microscope Please do the following when you are finished –Click the 4x objective in place –Lower the stage far from the objective –Place the microscope in its assigned space

14 Slides to View Letter E Colored Threads Cheek cells Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wetmount KM

15 Cheek Cells 100x400x KM

16 Slides to View Letter E Colored Threads Cheek cells Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wetmount KM

17 Cyclops 100x KM

18 Daphnia 100x KM

19 Volvox 100x400x KM

20 Spirogyra 400x KM

21 Amoeba 100x 400x KM

22 Paramecium 100x 400x KM

23 Representative algae, or plantlike protists: The diatom Campylodiscus hibernicus These protists usually have cell walls surrounded by a loosely attached test that contains silicon or calcium carbonate. Most reproduce by binary fission. Some lack flagella.

24 Diatoms 400x KM

25 1000x Mixed Bacteria

26 Slides to View Letter E Colored Threads Cheek cells Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wet mount


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