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Fluorescence Microscopy Test your prediction skills.
Hoechst stain The structure of these stains causes them to slide in between the bases of DNA and fluoresces blue. –What structure of the cell would you predict would be stained?
Human cheek cell We’ll come back to the green.
Connective tissue cells – fibroblasts We’ll come back to the red and green.
Bull sperm The heads of sperm are filled completely with this structure. We’ll come back to the green.
DiOC6 associates with lipids and produces a green color What subcellular structures are lipid based?
Human cheek cell
Ovary cells from Chinese Hamster
Oxidation revealer red Concentrated in the organelle that uses oxygen to generate ATP
Connective tissue cells - fibroblasts
Oxidation revealer green Same characteristics as oxidation revealer red, but a different color fluorescence
oxidation revealing green same structure, highly elongated shape Fathead minnow cell
The tails of sperm are packed with these structures.
acid loving red Accumulates in organelles with an acid pH, useful for breaking down wastes
Canine kidney cells We’ll come back to the green, which is a different dye from the one you’ve seen already.
BODIPY ceramide (green) Ceramides are molecules taken up and metabolized by the organelle responsible for for transporting lipids and proteins to the cell membrane
Canine kidney cells
Phalloidin green Binds to filamentous actin
Phalloidin red Binds to filamentous actin
Bovine pulmonary artery endothelial cells – the cells lining the interior of the major vessel bringing blood into the lungs. In this picture, the green is a microtubule stain. The red is phalloidin.
MCB 130L Part 2 Lecture 3 Transfection and Protein localization.
DO NOW: What is the job of the cytoplasm? Write the equation for cellular respiration: Put the following in order from smallest to largest: tissue, organ.
The Circulatory System. What is the Purpose The circulatory system transports needed materials throughout out body including nutrients and gases (oxygen)
Lab 5A and 5B Overview Investigating protein sorting signals using cloning, transfection, GFP-fusion proteins, and vital stains for cellular compartments.
Topic: Final Exam Review Aim: Let’s review Cells and Cell Processes Do Now: Describe the difference between independent and dependent variables. HW: Human.
TODAY’S AGENDA P.O.D: Make an initial observation of the fish tank before the food coloring is added. Then predict what will happen when a fish tank filled.
Cell Membrane and Transport Maintaining homeostasis and providing nutrients to cells.
Human Body Assessment How Does Cellular Respiration Work?
The Circulatory System - Also known as the Cardiovascular system. Parts: 1. Heart 2. Blood 3. Blood Vessels.
Today: Circulation and Respiration Exam 2 T 4/5 in class (bring cheat sheet) Take-home 2 due 4/5 in class.
Cytoskeleton & Cell Membranes: 3.2B Cytoskeleton & Cell Membranes.
Cell Organelles and features Chapter 4. Plasma Membrane Also called cell membrane Allows certain molecules to enter and exit a cell It separates internal.
The Cell Part 1. Prokaryotic Cells Prokaryotic cell: One celled organisms Lack membrane bound nucleus and organelles Contains Cytoplasm
Project: Protein Localization in Mammalian Cells Idea: Compare localization of proteins (ZFP568 & GALT) in two types of mammalian cells Significance: Protein.
Cell Theory Standard: 7.1 All living organisms are composed of cells, from just one to many trillions, whose details usually are visible only through a.
Warm Up What are the necessities of life? In other words, what does a living organism need to survive?
Regents Review Topic 1: Similarities and Differences Among Living Organisms.
Warm-Up organelles 11/8/11 Why would plants need to have both chloroplasts and mitochondria? (Look at function for each) Have out animal and plant drawings.
The human circulatory system This system is one of the most important in the body. It is made up of 3 main parts: The heart The Lungs The blood vessels.
Chapter 3a Compartmentation: Cells and Tissues. About this Chapter Body compartments Biological membranes Intracellular compartments Tissue types and.
CHAPTER 6: CELLS General. Cells: General CHAPTER 6: CELLS Compartments.
Cell Diversity Tissue types Tissue Functions.
Chapter 2 p. 52/53 1. All living things are _____________________________________. 2. A cell is ___________ and __________ unit of life. 3. All cells.
Objectives 1. Describe and explain the three parts of the cell theory 1. Describe and explain the three parts of the cell theory 2. Determine why cells.
Cell Review. The Needs of a Cell Name 2 things animal cells need to take in: ___________________________ oxygen, amino acids, sugar, water Name 2 things.
7 th Grade Cells Review. Explain one exception to the cell theory. Viruses – are not made up of cells.
POWERPOINT ® LECTURE SLIDE PRESENTATION by ZARA OAKES, MS, The University of Texas at Austin Copyright © 2007 Pearson Education, Inc., publishing as Benjamin.
What are cells? An organism is made of organ systems. Organ systems are made up of organs Organs are made up of tissues Tissues are made up of.
Circulatory System. Functions of the Circulatory System Blood carries oxygen and nutrients to the cells in the body Blood picks up carbon dioxide and.
Ch 7 - Cell Structure & Function The fundamental life processes of plants and animals depend on a variety of chemical reactions that occur in specialized.
Cell Structure & Function BINGO A Review of Cellular Organelles.
THE CELL Cells are mainly formed of water, but present in their structure are proteins, carbohydrates, and nucleic acids (DNA). Structure Cell membrane.
Unit 3. Smallest unit of living organisms Four basic parts of the cell Cell membrane – encloses the cell Nucleus – houses genetic material Cytoplasm-
Chapter 3. Encloses the cell and forms a boundary Separates the interior of the cell from its surroundings Interior is filled with cytoplasm
A fluorophore is a molecule that can absorb light at a one wavelength and re-emit at a slightly longer wavelength. Examples are cyanine dyes – Cy3 and.
Special Staining (I). Carbohydrates 1- PERIODIC ACID SCHIFF'S (PAS ) Purpose: Glycogen is present in liver, kidney, skeletal and cardiac muscle. The.
Cytoskeleton Lab 2: The Effects of Cytochalasin B on the Actin Cytoskeleton & Cell Viability in Mouse Fibroblast Cells.
Lecture 3 BIO 344. Hydrophobic amino acids in green and yellow.
The key to every biological problem must finally be sought in the cell, for every living organism is, or at some time has been, a cell. E.B. Wilson, 1925.
EOC Review: Biochemistry, Enzymes, Cell, and Cell Transport Christopherson.
Kingdom Animalia The Invertebrates (95%) Sunflower Starfish Oyster Blue Morpho (Costa Rica) Earthworm Whip Coral Scorpion.
Eukaryote Cells Eukaryote Structure Overview: Microfilaments, Microtubules, Intermediate Filaments Flagella & Cilia Nuclear Structure & Cell Cycle Endoplasmic.
Arteries, Veins and Capillaries Chapter 15. The Cardiorespiratory System Includes function of the heart, blood vessels, circulation, and gas exchange,
Cell Membrane and Tonicity Worksheet. Composition of the Cell Membrane The cell membrane is also called the plasma membrane and is made of a phospholipid.
Lets take a look at typical animal cells. Nucleus Cytoplasm Membrane Animal Cells.
Cells and Tissues Chapter 3. Cells and Tissues Carry out all chemical activities Cells are the building blocks of all living things Tissues are groups.
Similarities and Differences Among Living Organisms.
Proteins serve a variety of functions. Transport –Myoglobin transports O 2 throughout muscles. –Hemoglobin transports O 2 in blood. Structural –Actin forms.
Cell Motility and Shape require microfilaments (F-actin), microtubules and intermediate filaments. Not surprisingly, the actin skeleton is dynamic, not.
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