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Extraction of DNA Lab #6. Step 1: Extract DNA from Banana Follow the instructions listed on your handout. (Steps # 1-9) Materials on the cart: Amt you.

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Presentation on theme: "Extraction of DNA Lab #6. Step 1: Extract DNA from Banana Follow the instructions listed on your handout. (Steps # 1-9) Materials on the cart: Amt you."— Presentation transcript:

1 Extraction of DNA Lab #6

2 Step 1: Extract DNA from Banana Follow the instructions listed on your handout. (Steps # 1-9) Materials on the cart: Amt you need -Shampoo1 teaspoon -Salt 2 pinches -Vial w/chilled 91% alcohol1 vial/pair -De-ionized water20 ml -Banana mixture3 teaspoons **Important notes & Reminders: -Note the appearance & describe what you see. -Rinse out your vials after you have made your observations -Answer questions # 1-4

3 Follow up Lab: Extracting Your DNA Supplies are in the back of the room. 1) Pick up a dixie cup w/ salt water (2 tsp) **TWO teaspoons salt in one quart (liter) Dist. water 2) Swish around in your mouth thoroughly for 30 sec. 3) Spit back into the cup 4) Pour the contents of the cup into the vial with soap solution (Woolite or Palmolive 3:1 ratio - water & soap) 5) Gently rock the vial back & forth (3 min) 6) Take one pipette full of chilled alcohol (95% Ethanol) and slowly add it to the vial while tilted making sure that they don’t mix. 7) Let stand for 1 minute 8) Gently mix the alcohol into the soap layer with a glass rod. 9) DNA should precipitate (whitish) –Be patient

4 Summary of Results –Due: Fri. 11/19 Title: “Banana vs. Your DNA Introduction/Background Separation of DNA: Explain the process -Why does this technique work? -Why do you need salt, shampoo, & alcohol? Results: Explain what you observed –Describe what you saw –How was the Banana DNA different from your DNA? –How could you verify that what you have is DNA? Conclusion/Evaluation: –How could this lab activity be improved? (Sources of error) –What else could be used? Other techniques?


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