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Laboratory Diagnosis Microscopic ex Virus cultivation Serological tests Detection of N/A.

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Presentation on theme: "Laboratory Diagnosis Microscopic ex Virus cultivation Serological tests Detection of N/A."— Presentation transcript:

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2 Laboratory Diagnosis Microscopic ex Virus cultivation Serological tests Detection of N/A

3 Virus cultivation   Laboratory animal  Embryonated egg  Cell culture

4 Embryonated Egg System

5 1) Chorioallantoic membrane inoculation CAM Allantoic Fluid Yolk sac Amniotic Fluid Air sac Albumin

6 2) Allantoic inoculation Yolk sac Allantoic fluid CAM Air sac Amniotic Fluid

7 3) Amniotic inoculation Air sac Yolk sac Amniotic Fluid Allantoic Fluid CAM

8 4) Yolk sac inoculation Air sac Yolk sac Albumin

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11 Cell culture No of sub passages Primary C/C 1 or 2 1 or 2 Diploid C/C [semicontinuous] 20 to to 50 Continuous cell line Indefinite Indefinite

12 Variation in Sensitivity of cell cultures to infection by viruses commonly isolated in clinical virology laboratories Virus Cell culture a PMK HDF HEp-2 RNA virus DNA virus Enterovirus Rhinovirus Influenza virus RSV Adenovirus HSV VZV CMV / PMK, primary MK. Degree of sensitivity: +++, highly sensitive;++, moderately sensitive; +, low sensitivity; +/-, variable; -, not sensitive

13 VIRAL CULTURE PROCDURE  Processing of the specimen  Inoculation onto the C/C  Maintenance of the inoculated C/C  Detection of viral growth

14 VIRAL CULTURE PROCDURE  Processing of the specimen

15 Inoculation onto the C/C

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19 Detection of viral growth  Cytopathic effects  Comprise;  Rounding, shrinkage, aggregation & loss of adherence & loss of adherence  Giant cell formation  Inclusion bodies formation

20  Rounding, shrinkage, aggregation & loss of adherence & loss of adherence Uninfected ccCell rounding

21 Syncytium formation Uninfected ccCell roundingSyncytium

22 Inclusion bodies formation Inclusion bodies formation

23 IDENTIFICATION  Presumptive ID  Characteristic CPE  Type of cells  Time taken  Final ID  IF

24 Detection of viral replication  CPE  Hemadsorption  Interference  EM

25 Not infected cells Vero cells McCoy cells Human diploid fibroblast

26 Cytopathic effects  Intracytoplasmic inclusion bodies  Intranuclear inclusion bodies  Giant cell formation

27 Cytopathic effects  Vero cells infected with Measles virus stained by H & E  Intracytoplasmic inclusion bodies

28 Cytopathic effects  Vero cells infected with Measles virus stained by H & E  Giant cell formation

29 Cytopathic effects  Vero cells infected Herpes viruses stained by H&E  Intranuclear inclusion bodies

30 Not-infected Not-infected CC

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32 Giant cell formation

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35 Intranuclear inclusion bodies

36 عن أبي هريره رضي الله عنه عن أبي هريره رضي الله عنه أن رسول الله صلي الله عليه وسلم قال : أن رسول الله صلي الله عليه وسلم قال : { كلمتان خفيفتان على اللسان, ثقيلتان في الميزان حبيبتان إلي الرحمن : { كلمتان خفيفتان على اللسان, ثقيلتان في الميزان حبيبتان إلي الرحمن : سبحان الله وبحمده, سبحان الله وبحمده, سبحان الله العظيم } سبحان الله العظيم }

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