Mathematical Model: Total Magnification = Ocular Lens magnification x Objective Lens magnification Sheila has an ocular lens with 5x written on it and a scanning lens of 5x. How much larger is her image than the original specimen? If Sheila switches to low power which has 10x written on it, how much larger will her image be? What is the new magnification?
1millimeter = 1000 micrometers What is the symbol for millimeters? What is the symbol for micrometers? Light is composed of frequencies of energies that have their wavelengths measured in nanometers. How is a nanometer related to a micrometer?
Determine field of view under low power. Convert millimeters to micrometers Estimate size of image by determining the percent coverage of the field of view. Multiply the percentage of coverage by the field of view.
Determine the field of view in mm under low power. Convert the field of view from mm to um. Determine the field of view under high power using the mathematical model below Field of view high power = low power magnification x field of view under low power high power magnification Estimate the amount of coverage (or percentage) of field of view for that specimen. Multiply the amount of coverage by the field ov view under high power.
1. Suppose the field of view under scanning is 6 mm across and has a magnification of 50x. What will the field of view be under low power which is 100x? 2. Suppose a specimen takes up 1/3 the field of view under low power (100x) which has a field of view of 1.5 mm, how large is the specimen in micrometers? 3. A microscope has a field of view of 2 mm under low power (100x). A specimen takes up 75% of the field of view under high power (430x). How large is the specimen in micrometers? 4. A microscope has a field of view of 1.75 mm under low power (100x). If 5 specimens can fit across the field of view under high power (400x), how large is the specimen in micrometers?
Low power 100x has field of view of 1.5 mm. Viewed under high power with magnification of 430x What is the diameter of field of view under high power? What is the approximate size of the elodea cell?