Presentation is loading. Please wait.

Presentation is loading. Please wait.

Prepared by Mrs / Hamdia Mohammed. At the end of this lecture each student should be able to: 1. Define purposes of a specimen collection. 2. Explain.

Similar presentations

Presentation on theme: "Prepared by Mrs / Hamdia Mohammed. At the end of this lecture each student should be able to: 1. Define purposes of a specimen collection. 2. Explain."— Presentation transcript:

1 Prepared by Mrs / Hamdia Mohammed

2 At the end of this lecture each student should be able to: 1. Define purposes of a specimen collection. 2. Explain the different type of specimen collection. 3. Identify the universal precautions.

3 6. List necessary information included in laboratory request form. 7. Identify nursing responsibilities associated with specimen collection

4  Assist in the diagnosis of pathological conditions.  Evaluate the client’s progress and to regulate treatment.  Prevent or reduce the severity of disease.

5 Rule 1: - Consider ALL patients potentially infectious. Rule 2: - Assume ALL blood, body fluids and tissues are contaminated. Rule 3: - Assume ALL used needles or other sharps are contaminated.

6 1- Urine 2- Blood 3- Stool 4- Sputum 5- Wound culture sample

7  Random specimen.  Midstream specimen.  Sterile specimen.  24-Hour urine collection.  Supra pubic needle aspiration of the bladder.

8 A random routine urine specimen can be collected with a client voiding naturally. Uses:- * Routine Urine analysis testing * PH * Glucose level

9 - The client’s void into a clean urine cup, urinal, or bedpan. - Client’s drinks a glass of fluid before the procedure about 30 minute. - A client should be voided before defecating.

10 A - Bedpan, fracture pan and Urinal containers for male patient used to collect urine from non ambulatory patients B - Specimen hat : container that is placed interiorly on the toilet, underneath the seat. Used to collect urine. Specimen cup container

11 - Placing bedpan against buttocks while patient is on his side. - Patient raising self in bed for bed pan.

12 to obtain a specimen relatively free of the microorganisms growing in the lower Urethra. Uses Urine culture

13 - Cleansing of the external genitalia. - Allowing the initial portion to escape. - The clients collects the specimen during the middle portion of voiding. - Remove specimen container before flow of urine stops and the client finishes voiding into bedpan or toilet.

14 Obtaining urine specimen from an indwelling catheter. Uses - Urine culture. - Specific gravity: (urine concentration or the amount of solutes metabolic wastes and electrolytes) present in urine.

15 ( A) First, use a swab moistened with an antiseptic to clean the area where the sterile needle will be introduced. (B) Then insert the needle and withdraw a specimen of urine.

16  Washes hands and wear gloves.  Uses a sterile syringe to withdraw urine.  Urine specimen is not collected for culture from a urine drainage bag.

17 Purpose - To assess the ability of the kidney to concentrate and dilute urine. - To determine disorders of glucose metabolism.

18 Indication :  Patient with UTI.  In neonate.  Young infants.  Patient whom catheterization is contraindicated.  Suspected anaerobic bacteriuria.

19 TThe client’s should have a full bladder. PProperly disinfected of the skin. 119 or 20 gauge needle is passed through the skin into the bladder. UUrine is aspirated into a syringe

20  Wash hands  Confirm the identity of the patient  Explain the procedure  Assemble all necessary equipment  Put the patient in comfort position

21  Check both arms to select the larger and fuller veins.  Apply the tourniquet.  Ask the patient to open and close his/her fist so their veins become prominent.  Clean the venipuncture site with the alcohol swab in a circular motion.

22  Insert the stopper of the first tube to be drawn into the adaptor.  Insert the needle into the vein with the bevel facing upward.  Fill the tube until the vacuum is exhausted.  Place a cotton ball or 4 x 4 square piece of gauze over the site.

23  Remove gloves and dispose of in a properly identified biohazard bag or container.  Wash hands thoroughly after phlebotomy.  verify that the information on the sample tubes match is the requisition form

24  Used to detect bacteria or fungi in the systemic circulation.  Performed by sampling peripheral blood using a no-touch or aseptic venipuncture method.  At least 10ml blood should be withdrawn and dispensed into two blood culture bottles, one for aerobic and one for anaerobic culture.

25 Purpose: 1- To determine the presence of occulted blood for presence of (ulcers, inflammatory disease or tumors). 2- To analyze for dietary products. 3- To detect the presence of ova and parasites. 4- To detect the presence of bacteria or viruses.

26  Defecate in a clean container.  Do not contaminate the specimen with urine or menstrual discharge.  Void first so urine is not in stool sample.  Do not place toilet tissue in the bedpan or specimen container.  Notify the nurse as soon as possible after defecation and when specimen is available.

27 # Sputum : Is the mucous secretion from the lung bronchi, and trachea # Saliva: the clear liquid secreted by the salivary glands in the mouth

28  For culture  For cytology (to identify structure and pathology of cells such as to identify cancer in the lung  For acid fast bacillus to identify the presence of TB

29 - In the morning. - During postural drainage. - Use pharyngeal suctioning when a client can not cough.

30 Sample collected from the mucosa of the oropharynx and tonsillar regions using culture swab - Puts clean gloves. - Inserts the swab into oropharynx and runs swab along the tonsils and are on the pharynx that are red or contain exudates.

31 WHO/CDS/EPR/ARO/2006.1

32  Tilt head backwards  Insert flexible fine-shafted polyester swab into nostril and back to nasopharynx  Leave in place a few seconds  Withdraw slowly; rotating motion WHO/CDS/EPR/ARO/2006.1

33 1- An aerobic specimen (Superficial Wounds ) 2- Anaerobic specimen (Deep Wounds: or abscess culture )

34  Lightly palpate wound edges to detect localized areas of drainage collection  Clean a wound with normal saline to remove skin flora.  Use sterile swab from a culturette tube

35  If wound edges are separated the nurse gently inserts the tip of the swab into the wound to collect deeper secretions.

36  After collecting the specimen the nurse returns the swab to the culturette tube, and caps the tube.  Sends the labeled specimen to the laboratory.

37  Clean site by wiping with sterile saline or 70% alcohol.  The nurse uses a sterile syringe tip to aspirate drainage from inner wound.  Afterward the nurse apply a sterile needle to the syringe.  Expels the air from syringe and needle and place a cork over needle to prevent entrance of air.

38  The client’s name.  Hospital number.  Age and gender.  Exact nature and source of the specimen.  Diagnosis.  Date and time of specimen collection.  Ordering physician (Test requested or laboratory test desired).  Antimicrobial therapy ( medication as antibiotic ).

39  Provide client comfort, privacy and safety  Explain the purpose of the specimen collection  Explain the procedure  Use the correct procedure for obtaining a specimen  Hand washing & Use aseptic technique.

40  Note relevant information on the laboratory requisition.  Make sure that the specimen label is found.  Transport the specimen to the laboratory promptly.  Report abnormal laboratory finding to health care provider.



Download ppt "Prepared by Mrs / Hamdia Mohammed. At the end of this lecture each student should be able to: 1. Define purposes of a specimen collection. 2. Explain."

Similar presentations

Ads by Google