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Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of.

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Presentation on theme: "Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of."— Presentation transcript:

1 Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes with chromosome paint Chromosome errors diagnosed by DNA- content in sperm cells

2 Preparations Growing of lymphocytes Harvesting of cells Staining Growing of other cell types

3 Normal karyotype, cattle Number of chromosomes: 2N = 60, XY

4 Normal karyotype, swine 2N = 38, XX X

5 Normal karyotype, cat 2N = 38 XY

6 Chromosome errors, cattle Trisomy 22, 2N = 61, XX Translocation 1-8, 2N = 60, XY

7 Less severe chromosome errors Known centromere fusiones Cattle 1/29 Blue fox 23/24 Swine 14/16 Female chromosome Number of litters Litter size , , ,2 A centromere fusion in heterozygotes lowers the fertility with ca. 10 % Fusion 1/29 Chromosome 1 and 29

8 Chromosome paint Identity of a chromosome or piece of chromosome diagnosed by chromosome paint Application of species specific probes: cDNA, cosmids, bac clones etc. Application of chromosome specific libraries (paints): Specie specific (Homologous) From another specie (Heterologous)

9 Less severe chromosome errors S ex-chromosome errors: XY, XXX and X0 mares Blood chimeras in cattle twins: Freemartins

10 Chromosome paint: Example with Swine Application of swine chromosome 12 p-arm from library of swine chromosomes Back ground picture: Two yellow spots are the p-arm swine chromosome 12 Black and white picture of same paint with two stained chromosome pices

11 Chromosome paint: Example with humans The human chromosome each painted in its own colour Metaphase Karyotype Black and white picture and two stained chromosomes showing a translocation

12 Chromosome paint Example using a mink cosmid

13 Example using heterologous paint Mink 13 Human 14 probe

14 Chromosome errors diagnosed by DNA-content in sperm cells Flow-cytometri on sperm cells normal boar Y X { 4% deviation

15 Chromosome errors diagnosed by DNA-content in sperm cells Translocation boar

16 Chromosome specific DNA- probe for diagnosis of non- disjunction in sperm cells Mink chromosome 8 specific probe


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