Changes in G protein expression in ethanol treated drosophila (acute effect) Relative Gene Expression Gq G 5 G 76C Control Treated 2.0 1.5 1 0.5 0.
Type of changes Acute effect (naïve flies) Acute effect (‘cronic’ flies) Cronic effect (‘cronic’ flies)
System complexity G protein coupled Receptors Dopamine R Opiate R Serotonin R GABA Glutamate Etc,etc G protein coupled Receptors Dopamine R Opiate R Serotonin R GABA Glutamate Etc,etc G proteins Alpha s i o ….. Beta 1 2 3 ….. Gamma 1 2 3 ……. G proteins Alpha s i o ….. Beta 1 2 3 ….. Gamma 1 2 3 ……. Effectors Adenylate cyclase PLC K+ channels Effectors Adenylate cyclase PLC K+ channels
Rationale for studying G proteins gene expression GTP Normal Tolerance Effect A Effect B Protein X Protein Y
System variability (are all flies equal?) Relative Gene Expression Gq G 5 G 76C Control Treated 2.0 1.5 1 0.5 0.
Onset of sedation induced by alcohol Time (sec)
Onset of sedation induced by alcohol Time (sec) Early Female Flies Late Female Flies
Onset of sedation induced by alcohol Time (sec) Early Males Flies Late Males Flies
Genetic differences (Genetic crosses) Early male Early female Late male Late female F1 Early F1 Late
Changes in gene expression on a variable genetic background Ethanol Gene Expression
Other ‘addiction’ research at UEL Substance use and misuse ‘ network’ SUM-UEL
SUM Substance Use and Misuse Substance Use and Misuse Poverty Physical Addiction Peer pressure Experimentation Crime Education (lack of) Psychological wellbeing Genetics
Cognitive Studies Clinical Interventions Ethnographic Studies Toxicology Pharmacology Training Education Social Policies UEL SUM Network UEL SUM Network Child Development
Get in touch SUM ‘network members’ Dr Daniel Briggs LSS Dr. Stefano Casalotti. HSB Dr Olivia Corcoran HSB Dr. Lynne DawkinsPsychology Prof. Angela Harden. HSB Stephanie Lynch Psychology John McCarthy. Student Services Prof. Mark McDermott Psychology Dr. Olive McKeown. HSB Prof. Derek Moore Psychology Dr Winston Morgan. HSB Dr Barbara Pendry. HSB Dr Alice Sampson LSS Dr. Kirstie Soar.Psychology Dr. John Turner Psychology Oghenetega Umukoro HSB Contact: John Turner Turner2@uel.ac.uk Stefano Casalotti firstname.lastname@example.org