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Spectroscopy of Biopolymers Introduction to Biopolymers (II) Nucleic Acids.

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Presentation on theme: "Spectroscopy of Biopolymers Introduction to Biopolymers (II) Nucleic Acids."— Presentation transcript:

1 Spectroscopy of Biopolymers Introduction to Biopolymers (II) Nucleic Acids

2 Structure of nucleic acid

3 去氧核醣核酸 DNA

4 磷酸 鹼基 五碳醣 核甘核甘 核甘酸核甘酸 鹼基間氫鍵 主 鏈 去氧核醣核酸 DNA

5 A T G C

6 Torsion Angles in nucleic Acids The structure of nucleic acids are defined by the torsion angles along the phosphoribose backbone  t   ), the torsion angles within the sugar ring(  to  ) and the rotation of the nucleobase relative to the sugar (  ). Rotation about  places the base either extended from the ribose (anti) or sitting above the ring (syn)

7 Sugar conformation of nucleic acids

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9 去氧核醣核酸 DNA

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14 A-Form RNA or DNA Most RNA and RNA-DNA duplex in this form Shorter, wider helix than B. Deep, narrow major groove not easily accessible to proteins Wide, shallow minor groove accessible to proteins, but lower information content than major groove. Favored conformation at low water concentrations Base pairs tilted to helix axis and displaced from axis Sugar pucker C3'-endo (in RNA 2'-OH inhibits C2'-endo conformation)

15 B-Form DNA Most common DNA conformation in vivo Narrower, more elongated helix than A. Wide major groove easily accessible to proteins Narrow minor groove Favored conformation at high water concentrations (hydration of minor groove seems to favor B-form) Base pairs nearly perpendicular to helix axis Sugar pucker C2'-endo Look at this spacefilling view of B-form DNA.

16 Helix has left-handed sense Can be formed in vivo, given proper sequence and superhelical tension, but function remains obscure. Narrower, more elongated helix than A or B. Major "groove" not really groove Narrow minor groove Conformation favored by high salt concentrations, some base substitutions, but requires alternating purine- pyrimidine sequence. N2-amino of G H-bonds to 5' PO: explains slow exchange of proton, need for G purine. Base pairs nearly perpendicular to helix axis

17 Z-form DNA GpC repeat, not single base-pair P-P distances: vary for GpC and CpG GpC stack: good base overlap CpG: less overlap. Zigzag backbone due to C sugar conformation compensating for G glycosidic bond conformation Conformations: G; syn, C2'-endo C; anti, C3'-endo

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