Presentation on theme: "Chair of Microbiology, Virology, and Immunology"— Presentation transcript:
1Chair of Microbiology, Virology, and Immunology Shigella.
2Causative agents of Shigellosis The causative agent of dysentery was described in 1888 by A. Chantemesse and in 1891 by A. Grigoryev and F. Widal. In 1898 this organism was studied in detail by K. Shiga in Japan and in by V. Kruse in Germany (Shiga bacillus).
3Morphology. Morphologically dysentery bacilli correspond to the organisms of the family Enterobacteriaceae. Dysentery bacilli have no flagella and this is one of the differential characters between these organisms and bacteria of the coli-typhoid-paratyphoid group. Some strains of Flexner bacilli are found to possess cilia.
7Toxin production. S. dysenteriae produce thermolabile exotoxin which displays marked tropism to the nervous system and intestinal mucous membrane. This toxin may be found in old meat broth cultures, lysates of a 24-hour-old agar culture, and in desiccated bacterial cells.
8The dysentery exotoxin causes the production of a corresponding antitoxin. The remaining types of dysentery bacilli produce no soluble toxins. They contain endotoxins, which are of a gluco-lipo-protein nature, and occur in the smooth but not in the rough variants.Thermolabile substances exerting a neurotropic effect were revealed in some S. sonnei strains. They were extracted from old cultures by treating the latter with trichloracetic acid.
9Antigens Subgroup Species and serotype Subse-rotypes Antigenic formula Type antigenGroup antigensA. Does not ferment manniteS.dysenteria1-12B. Ferments mannite as a ruleS. flexneri , 2, 3, 4, 5,6, X variant Y variant1a, 1b, 2a, 2b,3a, 3b, 4a, 4bI, II, III, IV, V, VI2, 3, 4, 6, 7, 8C. Ferments mannite as a ruleS. boydii,D. Ferments mannite, slowly lactose and saccharoseS. sonnei
10Epidemiology and Pathogenesis of Shigellosis Epidemiology and Pathogenesis of Shigellosis. Humans seem to be the only natural hosts for the shigellae, becoming infected after the ingestion of contaminated food or water. Unlike Salmonella, the shigellae remain localized in the intestinal epithelial cells, and the debilitating effects of shigellosis are mostly attributed to the loss of fluids, electrolytes, and nutrients and to the ulceration that occurs in the colon wall.It has been known for many years that Shigella dysenteriae type 1 secreted one or more exotoxins (called Shiga toxins), which would cause death when injected into experimental animals and fluid accumulation when placed in ligated segments of rabbit ileum.
13Laboratory diagnosis. Reliable results of laboratory examination depend, to a large extent, on correct sampling of stool specimens and its immediate inoculation onto a selective differential medium. The procedure should be carried out at the patient's bedside, and the plate sent to the laboratory.
14For the serological diagnosis of dysentery the indirect haemagglutination (IHA) test with erythrocyte diagnosticums with the titre of 1:160 and higher is performed. The test. is repeated after at least seven days. Diagnostically important is a four-fold rise in the antibody litre, which can be elicited from the 10th-12th day of the disease. To distinguish between patients with subclinical forms of the disease and Shigella carriers, identify immunoglobulins of the G class.
15Treatment and Control of Shigellosis Intravenous replacement of fluids and electrolytes plus antibiotic therapy are used for severe cases of shigellosis. Ampicillin frequently is not effective, and alternative therapies include sulfamethoxazole/ trimethoprim and, with increasing sulfamethoxazole/ trimethoprim resistance, the quinolone antibiotics such as nalidixic acid and ciprofloxacin.In the Far East, India, and Brazil where shigellosis is more common than in the United States, multiple antibiotic resistance because of the acquisition of plasmids has become common. Shigellosis also is common in Latin America.
16The injection of killed vaccines is worthless, because humoral IgG does not seem to be involved in immunity to the localized intestinal infection.Live vaccines that cannot grow in the absence of streptomycin (ie, streptomycin-dependent vaccines) have been developed and used in clinical trials, but success has been equivocal.
17SummarySHIGELLA1. Genus characteristicsa. Slender, gram-negative rod; non lactose-fermenting (except for S. sonnei)b. In contrast to E. coli: no H2S production, no lysine decarboxylation, no acetate utilizationc. Invasive (key to pathogenesis)d. In contrast to Salmonella: non-motile; no gas from glucose fermentation; no H2S productione
18e. Toxin production limited to a few strains f. All have O antigens-four groups (A-D)g. Differentiating speciesi. S. dysenteriae - no mannitol fermentationii. S. boydii - C antigen groupiii. S. flexneri - B antigen groupiv. S. sonnei-orniltine decarbexylase production
19h. Specimensi. Rectal swab from colonic ulcer is best for culture.ii. Fecal specimen - must be immediately innoculated onto transport media or culture media. Sensitive to acids present in feces.
202. Epidemiology a. Species i. S. sonnei most frequent isolate in U.S. ii. S. dysenteriae and S. boydii most frequently isolated in developing countries - cause more severe disease course.iii. S. flexneri more commonly isolated from homosexual men b. Humans and higher primates are only natural reservoir. c. High communicability (< 200 bacilli needed to produce disease) d. May be spread by fomites;”food, fingers, feces, and files”.
213. Clinical manifestations a. Desease - bacillary dysentery (shigellosis), characterized by painful, frequent, low-volume stools; feces contain blood and mucous. Bacteremia is rare since organisms usually do not enter bloodstream. Polymorphonuclear leukocyles present in stool. b. Carriersi. One to four weeks after the disease; carrier state may be set up if organism is not cleared. ii. Long-term; recurrent bouts of disease
224. Therapy and prevention a. Hydration and electrolyte replacement b. Ampicillin - decreases duration of symptoms and carrier state; tetracycline; trimethoprim-sulfamelhoxazole c. Prevention through personal hygiene, proper garbage disposal and water purification.
24The causative agents of cholera are the classical Vibrio cholera biovars discovered by R. Koch in 1883 and the El Tor vibrio biovar isolated from the cadaver of a pilgrim on the Sinai peninsula by Gotschlich in Vibrio cholerae biovar Proteus (N. Gamaleya, 1888) and Vibrio cholerae biovar albensis were discovered" later. V. cholerae was described by F. Pacini in 1854.
25Classification. Vibrio cholerae belongs to family Vibrionaceae, genus Vibrio consisting of 5 species. The species Vibrio cholerae is subdivided into four biological variants: biovar cholerae, biovar El Tor, biovar Proteus, and biovar albensis.Biovar cholerae and biovar El Tor of Vibrio cholerae are the causative agents of human cholera. Biovar Proteus of Vibrio cholerae causes diarrhoea in birds and gastroenteritis in humans; biovar albensis of Vibrio cholerae was revealed in fresh water and in human faeces and bile.
26Morphology. Cholera vibrios are shaped like a comma or a curved rod measuring 1-5 mcm in length and 0.3 mcm in breadthThey are very actively motile, monotrichous, nonsporeforming, noncapsulated, and Gram-negative.
29Cultivation. Cholera vibrios are facultative (anaerobes) Cultivation. Cholera vibrios are facultative (anaerobes). The optimum growth temperature is 37° C, and growth is arrested below 14 °C and above 42° C. The organisms grow readily on alkaline media at pH , and on solid media the colonies are transparent with a light-blue hue, forming domes with smooth edges.
30Fermentative properties VibrioFermentationwithin 24 hrsSheep erythrocyte hemolysisLysis by specific O-1 subgroup phagesAgglutination by O-1 cholera serumSensitivity to polymixin BsacharosemannosearabinoseVibrio cholerae biovar choleraeA–+Vibrio cholerae biovar El TorVibrio cholerae biovar ProteusVibrio cholerae biovar albensisА
31Toxin production. The cholera vibrio produces an exotoxin (cholerogen) which is marked by an enterotoxic effect and plays an important role in the pathogenesis of cholera; the endotoxin also exerts a powerful toxic effect. The cholera vibrios produce fibrinolysin, hyaluronidase, collagenase, mucinase, lecithinase, neuraminidase, and proteinases.
32Remember that non-O1 and non-O139 strains of V Remember that non-O1 and non-O139 strains of V.cholerae also cause a wide spectrum of infections, ranging from mild diarrhea to one indistinguishable from classic cholera. Some of these serotypes are known to produce a choleratoxin that is identical to that of the classic biotypes, whereas other products a heat-stable enterotoxin analogous to the ST of E. coli.
35Antigenic structure. The cholera vibrios have thermostable O-antigens (somatic) and thermolabile H-antigens (flagellar). The O-antigen possesses species and type specificity, the H-antigen is common for the genus Vibrio. The cholerae vibrios, El Tor biovars and biovars cholera belong to the O-1 subgroup.
36Pathogenesis and diseases in man Pathogenesis and diseases in man. Cholera is undoubtedly the most dramatic of the water-borne diseases. As far as is known, cholera was confined to India for the almost 2000 years between its first description by Hindu physicians in 400 b c and its spread to Arabia, Persia, Turkey, and Southern Russia in the early1800s. There were six major pandemics of cholera during the 1800s covering the entire world, killing millions wherever it struck.
38Three phases can be distinguished in the development of the disease. 1. Cholera enteritis (choleric diarrhoea) which lasts 1 or 2 days.2. Cholera gastroenteritis. Profuse diarrhoea and continuous vomiting lead to dehydration of the patient's body and this results in lowering of body temperature, decrease in the amount of urine excreted, drastic decrease in the number of mineral and protein substance, and the appearance of convulsions. The presence of cholera vibrios is revealed guite frequently in3. Cholera algid which is characterized by severe symptoms. The skin becomes wrinkled due to the loss of water, cyanosis appears, and the voice becomes husky and is sometimes lost completely. The body temperature falls to °C. As a result of blood concentration cardiac activity is drastically weakened and urination is suppressed.
40Laboratory diagnosis. A strict regimen is established in the laboratory. Examinations are carried out in accordance with the general rules observed for particularly hazardous diseases.Test specimens are collected from stools, vomit, organs obtained at autopsy, water, objects contaminated by patient's stools, and, in some cases, from foodstuffs. Certain rules are observed when the material is collected and transported to the laboratory, and examination is made in the following stages.
411. Stool smears stained by a water solution of fuchsin are examined microscopically. In the smears, the cholera vibrios occur in groups similar to shoals of fish (Fig. 3).2. A stool sample is inoculated into 1 per cent peptone water and alkaline agar. After 6 hours incubation at 37°C the cholera vibrios form a thin pellicle in the peptone water, which adheres to the glass. The pellicle smears are Gram stained, and the culture is examined for motility. A slide agglutination reaction is performed with specific agglutinating O-serum diluted in a ratio of 1 in 100.
43The organisms are then transferred from the peptone water onto alkaline agar for isolation of the pure culture. If the first generation of the vibrios in peptone water is not visible, a drop taken from the surface layer is re-inoculated into another tube of peptone water. In some cases with such re-inoculations, an increase in the number of vibrios is achieved.The vibrio culture grown on solid media is examined for motility and agglutinable properties. Then it is subcultured on an agar slant to obtain the pure culture.
443. The organism is identified finally by its agglutination reaction with specific O-serum, determination of its fermentative properties (fermentation of mannose, saccharose, and arabinose), and its susceptibility to phagolysis
45Treatment. The mortality rate of cholera can be reduced to less than1% by the adequate replacement of fluids and electrolytes. Antibiotics of the tetracycline group (tetracycline, sigmamycin), amphenicol, and streptomycin are prescribed at first intravenously and then by mouth.Pathogenetic therapy is very important: control of dehydration, hypoproteinaemia, metabolic disorders, and the consequences of toxicosis, acidosis in particular, by infusion of saline (sodium and potassium) solutions, infusion of plasma or dry serum, glucose, the use of warm bath, administration of drugs which improve the tone of the heart and vessels.
46Prophylaxis. The following measures are applied in a cholera focus: (1) detection of the first cases with cholera, careful registration of all sick individuals, immediate information of health protection organs;2) isolation and hospitalization, according to special rules, of all sick individuals and carriers, observation and laboratory testing of all contacts;(3) concurrent and final disinfection in departments for cholera patients and in the focus;
47(4) protection of sources of water supply, stricter sanitary control over catering establishments, control of flies; in view of the possibility of El Tor vibrio reproducing in water reservoirs under favourable conditions (temperature, the presence of nutrient substrates) systematic bacteriological control over water reservoirs has become necessary, especially in places of mass rest and recreation of the population in the summer;
48(5) strict observance of individual hygiene; boiling or proper chlorination of water, decontamination of dishes, hand washing;(6) specific prophylaxis: immunization with the cholera monovaccine containing 8 thousand million microbial bodies per 1 ml or with the cholera anatoxin. Chemoprophylaxis with oral tetracycline is conducted for persons who were in contact with the sick individual or for patients with suspected cholera.
49SUMMURYI. GENUS VIBRIOA. GENERAL CHARACTERISTICS1. From family Vibnonaceae 2 Naturally occur in water-marine and fresh waters; some occur in cold-blooded animals.3. Oxidase-positive-differentiates Vibrios from Enterobacteriaceae.4. Characteristic comma shape
50B VIBRIO CHOLERAE1 Species characteristicsa. Comma-shaped when first isolatedb. Aerobicc. Unipolar flagellum-motiled. Primary isolation-simple media, MacConke/s agar, tellurite taurocholate gelatin agar (TTGA),thiosulfate citrate bile salts agar (TCBS) e. Sensitive to 2,4-diamme-6,7 d isopropyi pteridine (0/129), useful in distinguishing from othergram-negative, oxidase-posilive bacilli (i.e., Aeromonas) f. Prefer alkaline environment.
512. Serogroups-based on O antigen type a. V.cholerae O-1 i. Agglutinate antisera against O-1 antigen.ii. Classic epidemic and pandemic Asiatic cholera; most virulent serogroup iii. Produce disease via enterotoxin (heat-labile toxin produciog-secretory diarrhea via cyclic AMP) iv. Subdivided biochemically into Cholerae and El Tor biotypes b. Atypical V. cholerae O-1i. Agglutinate antisera against O-1 antigen. ii. Do not cause human disease. in. Do not elaborate classic cholera enlerotoxin, ' c. Non O-1 V choleraei. Fail to agglutinate O-1 antisera.ii. Biochemically and genetically indistinguishable from the O-1 group iii. Cholera-like diarrhea; rareirr extrainistinal infection
523. Determinants of pathogenicity a. Adherence to small intestine epithelium - non-invasive infection b. Pathogenicily related to host response to (he enterotoxin (choleragen); humans only host that has pathogenic responsec. Two major enterotoxin subunitsi. A subunit-A1 peptide promotes activation of adenylate cyclose ii. subunit - binds toxin to small intestinal ganglioside receptord. Principal effect is increased intracettular cyclic AMP resulting in electrolyte/fluid secretion into small intestine lumen.
534. Clinical manifestations: Cholera a Incubation of 1-4 days b Severe, watery ("rice water") diarrhea with loss of sodium, chloride, potassium, and bicarbonatec Associated with nausea, vomiting and abdominal cramps d Metabolic acidosis e. Hypovolemic shock f. Mortality rate without treatment 25-50%
545. Therapy and prevention a. Rapid rehydration and electrolyte replacementi. Intravenous in severe cases ii. Oral therapy in milder cases b. Tetracycine (oral)i. Reduces number of organisms. ii. Decreases degree of stool output iii. Helps eliminate chronic carrier slate iv. Resistant forms recognized in Africa-plasmid mediated v. Not indicated for prophylaxis c. Present vaccine does not afford adequate protection d. Gastric acidity affords some protection. e. Infection does provide non-lasting immunity to reinfection
55C. VIBRIO PARAHEMOLYTICUS 1. Species characteristicsa. Resembles V. cholerae structurally.b. Halophilic, requiring at least 2 % NaCl for growth (in contrast to V.cholerae which does not grow in saline)c O and K antigens useful for serologic typingd. Isolates causing diarrhea produce hemolysm (Kanawaga positive)
562. Clinical manifestations a. Most cases of diarrhea attributed to mgestion of raw or improperly handled seafoodb. Incubation period hoursc. Explosive watery diairhea, headache, abdominal cramps, fever, and vomiting. Note: Diarrhea may be bloody.d. Cloudy swelling, fatty infiltration of livere. Septicemia (particularly with underlying liver disease)Localized wound infections from contamination of sea water
573. Therapy and prevention a. Mild disease usually self-limiting; no therapy required. Usually subsides within 2-4 daysb. For severe case, fluid and electrolyte replacements, antibioticsc. Organisms usually sensitive to chloramphemcol, tetracychne, and cephalosporinsd. Adequate refrigeration of raw and cooked seafood aid in prevention
58D OTHER VIBRIO INFECTIONS 1. V. alginotyticusa. Affects wounds, eyes and ears in person with injuries or seawater contact.b. Septicemia - unusualc. No known intestinal disease2. V. vulnificusa. Wound infection with intense pustules or bullaeb. Septicemjac. Occasionally produce enteritis.3. V.fluvialis,V.hollisaea. Severe diarrhea in children in Bangladeshb. Abdominal pain, lever, bloody mucus in stool 4 V.mimicus - diarrneal illness from uncooked seafood, especially oysters
59OTHER GENERA FROM FAMILY VIBRIONACEAE A. GENUS AEROMONAS1. Genus characteristicsa. Free-living, gram-negative rods located in fresh water and rarely marine lifeb. A. hydrophila is most important speciesc. Oxidase-posilive (differentiates from gram-negative enteric's). Produce large zone of hemolysis on blood agar. d Single polar flagellum - motilee. Resistance to compound O129 and lack of growth in NaCI solution differentiates from Vibrios 2 Clinical manifestationsa Causes diarrhea, septicemia, osteomyelills, and wound infection, usually in immunocompromised patientsb. Isolated from patients with and without diarrheal disease 3. Therapy - usually sensitive to aminoglycosides, tetracycline, and cephalosporins
60B GENUS PlESIOMONAS1 Genus characteristicsa. A gram-negative rod with polar flagella found in tropics and sub-tropicsb. Oxidase-positive - important to differentiate from Shigella as they share common antigensc. Has arginine, ornithine, and lysine decarboxylase - distinguishes from Aeromonas.2. Clinical manifestationsa. P. shigelloides primarily causes gastroenteritis b. Has been isolated from blood and spinal fluid
61CAMPYLOBACTER A CENUS CHARACTERISTICS 1 Isolation of comma-shaped motile gram-negative organisms from rectal or stool specimens cultured selective media containing various antibiotics that inhibit other fecal flora a. Campy-BAP-vanco, polymixin, trimethoprim and cephalothinb. Skirrow's medium-vancomycin, polymixin B and trimethoprimc. Butzler's medium2. Microaerophilic - grows best in 5 % O2 (compared to 20% atmospherically) present.3. Do not oxidize or ferment carbohydrates.4. Oxidase and catalase positive5. Reservoir: domestic animals such as dogs, cows, and chicken6. Fecal-oral transmission
62B C. JEJUNISpecies characteristicsa. Grows at 39 C and 42C with candle jar, 42 will inhibit growth of other bacteria that are present (including C. intestinalis) b. Nalidixic acid sensitive (in contrast to C.intestimalis)2. Clinical manifestations: Enterocolitisa. Invasive enteritis with bloody diarrhea, crampy abdominal pains, malaise and fever – usually limited to 1-week period; more common in children b. Inflammatory proctitis in homosexuals c. Reactive arthritis may follow in individuals HLA-B27(+).3. Thefapy - erythromycin
63C. C. INTESTINALIS (C. FETUS SUBSP. FETUS) 1. Species characteristicsa. Grows best at 25 C and 37 C and poorly at 42 C (in contrast to C.jejuni) b. Nalidixic asid resistant2. Clinical manifestationa. Bacteremiab. Opportunistic pathogen in debilitated patients c. May localize to peritoneum, pleura, lung, pericardium, j joints, or meninges. d. May cause localized diarrheal illness.3. Therapy - aminoglycoside
64C. HELICOBACTER PYLORI (previously C. pylori) 1. Species characteristics a. Spiral-shaped, motile rodb. Produces ureasec. Present in gastric mucosa of fewer than 20% of people less than 30 years old but increases to greater than 50% of people over 60 years old, even in asymptomatic patientsd. Optimal growth at pH ; organism is sheltered from lumenal acidity by embedding into gastric mucosa.
652 Clinical manifestations a. Associated with antral gastritis and peptic ulcer disease b. No direct evidence that organism causes disease but strong association existsc. Eradication of H. pylori will help heal peptic ulcer and improvement of gastritis. 3. Diagnosisa. Best made histologically via biopsy with ulceration and gastritis seen with Giemsa stain; characteristic intra-epithelial curved organismsb Urease test is used for presumptive presence of H. pylori 4. Therapy - long term (1 month) therapy with bismuth (Pepto Bismol), ampicillin, and metronidazole