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Lab 6 THE RESPIRATORY SYSTEM & SPIROMETRY. LAB 6 ACTIVITIES Identify parts of the respiratory system Respiratory tree, thoracic organs, and larynx models.

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Presentation on theme: "Lab 6 THE RESPIRATORY SYSTEM & SPIROMETRY. LAB 6 ACTIVITIES Identify parts of the respiratory system Respiratory tree, thoracic organs, and larynx models."— Presentation transcript:

1 Lab 6 THE RESPIRATORY SYSTEM & SPIROMETRY

2 LAB 6 ACTIVITIES Identify parts of the respiratory system Respiratory tree, thoracic organs, and larynx models Fresh lung (if available) Examine histology of the: Trachea Lung Examine the operation of the lung model Biopac L012-Pulmonary Function I Measure lung volumes and capacities

3 RESPIRATORY SYSTEM STRUCTURE 1.Conduction zone: pathway for pulmonary ventilation 2.Respiratory zone: membrane for gas exchange  external respiration Clinically, two parts: Upper respiratory tract Lower respiratory tract

4 RESPIRATORY SYSTEM STRUCTURE Histology reflects the different functions of the different parts of the system Position in respiratory pathway determines cell type non-keratinized stratified squamous pseudostratified ciliated columnar cuboidal ciliated simple squamous / Type I Alveolar cells simple squamous / Type I Alveolar cells

5

6 PSEUDOSTRATIFIED COLUMNAR EPITHELIUM Lamina Propria Hyaline Cartilage mucosa Submucosa

7 seudostratified epithelium = lamina propria ucous glands mooth muscle artilage

8 GROSS SECTION THROUGH LUNG

9 Smoker’s Lungs Non-smoker 

10 SMALL BRONCHIOLE

11 LUNG TISSUE SLIDE Respiratory Bronchiole Alveolar Duct Alveoli Alveolar Sac

12 CAPILLARIES IN ALVEOLAR WALL

13 SPIROMETRY

14 Total Lung Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) + Residual Volume (RV) Vital Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) Inspiratory Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) Functional Residual Capacity = Expiratory Reserve Volume (ERV) + Residual Volume (RV) SPIROMETRY – RESPIRATORY VOLUMES AND CAPACITIES

15 SPIROMETRY: CALIBRATION Insert the calibration syringe/filter assembly into the side of the airflow transducer labeled "Inlet." Inlet Follow pop up window instructions Wait for 8 seconds of no air flow, Then do your 5 syringe cycles. Inlet

16 Experimental Treatments- Different from Biopac!!!! and don’t forget the nose clip!!! 1.4 normal breaths (4 inhales/4 exhales) 2.Inhale as deeply as possible 3.Exhale to normal depth 4.3 normal breaths 5.Exhale as much as possible at end of 3 rd exhalation 6.3 normal breaths 7.Inhale as much as possible, then exhale as fast and completely as possible (you will use this data segment in activity 3) 8.3 normal breaths 9.Click Stop- look at your recording and redo if necessary 10.If it’s good, select done and analyze your data.

17 BIOPAC SPIROGRAM EXAMPLE DATA 3 regular breaths 3 regular breaths 3 regular breaths 1 strongest inhale 1 regular exhale 1 strongest inhale 1 strongest exhale This part is in addition to what Biopac Lesson 12-Lung 1 prompts you to do! If you get a negative value, just put the absolute value.

18 BIOPAC: DATA ANALYSIS Ch 2 P-P Measures Tidal Volume Record in Table 1 on page Lab 6-17

19 BIOPAC: DATA ANALYSIS P-P Measures Inspiratory Capacity Record in Table 1 on page Lab 6-17

20 BIOPAC: DATA ANALYSIS Delta Measures Inspiratory Reserve Volume Record in Table 1 on page Lab 6-17 Use similar methods to determine: Expiratory Reserve Volume Vital Capacity

21 BIOPAC: DATA ANALYSIS (ACTIVITY 5) Forced Expiratory Volume is the % of your forced vital capacity you can expel in 1,2, or 3 seconds. RESET MEASUREMENT BOXES!!!!! BOX 1- DELTA T BOX 2 P-P, ALL OTHER BOXES- NONE USE I-beam to bracket one second of data starting at peak of last maximal inhalation. P-P will give you FEV 1 then highlight the 1 st 2 seconds of data starting at peak for FEV 2 and then the 1 st 3 seconds for FEV 3 Record in Table 1 on page Lab 6-17

22 BIOPAC: DATA ANALYSIS To measure FVC highlight the recording from just before last maximal inhalation to just after peak of the last maximal exhalation- use P-P value. Record in Table 1 on page Lab 6-19

23 SUMMARY OF CLASS DATA Record your data in Excel at the computer located at the front podium Your Instructor will make the complete data set available for you to use while completing the homework assignment

24 HOMEWORK Complete and turn in the questions on pages 6-17 to 6-20 Don’t forget to print the entire spirogram (landscape format) you recorded to include in your lab report. Use vertical brackets ( [ ] ) to identify and label the tidal, inspiratory reserve, expiratory reserve and residual volumes; and total lung, vital, expiratory and inspiratory capacities on your spirogram. Lab 6-19, Question 4. Remember to completely label your graphs! Complete Assignment 6 on MasteringAandP.

25 Lab 6 THE END

26 Biopac Spirometry Residual volume

27 SPIROMETRY


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