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IRON CONTENT ANALYSIS Due Today: 1. Iron Content Analysis Pre- Lab 2. Neutralizing Power of Antacids Formal Lab Report Homework: 1. Iron Content Analysis Short Report 2. Prepare for Laboratory Final

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Introduction to ultraviolet-visible (UV-Vis) spectroscopy Introduction to analytical chemistry Determine the iron content in vitamin tablets OBJECTIVE

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KEY TERMS Visible Range: nm UV Range: nm

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Spectroscopy – measuring the interaction of radiation with matter Unique wavelength for each substance Scanning – determine wavelength with maximum absorption Chemists are most interested in the electromagnetic spectrum between nm KEY TERMS

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Use Volumetric Glassware Volumetric flask Graduated pipets Burets Use stock solutions dispensed from burets PROCEDURE NOTES

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Wipe off the cuvette with a Kim-Wipe before inserting into the Spectrophotometer Record the absorbances! Record the advertised Iron content from both vitamin bottles! PROCEDURE NOTES

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Solutions can be disposed of down the drain WASTE

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Hydrochloric Acid Danger Inhalation Hazard Corrosive RISK ASSESSMENT

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Hydroquinone Danger Harmful if Swallowed Skin Irritant RISK ASSESSMENT

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o-phenanthroline Danger Toxic if swallowed RISK ASSESSMENT

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CALCULATIONS

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Graphing in Excel Force the trendline through (0,0) Include the equation of the tendline and R 2 value CALCULATIONS Y = mx A = bc

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