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Tfam 1 g empty vector 1 g Tfb2m 1 g Relative mRNA level 0 100 200 50 150 * * p<0.05 vs empty vector * Transfection Tfam mRNA Tfb2m mRNA Tfam and Tfb2m mRNA Tfam siRNA #1 Scramble siRNA Tfb2m siRNA #1 0 0.75 0.5 0.25 1 * * Tfam siRNA #2 Tfb2m siRNA #2 * * p<0.05 vs scramble siRNA * Tfam mRNA Tfb2m mRNA Transfection Tfam and Tfb2m mRNA Tfam Tfb2m 28S Non-treated Tfam siRNA #1 Scramble siRNA Tfb2m siRNA #1 Tfam siRNA #2 Tfb2m siRNA #2 Relative mRNA level Supplemental Figure 2 B C A Transfection
A Supplemental Figure 3 Translational diffusion time ( s) 0 200 400 600 800 1000 1200 1400 -1769-1739-1709-1679-1649-1619-1589-1559-1529-1499-1469-1439-1409 p<0.05 * -1799-1829 Recombinant TFAM protein (-) (+) -1379 * B Translational diffusion time ( s) 0 200 400 600 800 1000 1200 1400 -1769-1739-1709-1679-1649-1619-1589-1559-1529-1499-1469-1439-1409 *p<0.05 * -1799-1829 Recombinant TFB2M protein (-) (+) -1379 * 1600 1800 * *
Supplemental Figure 4 A B Tfam siRNA #1 Scramble siRNA 0 15 10 5 20 * Tfb2m siRNA #1 * p<0.05 vs. scramble siRNA * ATP content (nmol/mg tissue) 0 30 20 10 40 * * p<0.05 vs. empty vector * ATP content (nmol/mg tissue) Tfam 1 g Empty vector 1 g Tfb2m 1 g
Supplemental Figure 5 AB Relative mitochondrial ATPase 6/8 mRNA level r = 0.49 p < 0.01 0.25 0.5 0.75 1.0 1.25 2.02.51.51.00.5 Relative Tfam mRNA level 1.5 1.75 2.0 Relative mitochondrial ATPase 6/8 mRNA level r = 0.57 p < 0.001 0.25 0.5 0.75 1.0 1.25 2.01.51.00.5 Relative Tfb2m mRNA level 1.5 1.75 2.0 D Relative cytochrome-b mRNA level r = 0.48 p < 0.01 0.25 0.5 0.75 1.0 1.25 2.02.51.51.00.5 Relative Tfam mRNA level 1.5 C Relative cytochrome-b mRNA level r = 0.58 p < 0.001 0.25 0.5 0.75 1.0 1.25 2.01.51.00.5 Relative Tfb2m mRNA level 1.5
Translational diffusion time ( s) -170 ~-141 -164 ~-135 -158 ~-129 -152 ~-123 -146 ~-117 -140 ~-111 -134 ~-105 -131 ~-102 -128 ~-99 -125 ~-96 -122 ~-93 ( - ) (+)(+) p<0.05 0 500 1000 1500 2000 2500 3000 * * * * * Recombinant SP1 protein A CD Tfam 0.5 g + Sp1 0.5 g 1 2 0.5 1.5 2.5 0 Empty vector 1 g Tfam 1 g Sp1 1 g 3 Serca2 gene transcription (relative luciferase unit) * * * p<0.05 vs. empty vector 0 200 400 600 800 1000 1200 TFAM only TFAM+SP1 Translational diffusion time ( s) NS Supplemental Figure 6 B Serca2 gene transcription (relative luciferase unit) p<0.05 vs. empty vector in each group * * * Serca2 Luc wild Serca2 Luc mutation -122 Serca2 Luc mutation -119 Empty Tfam Tfb2m Sp1 Empty Tfam Tfb2m Sp1 Empty Tfam Tfb2m Sp1 Empty Tfam Tfb2m Sp1 Serca2 Luc mutation -116 * * * 0 1 2 3 4 * * * *
Tfam Tfb2m Bnp Serca2a ET-1 100 nmol/L NE 30 mol/L NE 100 mol/L Vehicle 28S Supplemental Figure 7 ET-1 10 nmol/L Vehicle NE 5 mol/L Vehicle
Gene expression (fold-increase vs. untreated control) > 7-fold Insulin/Glucose axis-unrelated genes Insulin/Glucose axis-related genes a Supplemental Figure.
Knight et al – Supplementary Table Supplemental Table 1: Cell line Densitometry AROSSIRT1 ARPE WI MCF10A HCT HCT116.
Online table I: Sequences of primers used. ControlIron RM M2 I ControlIron RM M2 ML Iron *** Iron content (nmol/mg protein) **
Supplemental Table 1 Source and authentication of cell lines.
Supplemental Figure 1 A No. at risk T T T
Supplemental figure 1: Correlation coefficients between signal intensities from biological replicates of wild.
Adipose PancreasAorta MuscleHeart Liver LungKidney Testis Spleen Brian 18S p55 γ p55 α Figure S1 Tissue distribution of p55 . (A) Representative RT-PCR.
Supplemental Figure 1 a % pulldown from input LNCaP/scrambled-siRNA Figure 1 A, ChIP analysis of AR and PHB binding to the PSA promoter in the LNCaP/scrambled-siRNA.
WT KO A B Suppl. Fig 1 Supplemental Figure 1. The expression of CYLD in liver. (A) Immunohistochemical analysis of CYLD in liver tissue of adult wildtype.
SC.912.L.16.3 DNA Replication. – During DNA replication, a double-stranded DNA molecule divides into two single strands. New nucleotides bond to each.
Transcription and Translation: What does a cell (or organism) do with its genes??
Figure S1 Figure S1. (A) Screen of USPs for regulating c-Myc stability. Scores of c-Myc protein abundence was derived from two independent experiments.
Topic 2 Videos DNA Structure & Function Transcription & Translation Mutations.
Control siRNA BRG1 siRNA PTEN – Akt – p-Akt – p-GSK-3 – Cyclin D1 – BRG1 – -actin – SW480 Supplemental data S1 Supplemental data S1. Effects of transduction.
Flag-c-Myc HA-REG Flag-c-Myc WT T58/S62A HA-REG EGFP C Fig. S1 A B Supplementary Figure S1. Measure the degradation of c-Myc by REG . (A)
Marker (%) Normal EPCs Mean ± SM (%) MMD EPCs Mean ± SM (%) CD ± ± 6.8 KDR27.3 ± ± 8.90 CD ± ± 3.93 CD ±
Wu et al., Supplementary Fig 1 Supplementary Figure 1: The depletion of BRG1 in HeLa cells has no effect on cell proliferation. Sh- empty vector was used.
Project: ATP13A1-5 Tine Holemans 14/08/2009.
RNA & Protein Synthesis Chapter 13. DNA A book of instructions that tells each individual what proteins to make for their needs. The path from genes to.
Supplemental figure 1 Adipocyte SVF E CD AB Supplemental figure 1: Impaired expression of Am2 and Ramps in obese mice (A-E) mRNA levels of Am2, Crlr, Ramp1,
ER HSP90 DMSO 200 M I3C30 M DIM200 M TRYPTOPHOL Supplemental Figure 1. DIM and Tryptophol fail to induce the downregulation of ER seen with I3C.
Sh-luc shSCP1-1 * IB: c-Myc IB: Tubulin shSCP1-2 IB: p-Ser KD 70KD 60KD 70KD (abnova) sh-luc shSCP1-1 * IB: c-Myc IB: Tubulin shSCP1-2.
Analyzing the effects of a gene-specific siRNA on IL13R-α2 mRNA and protein levels in glioblastoma multiforme cells INTRODUCTION RNA interference (RNAi)
RNA carries DNA’s instructions. The central dogma states that information flows in one direction from DNA to RNA to proteins. The central dogma includes.
CELL ACTIVITIES created by Mrs. Jackson 9/2008. Cell Activities Include: Cell Transport Cell Transport Protein Synthesis Protein Synthesis Division Division.
Gene function analyses, Reporter genes in direct and reverse genetics.
Homework #2 is due 10/17 Bonus #1 is due 10/24 Office hours: M 10/ :30am 2-5pm in Bio 6.
NUTRITION AND GENE EXPRESSION February 19, 2016 PROMOTER MUTATIONS Mutations in the REGULATORY part of the gene (usually called the PROMOTER) show us the.
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CHAPTER 17 Recombinant DNA and Biotechnology
Figure 1 HuR Tis11b HPRT VEGF Time (h) A 462 bp (VEGF 165 ) 332 bp (VEGF 121 ) 354 bp 111 bp 305 bp.
Supplemental Figure S2. Location and H 2 O 2 -dependent binding of RBPs to biotinylated MKP-1 transcripts. (A) Schematic of the MKP-1 mRNA showing the.
CHAPTER 12: GENETICS.
A B C A C D E F A Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals EXT-1 tumor suppressor as a Notch1 and FBW7.
In the name of God. Summer School Influenza Unit, Pasteur Institute of Iran summer 2013 Summer School.
Relationship between Genotype and Phenotype
DNA to Protein. Chromosomes are made of tightly packed DNA A gene is a section of the DNA molecule that codes for a particular protein. The order of nitrogen.
Con WTTM Supplementary Figure 1 Supplementary Figure 1. Tumor histopathology of the lungs. (a) Histological examination of lungs of 52-week-old K- ras.
CD68 NE-SSE NE-NS Figure E1. Nrf2 42-kD actin 32-kD HO kD Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 Control.
Molecular Genetics DNA RNA Protein Phenotype Genome Gene
Date of download: 5/28/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Association of a MicroRNA/TP53 Feedback Circuitry.
Please turn in the Unknown Solutions Lab Remember: We will vote on T-shirt designs on Monday.
Fig.S1. Effects of various inhibitors on LPS-induced TNFα mRNA expression in RAW264.7 cells. The same experimental procedures were performed as described.
For transcription to begin the gene needs to be stimulated by transciptional factors. These move from the cytoplasm into the nucleus. Each transcriptional.
Exercise Induced Mitochondrial Biogenesis. What is it? Mitochondrial content is increased in the muscle due to the stress of physical activity –Greater.
CHAPTER 31 Genetic Engineering and Biotechnology.
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P247. Figure 9-1 p248 Figure 9-2 p251 p251 Figure 9-3 p253.
Supplementary Figures NormalCRC CRC+5FU CRC+CPT11 100µm S1.
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