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Adolfo Quiles and Ricardo Goenaga USDA-ARS Tropical Agriculture Research Station (TARS) Mayagüez, Puerto Rico WEBSITE: http//:www.ars-grin.gov/may Seed.

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Presentation on theme: "Adolfo Quiles and Ricardo Goenaga USDA-ARS Tropical Agriculture Research Station (TARS) Mayagüez, Puerto Rico WEBSITE: http//:www.ars-grin.gov/may Seed."— Presentation transcript:

1 Adolfo Quiles and Ricardo Goenaga USDA-ARS Tropical Agriculture Research Station (TARS) Mayagüez, Puerto Rico WEBSITE: http//:www.ars-grin.gov/may Seed Increase and Phytosanitary Assessment of Quarantined and Tropically- Adapted Genetic Resources

2 Tropical Agriculture Research Station (TARS) MISSION ASSESS TROPICAL SORGHUM AND DRY BEAN GENETIC RESOURCES FOR DISEASE RESISTANCE AND GENETIC DIVERSITY AND DEVELOP GERMPLASM ADAPTED TO TEMPERATE REGIONS. INTRODUCE, PRESERVE, EVALUATE, REGENERATE, DISTRIBUTE TROPICAL FRUIT GERMPLASM AND DEVELOP CULTURAL AND MANAGEMENT SYSTEMS FOR TROPICAL/SUBTROPICAL CROPS.

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4 TROPICAL AGRICULTURE RESEARCH STATION (TARS) FOUNDED IN BECAME PART OF THE AGRICULTURAL RESEARCH SERVICE (1961) PART OF THE U.S. NATIONAL PLANT GERMPLASM SYSTEM (NPGS) MIAMI, FL km Regeneration sites

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6 CRIS Project: D Title: Seed Increase and Phytosanitary Assessment of Quarantined and Tropically- Adapted Genetic Resources Overall Goal of the Project: To move Zea and Sorghum accessions through quarantine and to increase seed of selected genera of photoperiod sensitive crops thereby increasing genetic diversity within the collections. Objectives: 1)Increase seed and conduct phytosanitary assessments for more than 1,000 NPGS Sorghum and Zea accessions which are subject to quarantine restrictions, so as to make additional certified, pathogen-tested seeds available to users. 2)Regenerate approximately 8,000 NPGS accessions with few seeds, low viabilities, and adaptations to equatorial latitudes and long-seasons, emphasizing Sorghum, Zea, Vigna, Cucurbita, Leucaena, and Psophocarpus. Record key agronomic traits such as host-plant resistance to biotic and abiotic stresses. -- Work conducted in very close collaboration with Dr. John Erpelding, Sorghum Curator; Mr. Mark Millard, Corn Curator, Dr. Gary Pederson, Acting Cowpea Curator, Dr. Bob Jarret, Cucurbits Curator and Dr. Brad Morris, Special-purpose Legumes Curator.

7 ROLE OF CURATORS FOR SEED REGENERATION SET PRIORITIES FOR GERMPLASM TO BE GROWN. REQUEST SEED FROM NATIONAL CENTER FOR GENETIC RESOURCES PRESERVATION (NCGRP), NORTH CENTRAL REGIONAL PI STATION (NCRPIS) OR PLANT GENETIC RESOURCES CONSERVATION UNIT(PGRCU). NCGRP, NCRPIS OR PGRCU PERSONNEL SEND SEED TO ST. CROIX OR TO MAYAGUEZ. HARVESTED SEED RETURNED TO PI STATIONS.

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9 AGROECOLOGICAL CHARACTERISTICS OF ST. CROIX ISLAND IS 22 MILES LONG AND 6 MILES WIDE SOIL: CLAYEY, CALCAREOUS SEDIMENTS OVER SOFT LIMESTONE OR MARL WITH pH OF 8.0 OR HIGHER AVERAGE ANNUAL RAINFALL: 112 CENTIMETERS (44 INCHES) MONTHLY MAXIMUM TEMPERATURE: 27 TO 30 °C MONTHLY MINIMUM TEMPERATURE: 22 TO 25 °C

10 APHIS QUARANTINE PROTOCOL ( REQUIREMENTS OF APHIS DEPARTMENTAL PERMIT #60668) PREPLANTING SEED RECEIVED FROM FT. COLLINS, TREATED WITH 1.58% Na0Cl. ANNUAL DISEASE SURVEY CONDUCTED BY ARS AND APHIS PATHOLOGISTS. GROWING SEASON APHIS AND ARS PATHOLOGISTS CONDUCT INSPECTION FOR PROHIBITED DISEASES.

11 REQUIREMENTS OF APHIS DEPARTMENTAL PERMIT #60668 (CONT.) HARVEST APHIS AND ARS PATHOLOGISTS CONDUCT INSPECTION BEFORE HARVESTING OPERATION BEGIN. ARS PERSONNEL HARVEST SEED UPON APPROVAL OF PATHOLOGISTS. SEED IS SENT TO REGIONAL PLANT INTRODUCTION STATIONS. PLANT RESIDUES ARE DISPOSED OF BY DEEP PLOWING. PLOT REMAIN UNUSED FOR THREE YEARS.

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13 YEARLY GROWOUTS Sorghum Plantings: September – January (St. Croix) Corn Planting: May (St. Croix) Cowpea Planting: September (Isabela) Leucaena: September (St. Croix) Winged bean: September (St. Croix) Cucurbits: September (Isabela)

14 SORGHUM DESCRIPTORS Vigor Sprouting Tillering Midrib color Anthesis Height F. L. height B.o.P. height Pan. exsertion Midrib color Juicy/dry midrib Pan. length P. erectness Height unif. Plant color St. waxiness Rust incidence Anth. incidence Yield potential Desirability

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21 Sorghum Seed Cleaning Operation

22 CORN DESCRIPTORS Ear height Height to peduncle Total plant height Tassel type Ears/stalk Tillers/stalk Nodes/plant Nodes above ear Shank length

23 Zea Crossing and Management

24 COWPEA DESCRIPTORS FloweringFlower colorLeaf shape MaturityPlant habitPlant height Dry pod colorPod lengthPod place Pod positionPods/peduncleSeed coat color Seed patternSeed productionSeed shape Seed sizeSeed textureHarvest date Seed patt. color

25 COWPEA SEED REGENERATION

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27 CUCURBITS DESCRIPTORS Fruit color Fruit diameter Fruit length Fruit shape Fruit spotting Fruit surface Stripe color Sd. cavity color Unif. of color Unif. of F. shape Unif. of F. size Unif. of F. surface Maturity Fruit set Fruit weight Longi. grooves

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29 CUCURBIT SEED SHAPE PI PI

30 Leucaena DESCRIPTORS Branching Foliage Plant height Maturity Width Harvest date

31 Leucaena Regeneration

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34 DESCRIPTORS: OTHER CROPS Gourds: 10 descriptors Luffa: 4 descriptors Wingbean: 2 descriptors

35 Winged Bean Regeneration

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37 Sorghum seedstock production at the USDA-ARS, Germplasm Introduction Research Unit Quarantine Increase Non-quarantine Increase Year Planted Harvested Planted Harvested Distribution PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU pending 1456 pending PGRCU TOTAL Grand Total

38 Corn seedstock production at the USDA-ARS, Germplasm Introduction Research Unit Quarantine Increase Non-quarantine Increase Year Planted Harvested Planted Harvested Destination NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS NCRPIS TOTAL GRAND TOTAL

39 Cowpea seedstock (non-quarantined) production at the Germplasm Introduction and Research Unit and Isabela Research FarmAccessions Year Planted Harvested Destination PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU TOTAL

40 Leucaena seedstock production at the USDA-ARS, Germplasm Introduction and Research Unit AccessionsAccessions Year Planted Harvested Destination PGRCU PGRCU PGRCU PGRCU PGRCU Pending PGRCU TOTAL 85 75

41 Cucurbit seedstock production at the USDA-ARS, Research Isabela Farm AccessionsAccessions Year Planted Harvested Destination PGRCU PGRCU PGRCU PGRCU PGRCU PGRCU Pending PGRCU TOTAL 65 28

42 Winged bean seedstock production at the USDA- ARS, Germplasm Introduction and Research Unit Accessions Year Planted Harvested Destination PGRCU PGRCU PGRCU PGRCU PGRCU Pending PGRCU TOTAL 57 39

43 Pearl Millet seedstock production at the Germplasm Introduction and Research Unit Accessions Year Planted Harvested Destination PGRCU PGRCU PGRCU PGRCU PGRCU TOTAL

44 THANKS, QUESTIONS?


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