1. The Bacteria Phylogenetic tree of the major lineages of Bacteria based on 16S ribosomal RNA Sequence comparisons

2. The Purple Bacteria, also called Proteobacteria is the largest and most physiological diverse of all bacteria

3. Bacteria §Purple and Green (Anoxygenic Phototrophic Bacteria)Purple and Green (Anoxygenic Phototrophic Bacteria) §CyanobacteriaCyanobacteria §ProchlorophytesProchlorophytes §Chemolithotrophs: Nitrifying BacteriaChemolithotrophs: Nitrifying Bacteria §Chemolithotrophs: Sulfur- and Iron-Oxidizing BacteriaChemolithotrophs: Sulfur- and Iron-Oxidizing Bacteria §Chemolithotrophs: Hydrogen-Oxidizing BacteriaChemolithotrophs: Hydrogen-Oxidizing Bacteria §Methanotrophs and MethylotrophsMethanotrophs and Methylotrophs §Sulfate and Sulfur-Reducing BacteriaSulfate and Sulfur-Reducing Bacteria §Homoacetogenic BacteriaHomoacetogenic Bacteria §Budding and Appendaged (Prosthecate) BacteriaBudding and Appendaged (Prosthecate) Bacteria §SpirillaSpirilla §SpirochetesSpirochetes §Gliding BacteriaGliding Bacteria §Sheathed BacteriaSheathed Bacteria

4. Bacteria §PseudomonadsPseudomonads §Free-Living Aerobic Nitrogen-Fixing BacteriaFree-Living Aerobic Nitrogen-Fixing Bacteria §Acetic Acid BacteriaAcetic Acid Bacteria §Zymomonas and ChromobacteriumZymomonas and Chromobacterium §Vibrio and Related GeneraVibrio and Related Genera §Facultatively Aerobic Gram-Negative RodsFacultatively Aerobic Gram-Negative Rods §Neisseria and other Gram-Negative CocciNeisseria and other Gram-Negative Cocci §RickettsiasRickettsias §ClamydiasClamydias §Gram-Positive Bacteria: CocciGram-Positive Bacteria: Cocci §Lactic Acid BacteriaLactic Acid Bacteria §Endospore-Forming Gram-Positive Rods and CocciEndospore-Forming Gram-Positive Rods and Cocci §MycoplasmasMycoplasmas §High GC Gram-Positive Bacteria: “Actinomycetes”High GC Gram-Positive Bacteria: “Actinomycetes” §Coryneform BacteriaCoryneform Bacteria §Propionic Acid BacteriaPropionic Acid Bacteria §MycobacteriaMycobacteria §Filamentoud ActinomycetesFilamentoud Actinomycetes

5. Sulfate- and Sulfur-Reducing Bacteria Representative Sulfate-reducing and sulfur-reducing bacteria Most are Delta Purple Bacteria Desulfovibrio desulfuricans Desulfonema limicola Desulfobacter postgatei Desulfobulbus propionicus Desulfosarcina variabilis Desulfuromonas acetoxidans Sulfur-reducing bacteria

6. Sulfate-Reducing Bacteria §Strict anaerobes, §Divided into two broad physiological subgroups: l Genera in Group I (Desulfovibrio, Desulfomonas, Desulfotomaculum, Desulfobulbus) utilize lactate, pyruvate, ethanol, or certain fatty acids as carbon and energy sources, reducing sulfate to hydrogen sulfide l Genera in Group II (Desulfococcus, Desulfosarcina, Desulfonema, Desulfonema) specialize in the oxidation of fatty acids, particularly acetate, reducing sulfate to sulfide §Growth and reduction of sulfate by Desulfotomaculum in certain canned foods leads to a type of spoilage called sulfide stinker, §Habitants of anoxic aquatic and terrestrial environments

7. Sulfur-Reducing Bacteria §Able to reduce elemental sulfur to sulfide §Unable to reduce sulfate to sulfide §Obligate anaerobes §Utilize only sulfur as an electron acceptor §Also referred to as dissimilatory sulfur- reducing bacteria §Members of the genus Desulfuromonas can grow anaerobically by coupling the oxidation of substrates such as acetate to ethanol to the reduction of elemental sulfur to hydrogen sulfide

8. Homoacetogenic Bacteria §Obligate anaerobes §Gram-positive §CO 2 as a terminal electron acceptor §Acetate as the sole product of anaerobic respiration §Acetyl-CoA pathway convert CO 2 to acetate §Typical species: Acetobacterium woodii and Clostridium aceticum

9. Homoacetogenic Bacteria Mechanism of autotrophy in homoacetogenic, sulfate-reducing and methanogenic bacteria Reactions of the Acetyl-CoA Pathway

10. Budding and Appendaged (Prosthecate) Bacteria Asticcacaulis biprosthecum Ancalomicrobium adetum Ancalochloris perfilievii Stella

11. Budding and Appendaged (Prosthecate) Bacteria §Contain various kinds of cytoplasmic extrusions: l Stalk l Hyphae l Appendages §These kinds of extrusions, which are smaller in diameter than mature cell, contain cytoplasma, and are bounded by the cell wall, are called prosthecae (singular prostheca) §Unequal cell growth, such as polar growth §Majority is purple bacteria except Planctomyces and Pirella which are unusual as their cell walls consist mainly of protein §Most are aquatic, many live attach to surfaces using prosthecae §A stalk is usually a prostheca except in Planctomyces

13. Budding and Appendaged (Prosthecate) Bacteria Contrast between cell division in conventional bacteria and in budding and stalked bacteria

14. Budding and Appendaged (Prosthecate) Bacteria A Caulobacter rosette. The five cells are attached by their stalks (prosthecae)

15. Budding and Appendaged (Prosthecate) Bacteria Stages in the Hyphomicrobium cell cycle

16. Budding and Appendaged (Prosthecate) Bacteria Physiology and Ecology §Hyphomicrobium is a methylotrophic bacteria, widespread in freshwater, marine and terrestrial habitates. Photomicrographs of cells of Hyphomicrobium

17. Spirilla Spirillum volutans Spirosoma linguale Intestinal spirillum Purple bacteria Gram-negative The genus Spirillum includes only S. volutans Spirillum volutans is a large bacteria, micro- aerobic Azospirillum lipoferum is a nitrogen-fixing organism. It can form a loose symbiotic relationship with tropical grasses and grain crops Aquaspirillum magnetotacticum has magnetotaxis ability

18. Spirilla §Aquaspirillum magnetotacticum contains particles of Fe 3 O 4 (magnetite) called magnetosomes arranged in a chain Auqasirillum magnetotacticum

19. Spirilla: Bdellovibrio Bdellovibrio bacteriovorus

20. Spirilla: Bdellovibrio §Preying on other bacteria §Attack and develop intraperiplasmically §A wide variety of gram-negative bacteria can be attacked by a single Bdellovibrio species §Gram-positive cells are not attacked §Obligate aerobe, purple bacteria (delta group)

21. Spirilla: Bdellovibrio bacteriovorus Developmental Cycle

22. Spirochetes §Typically slender, flexuous, helical in shape, often rather long §Axial fibrils or axial filaments are attached to the cell poles and wrapped around the coiled protoplasmic cylinder. §Both the axial fibrils and the protoplasmic cylinder are surrounded by a three-layered membrane called the outer sheath or outer cell envelope Spirochaeta stenostrepta Spirochaeta plicatilis Treponema pallidum causes syphilis diseases

23. Spirochetes §Arrangement of the protoplasmic cylinder, axial fibrils, and external sheath §The manner in which the rotation of the rigid axial fibril can generate rotation. Spirochaeta zuelzerae

24. Spirochetes: Classification §Six genera (based on habitat, pathogenicity, and morphological, physiological characteristics): l Spirochaeta l Cristispira l Treponema (Host in human, causes sexual disease syphilis) l Leptospira (L. Interrogans causes nephritis and jaundice) l Leptonema l Borrelia (B. recurrentis causes relapsing fever) Cristispira

25. Spirochetes §Morphology of Treponema saccharophilum Treponema saccharophilum

27. Gliding Bacteria §No flagella but can move when in contact with surfaces §Gram-negative, purple bacteria, some are Bacteroides-Flavobacterium §Myxobacteria can form multicellular structures called fruiting bodies Beggiatoa Thioploca Filamentou sulfur-oxidizing bacteria in a small stream Sulfur-oxidizing bacteria

29. Gliding Bacteria: Leucothrix §Chemoorganotrophic, requires both H2S and an organic compounds for growth §Form gonidia under unfavorable conditions §May aggregate to form rosette when gonidia have high concentration Leucothrix mucor

30. Gliding Bacteria: Fruiting Myxobacteria Myxococcus fulvus Mellitangium erectum Myxococcus stipitatus Chondromyces crocatus Stigmatella aurantiaca Exhibit the most complex behavioral patterns and life cycles of all known prokaryotes

31. Gliding Bacteria: Fruiting Myxobacterium Gliding myxobacterium Stigmatella aurantiaca Have rather large chromosome size Rely on lysis of other bacteria for nutritions Myxospores are more resistant to drying, sonic vibration, UV and heat. Usually colored by carotenoid pigments

32. Gliding Bacteria

33. §Myxobacteria have distinct growth morphology Myxococcus xanthus on agar Myxococcus fulvus slime tracks on agar Fruiting body of stigmatella aurantiaca

34. Gliding Bacteria Fruiting body formation in Chondromyces crocatus Early stage Stalk formation Three stages in head formation Mature fruiting bodies

35. Sheathed Bacteria §Filamentous organisms with a unique life cycle involving formation of flagellated swarmer cells within a long tube or sheath §Under unfavorable conditions, the swarmer cells move out and become dispersed to new environments, leaving behind the empty sheath Sphaerotilus natans Active growth stage Swarmer cells leaving the sheath Swarmer cell

36. Pseudomonads §Purple bacteria (Proteobacteria), gram-negative §Polar flagella, aerobic, grow at neutral pH mesophilically §Chemoorganotrophic, never show a fermentative metabolism §P. aeruginosa is opportunistic pathogen, some are plant pathogens Pseudomonas have broad substrate utilization, some produce polyhydroxy- butyrate and some synthesize medium- chain-length polyhydroxyalkanoates, have been studied in our lab

37. The Entner-Doudoroff pathway, the major means of glucose catabolism in pseudomonads

38. Free-Living Aerobic Nitrogen-Fixing Bacteria §Large, gram-negative, obligately aerobic rods, capable of fixing N 2 nonsymbiotically §Azotobacter has the highest respiratory rate of any living organism (purple bacteria) §Azotobacter cells are very large, almost the size of yeasts, produce cysts Vegatative cells of Azotobacter vinelandii Cysts Azotobacter cysts have low endogenous respiration and are resistant to desiccation, mechanical disintegration, and UV as well as ionizing radiation however, they are not especially heat-resistant

39. Free-Living Aerobic Nitrogen-Fixing Bacteria §Major genera: Azotobacter, Azomonas, Azospirillum and Beijerinckia, Derxia Colonies of Beijerinckia species growing on a carbohydrate-containing medium Derxia gummosa encased in slime

40. Free-Living Aerobic Nitrogen-Fixing Bacteria §Acid-tolerant, free-living N 2 -fixing Bacteria Acid-tolerant, free-living N 2 -fixing bacteria Beijerinckia indica Derxia gummosa PHB

41. Acetic Acid Bacteria §Purple bacteria, gram-negative, aerobic, motile rods, tolerance to low pH §Carry out incomplete oxidation of alcohols, leading to the accumulation of organic acids as end products §With ethanol as a substrate, acetic acid is produced Acetobacter aceti on calcium carbonate agar containing ethanol as energy source. The clearing around the colonies due to the dissolution of calcium carbonate by the acetic acid produced by the bacteria Classified as Gluconobacter Can carry out incomplete oxidation of higher alcohol and sugars: Glucose Gluconic acid Galactose Galactonic acid Arabinose Arabonic acid Sorbitol Sorbose Ascobic acid

42. Zymomonas and Chromobacterium §Facultatively aerobic gram-negative rods, beta purple bacteria §Chromobacterium violaceum produces a purple pigment violacein, a water-insoluble pigment that has antibiotic properties and is produced only from tryptophan §Zymomonas carries out fermentation of sugar to ethanol, used in beverage industry. A large colony of Chromobacterium violaceum growing among other colonies on an agar plate Pigment violacein produced by various species of the genus Chromobacterium

43. Vibrio and Related Genera Gram-negative, facultatively aerobic rods and curved rods that possess a fermentative metabolism Most are aquatic. The Group contains Vibrio, Aeromonas, Photobacterium Some can emit light (luciferase luciferase), these are associated with fish. and Plesiomonas