Slug Formation Prestalk cells Prespore cells (tagB::GFP) (cotB::GFP) leaders followers Video: 1.25 hr total time Chlorinated hexanone
Cells Assume Different Fates Depending on Their Location in The Slug Fluorescent reporters driven by differentiation- specific promoters PrestalkA::GFP and PrestalkO::RFP (i.e. subpopulations of stalk cells)
The Morphogenetic Progression (24hr)
A Few Questions for Thought Describe, in your own words, the Dictyostelium life cycle. Review your notes from the first semester on microfilament (actin)/myosin-based cell motilities and integrate that with the Dictyostelium model. Review your notes from previous quarters on cell signaling - the roles of cAMP, G-proteins, phosphoinositides (IP 3 ), Calcium ions and kinases in how cells transduce, amplify and respond to changing environmental conditions. Describe the cooperative nature of culminant formation, considering how and why certain cells contribute to the structure by forming a stalk or spores. Consider how “cheaters” emerge in this process and how that might bring an immediate reward but ultimately prove a poor strategy for life (a very Haverfordian lesson).