Presentation is loading. Please wait.

Presentation is loading. Please wait.

Biotechniques. Tissue Culture (micro-propagation) A method of rapidly cloning desired plants [few weeks]. Plant cells are totipotent (can grow into whole.

Similar presentations


Presentation on theme: "Biotechniques. Tissue Culture (micro-propagation) A method of rapidly cloning desired plants [few weeks]. Plant cells are totipotent (can grow into whole."— Presentation transcript:

1 Biotechniques

2 Tissue Culture (micro-propagation) A method of rapidly cloning desired plants [few weeks]. Plant cells are totipotent (can grow into whole plant) Process is repeated to generate millions of plants from one explant.

3 Conditions Sterile (avoid fungal and bacterial growth) essential food, mineral and vitamins auxins and cytokinins

4 Method small explant is cut and sterilised explant placed on nutrient agar medium transferred to growing medium grows into callus (mass of undifferentiated cells) hormones cause cell differentiation  new plants planted out and hardened off

5

6

7 Advantages of tissue culture copy desirable plants virus free explants prone to virus infection (can study effects of infection) cultures subjected to stress – select cells that survive (hardy) for cloning can transfer new genes into cell (with a DNA gun) prior to cloning

8 Tissue culture Do (tissue culture, 7 min) Do Culture (a bit boring, but not youtube, 7 min, sound and video way out of synch and cut off half way through?) Culture


Download ppt "Biotechniques. Tissue Culture (micro-propagation) A method of rapidly cloning desired plants [few weeks]. Plant cells are totipotent (can grow into whole."

Similar presentations


Ads by Google