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MYCOPESTICIDES Collecting and Cultivating Entomopathogens Tradd Cotter Mushroom Mountain, LLC Liberty, SC.

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Presentation on theme: "MYCOPESTICIDES Collecting and Cultivating Entomopathogens Tradd Cotter Mushroom Mountain, LLC Liberty, SC."— Presentation transcript:

1 MYCOPESTICIDES Collecting and Cultivating Entomopathogens Tradd Cotter Mushroom Mountain, LLC Liberty, SC

2 Myco- “mushroom, fungus” Pesticide- “substance that prevents, destroys, or mitigates any pest”

3 Types of Pests Insects – structural, human disease vectors, biting, agricultural Microbial – bacterial and fungal pathogens related to human and plant health Rodents

4 Types of Pests Insects – structural, human disease vectors, biting, agricultural Microbial – bacterial and fungal pathogens related to human and plant health Rodents - Monsanto, DOW, Syngenta, etc

5 Concerns with Mycopesticides Some insects are beneficial at one stage and a pest at another. Caterpillars eating agricultural crops Adult butterflies serve as beneficial pollinators

6 Concerns with Mycopesticides Host jumping Range of specificity must be determined within a clade of family of insects Mechanisms for target specificity, spore adhesion and host recognition not yet understood Residual effects

7 Benefits of Mycopesticides Narrow bandwidth of target specificity using cloned ecotypes. Self replicating – inexpensive to manufacture Low to no ecotoxicity compared to chemical pesticides Can be collected from native or local stock to target local pests

8 Copyright – Tradd Cotter

9 CHLORPYRIFOS 7.5 BILLION POUNDS OF PRODUCT USE PER YEAR ACTIVE INGREDIENT

10 Metarhizium feeding on Varroa Mites

11 Isolation and Purification of Cultures Insect Surface Sterilize insect – ethanol / sterile water wash – alternate 3 times 30 seconds each Open insect and remove tissue in appendages, not abdomen or gut Use dental tool or needle – peroxide wash tissue for 5 minutes if necessary Use antibiotic media

12 Isolation and Purification of Cultures Teleomorph – Fruiting Body Place insect and attached fungus into a humidity chamber to allow maturation of spore bearing surface Use needle to touch emerging spores from parethecia (pits) OR surface sterilize fruiting body and remove sterile tissue

13 Isolation and Purification of Cultures Maintaining Entomopathogens Capture target insects specific for fungus Blend exoskeletons into agar formulation before sterilization Host recognition and target specificity begins to deteriorate if host is not present after several transfers – lose interest Submerged agar storage on slants Liquid combined with glycerol and frozen

14 MYCOPESTICIDES WHAT WE HAVE ACCOMPLISHED Isolation of Pure Culture Death and Mummification ( 7 days ) Discovery Ant Gallery Construction Addition of Fungus ( 5 days )

15 MYCOPESTICIDES TARGET-SPECIFIC, Biological alternative to chemicals Chemical Pesticide Large Target Window MYCOPESTICIDAL Narrow Target Window Broad Range Acephate Toxicity

16 MYCOPESTICIDES PLAN OF ACTION AND CHALLENGES 2. PERMITTING AND FIELD TRIALS EPA & Clemson Pesticide Permitting Applied For 3.INDUSTRIAL DEVELOPMENT Process development and scaling from parallel models 4.LICENSE AND DISTRIBUTE Manufacture or license active ingredient 1.DNA AND PATENT PROTECTION Identify and File Delivery System or Trade Secret Process


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