Presentation on theme: "Lila N. Salhi, David Bernard-Perron, Suha Jabaji, B. Franz Lang"— Presentation transcript:
1Lila N. Salhi, David Bernard-Perron, Suha Jabaji, B. Franz Lang Microorganisms with biofertilizing and biocontrol properties in cranberriesLila N. Salhi, David Bernard-Perron, Suha Jabaji, B. Franz Lang
2Overview What are plant endophytes? Benefits of endophytes for plants Only few endophytes are knownAre there more? what are they good for?Result: endophyte diversity is completely underestimatedWhich ones are beneficial?Conclusions : Exiting future opportunities
3Why microorganisms? Every child knows (?): bacteria dissolve minerals (P, K) and make them available via plant roots.Microbial community is involved in:Stimulation of plant growthImprovement of soil fertilityHolguin et al., 1999). Ciencia y Desarrollo 25 (No.144): 26-35
4Plant growth promoting microorganisms Colonize plant roots (rhizosphere and endophytes).Dominate in competition with other microbes.Promote plant growth by:Wide spectrum of hormonal stimulation factorsBiofertilization (nutrient solubilization, nitrogen fixation)Pathogen-control (antibiotics, fungicides, insecticides …)Rothballer et al., 2003; Gamalero et al., 2004, Persello-Cartieauxet al., 2003
5Are endophytes always plant growth promoting? No!Potential interaction types(fluid, sometimes transitional):Neutral : No effectMutualistic : beneficialPathogenic : harmfulZhu et al, 2012
6Fungal endophytes of cranberries: little is known Ericoid mycorrhizaDark, septate endophytesGrowth inside plant cells.Hyphae don't extend into soilHeterogeneous groupDarkly-pigmentedSeptate hyphaeRhizoscyphus ericaeOidiodendron maiusPhialocephala fortiniiSmith and Read, 2008; Jumpponen and Trappe, 1998
7… and bacterial endophytes: even less is known Pseudomonas syringaeGram negative with polar flagellaUsually a pathogen, but …Successful in controlling Cottonball disease (Monilinia oxycocci)Pseudomonas spvoland et al, 1999
8Questions and Hypotheses A much more diverse universe of endophytes?Which isolates in our microbial collection are beneficial?How to implant them sustainably with plants ?
91. Isolation from plant tissue 3. DNA Sequencing and Blast (NCBI) Molecular identification of microorganisms1. Isolation from plant tissueITSITS1-FBMBC-FITS4-R5.8SITS1ITS228S18S600 nt750 ntIGSETSrRNA gene in fungiLeaveStemRoot2. DNA Extraction and PCRtRNA534-R27-F507 nt5S23S16SrRNA gene in bacteria3. DNA Sequencing and Blast (NCBI)BLASTMuyzer et al. (1993) ; White et al ; blast.ncbi.nlm.nih.gov
10Halo representing mineral solubilization Checking of microorganism propertiesPhosphatesolubilizationNitrogenfixationBiocontrolNBRIP mediumInsoluble phosphateNitrogenfree mediumPDA mediumHalo representing mineral solubilizationBacterial growthFungal or bacterialgrowth inhibition
17ConclusionsUnexpectedly large endophyte diversity in cranberry tissuesDifferent types of microbes in roots and aerial plant parts.Some endophytes have plant growth promoting properties.Others have strong fungicide properties.What Next?
18Thanks ! This work was possible thanks to financial support by Le ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec