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Microorganisms with biofertilizing and biocontrol properties in cranberries Lila N. Salhi, David Bernard-Perron, Suha Jabaji, B. Franz Lang.

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Presentation on theme: "Microorganisms with biofertilizing and biocontrol properties in cranberries Lila N. Salhi, David Bernard-Perron, Suha Jabaji, B. Franz Lang."— Presentation transcript:

1 Microorganisms with biofertilizing and biocontrol properties in cranberries Lila N. Salhi, David Bernard-Perron, Suha Jabaji, B. Franz Lang

2 Overview What are plant endophytes? Benefits of endophytes for plants Only few endophytes are known Are there more? what are they good for? Result: endophyte diversity is completely underestimated Which ones are beneficial? Conclusions : Exiting future opportunities

3 Every child knows (?): bacteria dissolve minerals (P, K) and make them available via plant roots. Holguin et al., 1999). Ciencia y Desarrollo 25 (No.144): Microbial community is involved in:  Stimulation of plant growth  Improvement of soil fertility Why microorganisms?

4 Plant growth promoting microorganisms  Colonize plant roots (rhizosphere and endophytes).  Dominate in competition with other microbes.  Promote plant growth by: Rothballer et al., 2003; Gamalero et al., 2004, Persello-Cartieauxet al., Wide spectrum of hormonal stimulation factors 2.Biofertilization (nutrient solubilization, nitrogen fixation) 3.Pathogen-control (antibiotics, fungicides, insecticides …)

5 Are endophytes always plant growth promoting? No! Potential interaction types (fluid, sometimes transitional):  Neutral : No effect  Mutualistic : beneficial  Pathogenic : harmful Zhu et al, 2012

6 Fungal endophytes of cranberries: little is known Ericoid mycorrhiza  Growth inside plant cells.  Hyphae don't extend into soil Rhizoscyphus ericae Oidiodendron maius Dark, septate endophytes Smith and Read, 2008; Jumpponen and Trappe, 1998 Phialocephala fortinii  Heterogeneous group  Darkly-pigmented  Septate hyphae

7 … and bacterial endophytes: even less is known Pseudomonas syringae  Gram negative with polar flagella  Usually a pathogen, but …  Successful in controlling Cottonball disease (Monilinia oxycocci) voland et al, 1999 Pseudomonas sp

8 Questions and Hypotheses A much more diverse universe of endophytes? Which isolates in our microbial collection are beneficial? How to implant them sustainably with plants ?

9 tRNA 534-R 27-F 507 nt 5S tRNA 23S16S rRNA gene in bacteria BLAS T 2. DNA Extraction and PCR Muyzer et al. (1993) ; White et al. 1990; blast.ncbi.nlm.nih.gov ITS ITS1-FBMBC-F ITS4-R 5.8S ITS1 ITS2 28S 18S 600 nt 750 nt 28SIGS5.8S 18S ETS rRNA gene in fungi Molecular identification of microorganisms Root Stem Leave 3. DNA Sequencing and Blast (NCBI) 1. Isolation from plant tissue

10 Checking of microorganism properties Halo representing mineral solubilization Phosphate solubilization Nitrogen fixation NBRIP medium Insoluble phosphate Nitrogen free medium Bacterial growth Biocontrol PDA medium Fungal or bacterial growth inhibition

11 Results 1 – Microbial Diversity 2 – Beneficial Proprieties

12 Unexpected endophyte diversity: the tip of an iceberg? 137 microorganisms isolated Roots 65 Fungi, 36 Bacteria Fungi Trimmatostroma betulinum Not identified Bacteria : Bacillus sp. Paenibacillus sp Pantoea sp Roots Stems Leaves Others

13 Stems 16 Fungi, 8 Bacteria Alternaria sp. Nemania serpens Fungi: Paenibacillus sp. Bacteria : Roots Stems Leaves Others

14 Leaves 8 Bacteria Bacillus subtilis Bacteria : Roots Stems Leaves Others

15 Phosphate solubilization Nitrogen fixation Bacillus subtilis Burkholderia sp. Rahnella aquatilis Rhodococcus qingshengii Burkholderia sp. Rahnella aquatilis Panteoa sp. Cohnella ginsengisoli Rhizobium sp. Which endophytes may be beneficial?

16 Biocontrol Pseudomonas sp. Penicillium janthinellum Bacterial-fungal interaction Fungal-fungal interaction

17 Conclusions  Unexpectedly large endophyte diversity in cranberry tissues  Different types of microbes in roots and aerial plant parts.  Some endophytes have plant growth promoting properties.  Others have strong fungicide properties. What Next?

18 Thanks ! This work was possible thanks to financial support by Le ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec


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