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Secrets of the Rainforest. The Mysterious Green Florescent Leaves  While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal.

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Presentation on theme: "Secrets of the Rainforest. The Mysterious Green Florescent Leaves  While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal."— Presentation transcript:

1 Secrets of the Rainforest

2 The Mysterious Green Florescent Leaves  While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal green leaves from Ramon  You bring back to Biotex, a biotech company working on a cure for cancer Hi, I am Tisha

3 Green Florescent Protein  Biotex scientists determine that the leaves contain a florescent green protein  DNA from the leaves is inserted into bacterial cells

4 Cloning the Gene from Mysterious Leaves

5 More on Cloning the Gene…

6 Purifying the GFP  Biotex technicians need to separate the GFP from other bacterial proteins  Column Chromatography separates contaminating proteins from GFP  The result is a purified GFP that can be tested for its ability to cure stomach cancer

7 Testing and Placebos  GFP will need to be tested on rats before it is allowed for a human trial  The US Food and Drug Administration will examine Biotex’s experiments to determine if the protein is safe and cures stomach cancer without negative side effects

8 Ethics and Economics  Once GFP is approved by the FDA, you will develop GFP as a commercial product  You will need to be aware of the following viewpoints: FDA, social conscience, patient advocacy groups, and the employees of Biotex  You must resolve the dilemmas in order to successfully market GFP

9 Final Presentations  Turn in all lab work with analysis questions  Video Presentation of your marketing of GFP  Address issues of all dilemmas 1-8

10 Background Information- Lesson 1  How to use Pipettes  Streaking of agar plates to Produce Single Colonies (Sterile Technique)

11 Background Continued…Lessons 2 & 3  Process of Transformation (GMOs or genetic recombinants)  Jellyfish, GFP and pGLO and Fluorescence  Isolating Clones to Propagate in Liquid Culture Media

12 Background Continued…Lesson 4  Concentration of Bacteria from Liquid Culture Media

13 Background Continued…Lesson 5  Prepare a Bacterial Lysate for Protein Chromatography

14 Background Continued…Lesson 6  Protein Chromatography- Hydrophobic Interaction Chromatography (HIC)  GFP is hydrophobic and will stick to hydrophobic beads in salt water buffer  When salt is removed, GFP will then be collected  4 buffers:  1) Equilibration buffer (salt buffer)  2) Binding Buffer (high salt)  Wash buffer (medium salt)  Elution buffer (low salt)

15 Background Continued…Lessons  Lesson 7- testing and placebos  Lesson 8- Advocacy Groups and Viewpoints  Lesson 9-Ethics and Economics  Lesson 10- Preparing and Giving Presentations


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