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SMASH 2008 Recap: Introducing Citation, Your Hard- Working Lab Assistant Dean Olson, Ph.D. Director of Customer Support Protasis Corporation.

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Presentation on theme: "SMASH 2008 Recap: Introducing Citation, Your Hard- Working Lab Assistant Dean Olson, Ph.D. Director of Customer Support Protasis Corporation."— Presentation transcript:

1 SMASH 2008 Recap: Introducing Citation, Your Hard- Working Lab Assistant Dean Olson, Ph.D. Director of Customer Support Protasis Corporation

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4 Citation: Push-Button Sample Loader Chromatography-grade Fluidics Easy-to-use, Web-based Software Built-in Instrument Controller Integral Degassing Field-serviceable Valves Small-footprint benchtop packaging Enjoy the reassurance of feedback control, auto-calibration, and system self-maintenance in applications where the robot is not required Completely upgradeable to full robotic automation

5 Chromatography grade pumps Field-serviceable valves Integrated feedback control sensors Degas, blanket, sparge On-board instrument controller Protasis One-Minute™ NMR Lite Software

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7 The size of a plant metabolome is unknown it is estimated at a few thousand constituents Plant metabolomics LC-NMR mol LC-UV10 -9 mol GC-MS mol LC-MS mol LC-LIF mol CE-LIF mol Sumner et al. Phytochemistry 2003, 62, Rapid, Reproducible and Non-Destructive Low Sensitivity NMR Metabolomics High Sensitivity Instrumentation variability LC-MS: problem in RT repeatability MS Metabolomics

8 CONTROL WOUNDED PCA of total crude extract MS spectra spiked Total crude extract TOF MS spectra RANKED LIST OF INDUCED IONS Total crude extract TOF MS spectra CONTROL WOUNDED C 12 H 17 O Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

9 UPLC-TOF-ES-MS of A. thaliana Induced by wounding m/z (+/- 10 ppm) m/z (+/- 0.5 Da) CAP-NMR Multiple collection triggered by MS Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

10 CAP-NMR of ion m/z 209 CAP-NMR ca 1  g NT= ,2,811,5412 Standard 50  g Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

11 Further example details can be found in… A LC-MS approach is presented for the isolation of minor key plant biomarkers, in view of their characterization by NMR at the microgram scale …using ultra-performance liquid chromatography- time-of-flight mass spectrometry (UPLC-TOF-MS) … then transferred to semi-preparative LC conditions with MS detection … and subsequent characterization by capillary NMR (CapNMR).

12 Hot Off the Press: Natural Products Workflow Herein we present a method of extract evaluation using HPLC profiling with biological evaluation 16 followed by capillary probe NMR spectroscopy/ESMS/UV combined with NMR database (AntiMarin) evaluation, which reduces the crude extract requirement for dereplication to submilligram quantities. 17

13 Full Robotic Automation Automated Push-Button Discrete Sample Loading vs. You pick


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