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1Housekeeping Tidy up lab space –Discard razor blades & used slides in labeled waste containers –Discard/clean-up Analine blue slides –Ensure fans, cords,

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Presentation on theme: "1Housekeeping Tidy up lab space –Discard razor blades & used slides in labeled waste containers –Discard/clean-up Analine blue slides –Ensure fans, cords,"— Presentation transcript:

1 1Housekeeping Tidy up lab space –Discard razor blades & used slides in labeled waste containers –Discard/clean-up Analine blue slides –Ensure fans, cords, etc. are neat and away from walkways –No food. –Ensure analine blue and acid containers are closed

2 2Announcements First plate is due today –Transfer to lab computer –Get instructor feedback Discard foul water/nasty seaweed –Don’t leave seaweeds in closed containers (i.e., baggies) Use fans to speed-up herbarium drying –Overnight –While we’re in the field

3 3Logistics Tuesday, 15 July –AM: Introduction to Green Algae (Wysor) –PM: Gracilariaceae & Gracilaria spp. (Fredericq) Wednesday, 16 July –AM: Orders of Green Algae (Wysor) –PM: Gracilariaceae & Gracilaria spp., cont’d (Fredericq) Thursday, 17 July –AM: Turf Algae & Endophytes (Wysor) –PM: Crude isolations for Algal Culture Friday, 18 July –Off day…SCUBA? Saturday, 19 July –AM: Trees, Phylogenies & other ventures on the dark side (Freshwater) Sunday, 20 July –AM: Poly- & Neosiphonia: Molecular assisted morphological analysis –PM: Finalize Plates Monday, 21 July –AM: plate presentations –All plates due by 12:00 –PM: Tuesday, 22 July –AM: 8am departure for Zapatilla snorkelling (no lecture) (cameras, no baggies) –PM:Wrap-up/Clean-up lab Wednesday, 23 July (Last day of class) –AM: Wrap-up/start to panic/pack/clean- up lab –PM: finish panicking/update on barcoding progress Thursday, 24 July: Departure

4 4 Chlorophytes - “grass” green algae

5 5 Nuisance Algae - “Green Tides” Codium fragile –aka: Deadman’s fingers –aka: Oyster theif

6 6 Nuisance Algae - “Green Tides” Ulva bloom in Greenwich Bay, RI

7 7 Nuisance Algae - Hull Fouling

8 8 Trentepohlia sp. - an orange Chlorophyte Carotenoid pigments, such as Astaxanthin &  -carotene impart orange color

9 9 Haematococcus sp. - an orange Chlorophyte

10 10 Commercial Astaxanthin Production Haematococcus pluvialis

11 11 Commercial Astaxanthin Production

12 12 Chlamydomonas nivalis - a red snow Chlorophyte For more information on snow algae: –http://www.orgs.muohio.edu/uvlakes/UVecology/Phyto/snowalg.html –http://www.chikyu.ac.jp/takeuchi/snowalgae_ak.html Alaska, USASierra Nevadas, USA

13 13 Ulothrix: an unbranched, uniseriate, green algal filament (Ulvophyceae: Ulotrichales) General Characteristics of Green Algae Variation in algal thallus (algal body) –filaments (filamentous)

14 14 Bryopsis: a siphonous, multi-nucleate, unicellular green alga (Ulvophyceae: Caulerpales) No cross-walls. General Characteristics of Green Algae Variation in algal thallus (algal body) –siphonous or coenocytic: one large multi- (or uni-) nucleate cell

15 15 Acetabularia: a siphonous, uni-nucleate, unicellular green alga (Ulvophyceae: Dasycladales) Codium fragile: a siphonous, multi-nucleate, unicellular green alga (Ulvophyceae: Caulerpales) General Characteristics of Green Algae Variation in algal thallus (algal body) –siphonous or coenocytic: one large multi- (or uni-) nucleate cell

16 16 General Characteristics of Green Algae Ulva: a parenchymatous, distromatic blade (Ulvophyceae: Ulvales) Variation in algal thallus (algal body) –parenchymatous blade

17 17 Anadyomene: a pseudoparenchymatous, blade (Ulvophyceae: Cladophorales) General Characteristics of Green Algae Variation in algal thallus (algal body) –pseudoparenchymatous blade

18 18 Parallel Evolution of Thallus Form - Uniseriate Filaments Ulothrix Class: Chlorophyceae Ulvophyceae See also G&W: Fig. 17-8

19 19 Parallel Evolution of Thallus Form - Monostromatic Blades Class: Ulvophyceae Ulvophyceae Order: Ulvales Ulotrichales Genus: Ulvaria Monostroma See also G&W: Fig. 17-8

20 20 Phylogeny & Synopsis of Green Algae Prasinophyceae –marine flagellates –primitive (ancestral type) green algae Chlorophyceae –FW, terrestrial –lots of unicells/colonies, e.g., Volvox Trebouxiophyceae –Terrestrial, fw –lichen symbionts (among others) Charophyceae –FW –land plant (drier green algae) sisters Ulvophyceae –Marine, macroscopic seaweeds (among others)

21 21 Given the problems associated with convergent evolution (parallel evolution of thallus form)… –what types of (really conserved) features might be used to differentiate major groups of organisms? What are some features common to all organisms/groups of organisms?

22 22 Flagella Ultrastructure of Green Algae See also: G&W. Fig. 9.8c

23 23 Ultrastructure of the Flagellar Apparatus

24 24 Understanding Basal Body Orientation 1.Orientation is assumed to be top-down

25 25 Understanding Basal Body Orientation 2-microtubule rootlet Basal body: cylindrical basal portion of flagellum lying within the cell 4-microtubule rootlet Flagellate cells in the UTC clade have a symmetrical, cruciate root systems with variable #s of alternating rootlets.

26 26 2. Microtubule rootlets anchor basal bodies to cytoskeleton 3. A cruciate (cross-shaped) arrangement of rootlets is shown (X-2-X-2); ( ) Understanding Basal Body Orientation

27 27 4. There are 3 main variations in the orientation of basal bodies, when viewed top-down (anterior- posterior direction) 5. Orientation is a round a line drawn parallel to and between basal body axis Understanding Basal Body Orientation

28 28 Clockwise Displacement of Basal Bodies CW Chlorophyceae Clockwise 1:00 - 7:00

29 29 Counter Clockwise Displacement of Basal Bodies CCW Counter-clockwise 11:00 - 5:00 Ulvophyceae

30 30 Understanding Basal Body Orientation Chlorophyceae Ulvophyceae Trebouxiophyceae Clockwise 1:00 - 7:00 Counter-clockwise 11:00 - 5:00 Directly opposed

31 31 Flagellar Apparatus in Classes of Green Algae

32 32


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