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Efficacy of Various Insecticides Against Bed Bugs Dr. Bill Donahue Sumiko De La Vega Nebraska Urban Pest Management Conference February 12-13, 2013.

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Presentation on theme: "Efficacy of Various Insecticides Against Bed Bugs Dr. Bill Donahue Sumiko De La Vega Nebraska Urban Pest Management Conference February 12-13, 2013."— Presentation transcript:

1 Efficacy of Various Insecticides Against Bed Bugs Dr. Bill Donahue Sumiko De La Vega Nebraska Urban Pest Management Conference February 12-13, 2013

2 “Brood Centers” Bed bugs are thigmotactic preferring rough surfaces such as wood or paper. Cluster together in contact with each other where fecal matter, egg shells and exuvia accumulate - Semiochemicals Return to these harborages after a blood meal. Quiescent state while digestion takes place. Stimulus to leave harborage? Hunger?

3 “Brood Centers”

4 Repellent A chemical that makes unattractive to arthropods a habitat, food source, or oviposition site ordinarily sought and frequented. “Push-Pull” Theory – Repel and Attract - used in the same environment

5 Objective To evaluate the contact activity (mortality, repellency, egg distribution and hatch) of selected strains of bed bugs exposed to treated fabric substrates.

6 Chemicals Evaluated Permethrin Impregnated Fabrics (3) – 0.52% a.i. Phantom Aerosol – Chlorfenapyr 0.5% Alpine Aerosol – Dinotefuran 0.5% Bedlam Aerosol – Sumithrin 0.4%, MGK % Bed Bug Gel - Lactic Acid 20%, Rosemary Oil 1%, Hops Flower Essential Oil 1%

7 Bed Bug Strains “Harlan” – Laboratory Susceptible Strain (Ft. Dix, NJ SRL 2008) Adults Mixed Sex Ratio “Earl” – SRL Field Collected (Modesto, CA ) Susceptible Strain – Adults Mixed Sex Ratio “James” – SRL Field Collected (Modesto, CA ) Strain, 7X Permethrin Resistant – Adults Mixed Sex Ratio

8 Test System * Forced Exposure non-choice test Aerosol “Choice Test” Permethrin Fabric “Choice Test”

9 Average percent mortality of adult bed bugs (2 Strains) confined to three types of permethrin treated fabrics (“Non- Choice”) compared with an untreated control as a continuous forced exposure lethal time (LT) evaluation Fabric Type 1 hr Harlan 1 hr James 2 hr Harlan 2 hr James 4 hr Harlan 4 hr James 24 hr Harlan 24 hr James #1 Beige UPH #2 Black Stitch #3 Knit Ticking #4 UTC Woven

10 Average percent mortality of adult bed bugs (Harlan) confined to three types of permethrin treated fabrics (“Choice Test”) compared with an untreated control as a continuous exposure lethal time (LT) evaluation Fabric Type4 hr24 hr48 hr72 hr96 hr #1 Beige UPH #2 Black Stitch #3 Knit Ticking #4 UTC Woven 00000

11 Total # of Eggs, average percent hatch and average percent nymph mortality of bed bugs (Harlan) confined to three types of permethrin treated fabrics (“Choice Test”) compared with an untreated control as a continuous exposure evaluation Fabric Type Treated Side Total # Eggs Untreated Side Total # EggsAvg.% Hatch Avg. % Nymph Mortality #1 Beige UPH #2 Black Stitch #3 Knit Ticking #4 UTC Woven

12 Mortality, distribution, average percent hatch and average percent nymph mortality of bed bugs (Harlan) confined to treated mattress ticking (“Choice Test”) compared with an untreated control as a continuous exposure evaluation Treatment Max. Adult % Mort 96 hr % Adults Untreated SideAvg % Distribution EggsAvg % Hatch Avg % Nymph Mortality 4 hr96 hrTreatedUntreated Chlorfenapyr Aerosol Dinotefuran Aerosol Sumithrin Aerosol Lactic Acid + Essential Oils

13 Conclusions Reliable and predictive small scale bioassay Sensitive – bed bug strains, chemicals (actives & formulations) Demonstrates repellency – adults/nymphs, oviposition sites – “Push-Pull” applications, but need to understand the dynamics when including the host and harborage in human habitats Evaluate control options based on unique bed bug behavior – “Brood Centers” - Urban IPM

14 Questions?


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